LOCUS       sequence-372226-       11880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS expression of dSarm(isoE)::FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-dsarm(E)::flag
FEATURES             Location/Qualifiers
     source          1..11880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(30..134)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(160..615)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(297..318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     509..528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     742..764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     756..773
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     757..773
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     780..799
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        780..798
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(832..850)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1302..1373,1775..2048,2123..2777,2839..3154,3358..3489,
                     3560..4174)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4949..4982
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5032..5126
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5145..5383
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5330..5351
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             5797..5817
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             8559..8582
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          8873..8938
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9068..9088
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9360..9494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9397..9416)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9451..9470
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9561..9630
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9849..9867)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9888..9904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9888..9904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9901..9923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9912..9928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9936..9942,9943..9960,9961..9966))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9981..10002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10119..10136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10290..10878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10370..10389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11049..11840,11841..11880,1..29))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11672..11691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
       61 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
      121 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
      181 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
      241 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
      301 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
      361 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
      421 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
      481 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
      541 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
      601 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
      661 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
      721 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
      781 aatacgactc actatagggc gaattgggta cgtaccgggc ccctagtatg tatgtaagtt
      841 aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg
      901 gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt
      961 accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt
     1021 tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc
     1081 tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cattttgtca
     1141 gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc
     1201 caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt
     1261 tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg
     1321 agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta
     1381 gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg caacatcaaa
     1441 ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaattg cacaaacaaa
     1501 tagtgacacg aaacagatta ttctggtagc tgttctcgct atataagaca atttttgaga
     1561 tcatatcatg atcaagacat ctaaaggcat tcattttcga ctatattctt ttttacaaaa
     1621 aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata
     1681 aacctacttc gtaggatact tcggggtact ttttgttcgg ggttagatga gcataacgct
     1741 tgtagttgat atttgagatc ccctatcatt gcagggtgac agcggagcgg cttcgcagag
     1801 ctgcattaac cagggcttcg ggcaggccaa aaactacggc acgctccggc cacccagtcc
     1861 gccggaggac tccggttcag ggagcggcca actagccgag aacctcacct atgcctggca
     1921 caatatggac atctttgggg cggtcaatca gccgggctcc ggatggcggc agctggtcaa
     1981 ccggacacgc ggactattct gcaacgagcg acacataccg gcgcccagga aacatttgct
     2041 caagaacggt gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc
     2101 caattaccaa tttgaaactc agtttgcggc gtggcctatc cgggcgaact tttggccgtg
     2161 atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg
     2221 cagggcatcc aagtatcgcc atccgggatg cgactgctca atggccaacc tgtggacgcc
     2281 aaggagatgc aggccaggtg cgcctatgtc cagcaggatg acctctttat cggctcccta
     2341 acggccaggg aacacctgat tttccaagcc atggtgcgga tgccacgaca tctgacctat
     2401 cggcagcgag tggcccgcgt ggatcaggtg atccaggagc tttcgctcag caaatgtcag
