LOCUS       sequence-372225-       11856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS expression of dSarm(isoE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 11856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-dsarm(E)
FEATURES             Location/Qualifiers
     source          1..11856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(66..170)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(196..651)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(333..354)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     545..564
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     778..800
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     792..809
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     793..809
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     816..835
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        816..834
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(868..886)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1338..1409,1811..2084,2159..2813,2875..3190,3394..3525,
                     3596..4210)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4985..5018
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5068..5162
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5181..5419
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5366..5387
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             5833..5853
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     intron          8885..8950
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9080..9100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9372..9506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9409..9428)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9463..9482
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9573..9642
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9861..9879)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9900..9916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9900..9916)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9913..9935)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9924..9940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9948..9954,9955..9972,9973..9978))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9993..10014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10131..10148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10302..10890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10382..10401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11061..11852,11853..11856,1..65))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11684..11703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
       61 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
      121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
      181 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
      241 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      301 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
      361 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
      421 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
      481 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
      541 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
      601 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
      661 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      721 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      781 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
      841 tgggtacgta ccgggcccct agtatgtatg taagttaata aaacccattt ttgcggaaag
      901 tagataaaaa aaacattttt tttttttact gcactggata tcattgaact tatctgatca
      961 gttttaaatt tacttcgatc caagggtatt tgatgtacca ggttctttcg attacctctc
     1021 actcaaaatg acattccact caaagtcagc gctgtttgcc tccttctctg tccacagaaa
     1081 tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc gccaccgttt gtagcgttac
     1141 gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg aagctccaag
     1201 cggtttacgc catcaattaa acacaaagtg ctgtgccaaa actcctctcg cttcttattt
     1261 ttgtttgttt tttgagtgat tggggtggtg attggttttg ggtgggtaag caggggaaag
     1321 tgtgaaaaat cccggcaatg ggccaagagg atcaggagct attaattcgc ggaggcagca
     1381 aacacccatc tgccgagcat ctgaacaatg tgagtagtac atgtgcatac atcttaagtt
     1441 cacttgatct ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga
     1501 cccacaaaaa tcccaaaccg caattgcaca aacaaatagt gacacgaaac agattattct
     1561 ggtagctgtt ctcgctatat aagacaattt ttgagatcat atcatgatca agacatctaa
     1621 aggcattcat tttcgactat attctttttt acaaaaaata taacaaccag atattttaag
     1681 ctgatcctag atgcacaaaa aataaataaa agtataaacc tacttcgtag gatacttcgg
     1741 ggtacttttt gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct
     1801 atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca
     1861 ggccaaaaac tacggcacgc tccggccacc cagtccgccg gaggactccg gttcagggag
     1921 cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt
     1981 caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa
     2041 cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc
     2101 agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt
     2161 tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag
     2221 acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc
     2281 gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc
     2341 tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc
     2401 caagccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat
     2461 caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc
     2521 agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggctcta
     2581 accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc ctttaccgcc
     2641 cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc
     2701 attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag
     2761 ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt
     2821 cgatgtgttt attaagggta tctagtatta cataacatct caactcctat ccagcgtggg
     2881 tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt
     2941 gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgccat
     3001 tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatctgg agaagccact
     3061 ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt
     3121 cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat
     3181 tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa
     3241 acaaattcgc tagattttag atagaattgc ctgattccac acccttctta gtttttttca
     3301 atgagatgta tagtttatag ttttgcagaa gataaataaa tttcatttaa ctcgcgaata
     3361 ttaatgagat gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc
     3421 tttttgggcc aacaactcac gcaagtgggt gtgatgaata tcaacggagc catcttcctc
     3481 ttcctgacca acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcatgtttag
     3541 aatacatttg catttcaata atttactaac tttctaatga atcgattcga tttaggtgtt
     3601 cacctcagag ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac
     3661 atactttctg ggcaaaacga ttgccgaatt gccgcttttt ctcacagtgc cactggtctt
     3721 cacggcgatt gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg
     3781 cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg
     3841 cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct
     3901 gctctttggc ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc
     3961 gtacctctca tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt
     4021 ggagccgggc gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt
     4081 catcctggag acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc
     4141 cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg
     4201 caaggagtag ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac
     4261 catcgtgttt actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata
     4321 aaaacaagat atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga
     4381 ctttggattt gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc
     4441 gtttatcgct acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc
     4501 gccctcttca taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc
     4561 cacccgctct ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag
     4621 ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat
     4681 tagtaataaa tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct
     4741 cacagctata tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg
     4801 ttcggagtga ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg
     4861 tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac
     4921 aactataata ataagaatac atttaattta gaaaatgctt ggatttcact ggaactaggc
     4981 tagcataact tcgtataatg tatgctatac gaagttatgc tagcggatcc aaaagctttc
     5041 cggatccaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     5101 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     5161 agcggagact ctagcgagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     5221 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     5281 gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag
     5341 taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga
     5401 atacaagaag agaactctga atagggaatt gggaattcat gtccaatcag gcaccctggc
     5461 ccgttcgcaa gggcatcttc cgctcgagcg gtcagtcgga tttcacgcca acccgatcgc
     5521 ctagtcccat tgtggagatg ccattatccc cgccgccggt ggccacgccc tcgaatagat
     5581 ttggaatcaa ttcaccgctc tccccaccgc cgcagcctat tcaagtggtg gcgggttcaa
     5641 cgacagccac taccatgtcc actgcgtccg ctgccagagt gtctggtgca gctgcctcca
     5701 cttcatcctc gtcctcctct tcttcgtcgt cgcagtgctc gtcgcaaacc tcgtcgagtt
     5761 ctagttcgca caccagagta cgtaaaagtt ctaacccgcc cccacagcca attaccagtt
     5821 gtccactgtc gccaccacca ccaccaccac cacagcaaca acaacaacta ccacaacaac
     5881 tgccacctcc aacgcccatc aacaacacca accactcagc aattacaaca cccaatcaca
     5941 acaataacca caactgcaat caaatgagaa atgtacgcga aattcccatt gaggtggagc
     6001 agagcaagga agccctctca ctccgcaccg gcgacatcac acagcaggcg agcaacaatg
     6061 tggcggcctc cagtattacg gtgcaaagtg agaatttctc cgcggacaag aaggcgatat
     6121 cccagtcgca gcaatcgcag acgatgacct ccaacgggat catcagtcag gagaaacatg
     6181 tatcctccgc ctcgcaggcc aactactcga tgtcgcacaa gggcgtttcc agtaccggca
     6241 gtagcatgat caccagctcc tcgcaaatgt ccgcgatgaa tggccagatg ctgaagctcg
     6301 ccgatctcaa gttggatgac ctcaagtcgc tgacagctgg cagtgggcag caggagatcg
     6361 agcagaccat caacaagtat tccaatatgc tgacctcaat agtgagctcc ctgcaagaag
     6421 atgagcgagg tggtagtgcc ataaccgtcc acgatgttgg tggcaagaag tctcagtacc
     6481 tggagaagat caacgaagtc attcgtcgag cttgggcagt gcccacacat ggccacgagc
     6541 tgggctactc attgtgcaat tccctgcgcc aaagtggcgg cctcgatctt ctcatgaaga
     6601 actgcgttaa gcccgatctc cagttttcct ccgcacagct tcttgagcag tgcctaacca
     6661 ccgagaaccg caaacatgtg gtggataatg gtctggataa ggtggtcaat gtggcctgtg