     2461 cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt
     2521 ctggcattcg cctccgaggc tctaaccgat ccgccgcttc tgatctgcga tgagcccacc
     2581 tccggactgg actcctttac cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag
     2641 aagggcaaga ccgtcatcct gaccattcat cagccgtctt ccgagctgtt tgagctcttt
     2701 gacaagatcc ttctgatggc cgagggcagg gtagctttct tgggcactcc cagcgaagcc
     2761 gtcgacttct tttcctagtg agttcgatgt gtttattaag ggtatctagt attacataac
     2821 atctcaactc ctatccagcg tgggtgccca gtgtcctacc aactacaatc cggcggactt
     2881 ttacgtacag gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc
     2941 caagatatgc gacaattttg ccattagcaa agtagcccgg gatatggagc agttgttggc
     3001 caccaaaaat ctggagaagc cactggagca gccggagaat gggtacacct acaaggccac
     3061 ctggttcatg cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc
     3121 actcctcgta aaagtgcgac ttattcagac aacggtgagt ggttccagtg gaaacaaatg
     3181 atataacgct tacaattctt ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt
     3241 ccacaccctt cttagttttt ttcaatgaga tgtatagttt atagttttgc agaagataaa
     3301 taaatttcat ttaactcgcg aatattaatg agatgcgagt aacattttaa tttgcagatg
     3361 gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt gggtgtgatg
     3421 aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc
     3481 acgataaatg taagtcatgt ttagaataca tttgcatttc aataatttac taactttcta
     3541 atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg
     3601 aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg aattgccgct
     3661 ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc
     3721 cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac
     3781 gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg
     3841 tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt
     3901 gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct
     3961 gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac
     4021 cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct
     4081 gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct
     4141 ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt
     4201 gttttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct
     4261 aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc
     4321 ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac
     4381 ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt
     4441 atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt
     4501 cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga
     4561 cgaccttgga gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt
     4621 ggttcatttc gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc
     4681 caatatattt gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt
     4741 tttaaggtaa ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt
     4801 gacatccagt gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg
     4861 ttgtttatat atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat
     4921 gcttggattt cactggaact aggctagcat aacttcgtat aatgtatgct atacgaagtt
     4981 atgctagcgg atccaaaagc tttccggatc caagcttgca tgcctgcagg tcggagtact
     5041 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
     5101 ctccgagcgg agtactgtcc tccgagcgga gactctagcg agcgccggag tataaataga
     5161 ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag
     5221 cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc
     5281 aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga
     5341 aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg aattgggaat
     5401 tcatgtccaa tcaggcaccc tggcccgttc gcaagggcat cttccgctcg agcggtcagt
     5461 cggatttcac gccaacccga tcgcctagtc ccattgtgga gatgccatta tccccgccgc
     5521 cggtggccac gccctcgaat agatttggaa tcaattcacc gctctcccca ccgccgcagc
     5581 ctattcaagt ggtggcgggt tcaacgacag ccactaccat gtccactgcg tccgctgcca
     5641 gagtgtctgg tgcagctgcc tccacttcat cctcgtcctc ctcttcttcg tcgtcgcagt
     5701 gctcgtcgca aacctcgtcg agttctagtt cgcacaccag agtacgtaaa agttctaacc
     5761 cgcccccaca gccaattacc agttgtccac tgtcgccacc accaccacca ccaccacagc
     5821 aacaacaaca actaccacaa caactgccac ctccaacgcc catcaacaac accaaccact
     5881 cagcaattac aacacccaat cacaacaata accacaactg caatcaaatg agaaatgtac
     5941 gcgaaattcc cattgaggtg gagcagagca aggaagccct ctcactccgc accggcgaca
     6001 tcacacagca ggcgagcaac aatgtggcgg cctccagtat tacggtgcaa agtgagaatt
     6061 tctccgcgga caagaaggcg atatcccagt cgcagcaatc gcagacgatg acctccaacg
     6121 ggatcatcag tcaggagaaa catgtatcct ccgcctcgca ggccaactac tcgatgtcgc
     6181 acaagggcgt ttccagtacc ggcagtagca tgatcaccag ctcctcgcaa atgtccgcga
     6241 tgaatggcca gatgctgaag ctcgccgatc tcaagttgga tgacctcaag tcgctgacag
     6301 ctggcagtgg gcagcaggag atcgagcaga ccatcaacaa gtattccaat atgctgacct
     6361 caatagtgag ctccctgcaa gaagatgagc gaggtggtag tgccataacc gtccacgatg
     6421 ttggtggcaa gaagtctcag tacctggaga agatcaacga agtcattcgt cgagcttggg
     6481 cagtgcccac acatggccac gagctgggct actcattgtg caattccctg cgccaaagtg
     6541 gcggcctcga tcttctcatg aagaactgcg ttaagcccga tctccagttt tcctccgcac
     6601 agcttcttga gcagtgccta accaccgaga accgcaaaca tgtggtggat aatggtctgg
     6661 ataaggtggt caatgtggcc tgtgtctgca caaagaattc caatatggag cactcccgcg
     6721 tgggcactgg catcctggag catctgttca aacactccga gggcacctgt tcggatgtga
     6781 taaggctagg tggtctggat gccgtgctct ttgagtgccg caccagtgat ttggaaactc
     6841 tgcgacactg cgctagtgca ctggcaaatt tatctctata tggtggagcc gagaatcagg
     6901 aggagatgat cctacgcaag gtgcccatgt ggttgttccc attggccttc cacaacgatg
     6961 ataacatcaa gtactatgcc tgcttggcca ttgcagtgct ggtagccaac aaggagattg
     7021 aagccgaggt gctgaagtct ggatgcttgg atttggtgga accctttgtc acctcccacg
     7081 acccctcggc ttttgcgaga tccaatctgg cacatgccca cgggcaaagc aaacattggc
     7141 tgaaaagatt ggttccagtt ttgagttcca atcgcgaaga ggcccgcaat ctggctgcct
     7201 tccatttttg catggaggcg ggcatcaaga gggagcaggg taacaccgac atcttccgcg
     7261 agatcaacgc tattgaagct ttgaaaaatg tggccagctg tccaaatgcc attgcatcca
     7321 agttcgccgc tcaggctctg agattgattg gagagacagt tccgcacaag ctgtcccaac
     7381 aggttccctt gtggtccgtg gaggatgtgc aggagtgggt aaagcaaatc ggtttcaatg
     7441 actacatcga caagttcaac gagtcccagg tggacgggga tcttttactg aagctcaatc
     7501 aggataacct ccgggctgat attggaatcg gtaatggaat acttctaaag cgttttgaac
     7561 gcgagctgca aaatctcaag cgtatggctg actactcatc caaggacacg gccaagatgc
     7621 accaatttct ctcggagatc ggtactgatt actgcaccta cacgtatgcc atgctgaatg
     7681 ctggaattga caagtgtgca ctgccgcatg tcaatgagga tatgctgatg acagagtgtg
     7741 gcatccacaa ctcgatccat cgtctgagaa ttctcaatgc ggtcaaaaac ttggagaatt
     7801 cgctgcccag ctcgtcggag gagaacatgg ccaagacgct agatgttttc gttagctata
     7861 ggcgatcaaa cggctcccaa ctagccagtc ttctcaaggt gcatctgcag cttcgtggtt
     7921 tctccgtttt catcgacgtg gagcgcctgg aggcgggcaa atttgacaac ggcctattga
     7981 acagtattcg acaggcaaag aactttgtct tagtattaac tcccgatgcc ctgcaccgct
     8041 gcattaacga cgaggactgc aaggactggg tgcatcgcga aatcgtggct gccctgaact
     8101 cgaactgcaa cattataccc atcatagacc agcaattcga ttggcccgag gtggagcgac
     8161 ttcccgagga catgcgcagt gtggcccatt ttaacggtgt taattggatc cacgactacc
     8221 aggacgcatg catcgataag ctcgaaagat ttttgcgcgg cgaaaagaat atcgatcgca
     8281 ttgcggcgat ggtgcccggc acccccggtt cggtctcata ccaaagaatg cacagcaacg
     8341 actccgacta ccagagcgga ggagcaggcg ccggatcagg agcaggaact ggaggaggag
     8401 gtggtggcgg cgttacgggc agtgtggtgg atggcctgat ggtggcggcc aatggcagcg
     8461 gacaaggtgg gccaacgagt actacatcta ctacttcttc tactcccaac tcaaattcaa
     8521 gctcaagctc taaccagagt cccccagcgg ctccagcgga ctacaaagac gatgacgaca
     8581 agtaggaatt ccggcgatgc tgatccgagc agtccgatgg ggaggaggag gaggaggagg
     8641 aggacggcga tgaggaggag gaagatgatg gatccgatga ggaaaatgga aatggaaata
     8701 gagggggcag aaatcgaccc agagacaacg gaggatgaga tgatgatccc acatagactt
     8761 gttgcattaa acgaatatta gcgcttatgt ctagaggatc tttgtgaagg aaccttactt
     8821 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     8881 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     8941 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     9001 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     9061 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     9121 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     9181 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag
     9241 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     9301 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     9361 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9421 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9481 atcatgtctg gatcggatcc gcggccgcgg ctcgagggta cctctagaga tccactagtg
     9541 tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc
     9601 tccccgggcg cgtactccac ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg
     9661 cggtagttga tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg
     9721 ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg
     9781 ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt cgacactagt tctagccagc
     9841 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     9901 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9961 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    10021 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    10081 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    10141 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    10201 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    10261 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10321 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10381 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10441 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10501 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10561 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10621 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10681 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    10741 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10801 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10861 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10921 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10981 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11041 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11101 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11161 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    11221 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11281 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11341 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11401 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11461 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11521 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11581 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11641 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11701 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11761 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11821 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
//