     6721 tctgcacaaa gaattccaat atggagcact cccgcgtggg cactggcatc ctggagcatc
     6781 tgttcaaaca ctccgagggc acctgttcgg atgtgataag gctaggtggt ctggatgccg
     6841 tgctctttga gtgccgcacc agtgatttgg aaactctgcg acactgcgct agtgcactgg
     6901 caaatttatc tctatatggt ggagccgaga atcaggagga gatgatccta cgcaaggtgc
     6961 ccatgtggtt gttcccattg gccttccaca acgatgataa catcaagtac tatgcctgct
     7021 tggccattgc agtgctggta gccaacaagg agattgaagc cgaggtgctg aagtctggat
     7081 gcttggattt ggtggaaccc tttgtcacct cccacgaccc ctcggctttt gcgagatcca
     7141 atctggcaca tgcccacggg caaagcaaac attggctgaa aagattggtt ccagttttga
     7201 gttccaatcg cgaagaggcc cgcaatctgg ctgccttcca tttttgcatg gaggcgggca
     7261 tcaagaggga gcagggtaac accgacatct tccgcgagat caacgctatt gaagctttga
     7321 aaaatgtggc cagctgtcca aatgccattg catccaagtt cgccgctcag gctctgagat
     7381 tgattggaga gacagttccg cacaagctgt cccaacaggt tcccttgtgg tccgtggagg
     7441 atgtgcagga gtgggtaaag caaatcggtt tcaatgacta catcgacaag ttcaacgagt
     7501 cccaggtgga cggggatctt ttactgaagc tcaatcagga taacctccgg gctgatattg
     7561 gaatcggtaa tggaatactt ctaaagcgtt ttgaacgcga gctgcaaaat ctcaagcgta
     7621 tggctgacta ctcatccaag gacacggcca agatgcacca atttctctcg gagatcggta
     7681 ctgattactg cacctacacg tatgccatgc tgaatgctgg aattgacaag tgtgcactgc
     7741 cgcatgtcaa tgaggatatg ctgatgacag agtgtggcat ccacaactcg atccatcgtc
     7801 tgagaattct caatgcggtc aaaaacttgg agaattcgct gcccagctcg tcggaggaga
     7861 acatggccaa gacgctagat gttttcgtta gctataggcg atcaaacggc tcccaactag
     7921 ccagtcttct caaggtgcat ctgcagcttc gtggtttctc cgttttcatc gacgtggagc
     7981 gcctggaggc gggcaaattt gacaacggcc tattgaacag tattcgacag gcaaagaact
     8041 ttgtcttagt attaactccc gatgccctgc accgctgcat taacgacgag gactgcaagg
     8101 actgggtgca tcgcgaaatc gtggctgccc tgaactcgaa ctgcaacatt atacccatca
     8161 tagaccagca attcgattgg cccgaggtgg agcgacttcc cgaggacatg cgcagtgtgg
     8221 cccattttaa cggtgttaat tggatccacg actaccagga cgcatgcatc gataagctcg
     8281 aaagattttt gcgcggcgaa aagaatatcg atcgcattgc ggcgatggtg cccggcaccc
     8341 ccggttcggt ctcataccaa agaatgcaca gcaacgactc cgactaccag agcggaggag
     8401 caggcgccgg atcaggagca ggaactggag gaggaggtgg tggcggcgtt acgggcagtg
     8461 tggtggatgg cctgatggtg gcggccaatg gcagcggaca aggtgggcca acgagtacta
     8521 catctactac ttcttctact cccaactcaa attcaagctc aagctctaac cagagtcccc
     8581 cagcggctcc agcgtaggaa ttccggcgat gctgatccga gcagtccgat ggggaggagg
     8641 aggaggagga ggaggacggc gatgaggagg aggaagatga tggatccgat gaggaaaatg
     8701 gaaatggaaa tagagggggc agaaatcgac ccagagacaa cggaggatga gatgatgatc
     8761 ccacatagac ttgttgcatt aaacgaatat tagcgcttat gtctagagga tctttgtgaa
     8821 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     8881 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     8941 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     9001 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     9061 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     9121 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     9181 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     9241 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     9301 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     9361 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9481 tcaatgtatc ttatcatgtc tggatcggat ccgcggccgc ggctcgaggg tacctctaga
     9541 gatccactag tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc
     9601 gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac
     9661 gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc
     9721 tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt
     9781 gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacacta
     9841 gttctagcca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
     9901 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9961 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    10021 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    10081 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    10141 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10201 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10261 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10321 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10741 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10801 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10861 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10921 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10981 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    11041 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    11101 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    11161 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11221 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11281 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11341 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11401 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11461 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11521 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11581 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11641 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11701 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11761 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11821 gcatctttta ctttcaccag cgtttctggg tgagca
//
