LOCUS       sequence-372228-       10173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS expression of mammalian NAMPT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-mNAMPT
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(75..93)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(114..130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(127..149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    138..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(162..168,169..186,187..192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(345..362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(516..1104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(596..615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1275..2066,2067..2135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1898..1917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2136..2240)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2266..2721)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2403..2424)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2615..2634
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2848..2870
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2862..2879
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2863..2879
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2886..2905
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2886..2904
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2938..2956)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3408..3479,3881..4154,4229..4883,4945..5260,5464..5595,
                     5666..6280)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    7055..7088
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7138..7232
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7251..7489
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7436..7457
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          9272..9337
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9467..9487
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9759..9893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9796..9815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9850..9869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9960..10029
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac actagttcta
       61 gccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
      121 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      181 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      241 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      301 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      361 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      421 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1381 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1561 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1621 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1681 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1741 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1801 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1861 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1921 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1981 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2041 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2101 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2161 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2221 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     2281 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     2341 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     2401 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     2461 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     2521 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     2581 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     2641 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     2701 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     2761 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     2821 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2881 aattgtaata cgactcacta tagggcgaat tgggtacgta ccgggcccct agtatgtatg
     2941 taagttaata aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact
     3001 gcactggata tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt
     3061 tgatgtacca ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc
     3121 gctgtttgcc tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc
     3181 tatctctttc gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt
     3241 ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg
     3301 ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg
     3361 attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg
     3421 atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg
     3481 tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac
     3541 atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca
     3601 aacaaatagt gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt
     3661 ttgagatcat atcatgatca agacatctaa aggcattcat tttcgactat attctttttt
     3721 acaaaaaata taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa
     3781 agtataaacc tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat
     3841 aacgcttgta gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc
     3901 gcagagctgc attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc
     3961 cagtccgccg gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc
     4021 ctggcacaat atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct
     4081 ggtcaaccgg acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca
     4141 tttgctcaag aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa
     4201 agggtccaat taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg
     4261 gccgtgatgg gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga
     4321 tcgccgcagg gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg
     4381 gacgccaagg agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc
     4441 tccctaacgg ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg
     4501 acctatcggc agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa
     4561 tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg
     4621 aagcgtctgg cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag
     4681 cccacctccg gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg
     4741 tcgcagaagg gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag
     4801 ctctttgaca agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc
     4861 gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta
     4921 cataacatct caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc
     4981 ggacttttac gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg
     5041 gatcgccaag atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt
     5101 gttggccacc aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa
     5161 ggccacctgg ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa
     5221 ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa
     5281 caaatgatat aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc
     5341 ctgattccac acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa
     5401 gataaataaa tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg
     5461 cagatggttg ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt
     5521 gtgatgaata tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc
     5581 tttgccacga taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac
     5641 tttctaatga atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga
     5701 ggcccgaagt cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt
     5761 gccgcttttt ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact
     5821 gcgggccgga gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt
     5881 gtcaacgtcc ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc
     5941 tgtgggtccg ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg
     6001 ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga
     6061 gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc
     6121 gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc
     6181 cgatctgccg ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc
     6241 atatctggct ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa
     6301 ctgcttgttt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa
     6361 aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt
     6421 atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt
     6481 acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata
     6541 ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc
     6601 acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt
     6661 gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt
     6721 ccgagtggtt catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat
     6781 ttgctccaat atatttgtat atatttccct cacagctata tttattctaa tttaatatta
     6841 tgacttttta aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact
     6901 gaaagtgaca tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata
     6961 atagtgttgt ttatatatat caaaaataac aactataata ataagaatac atttaattta
     7021 gaaaatgctt ggatttcact ggaactaggc tagcataact tcgtataatg tatgctatac
     7081 gaagttatgc tagcggatcc aaaagctttc cggatccaag cttgcatgcc tgcaggtcgg
     7141 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     7201 actgtcctcc gagcggagta ctgtcctccg agcggagact ctagcgagcg ccggagtata
     7261 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     7321 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     7381 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     7441 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagggaatt
     7501 gggaattcat gaatgctgcg gcagaagccg agttcaacat cctgctggcc accgactcgt
     7561 acaaggttac tcactataaa caatacccac ccaacacaag caaagtttat tcctactttg
     7621 aatgccgtga aaagaagaca gaaaactcca aagtaaggaa ggtgaaatac gaggaaacag
     7681 tattttatgg gttgcagtac attcttaata agtacttaaa aggtaaagta gtgaccaaag
     7741 agaaaatcca ggaggccaaa gaagtgtaca gagaacattt ccaagatgat gtctttaacg
     7801 aaagaggatg gaactacatc cttgagaaat acgatggtca tctcccgatt gaagtaaagg
     7861 ctgttcccga gggctctgtc atccccagag ggaacgtgct gttcacagtg gaaaacacag
     7921 acccagagtg ctactggctt accaattgga ttgagactat tcttgttcag tcctggtatc
     7981 caattacagt ggccacaaat tccagagaac agaagaaaat actggccaaa tatttgttag
     8041 aaacctctgg taacttagat ggtctggaat acaagttaca tgactttggt tacagaggag
     8101 tctcttcgca agagactgct ggcatagggg catctgctca tttggttaac tttaaaggaa
     8161 cagatactgt ggcgggaatt gctctaatta aaaaatacta tgggacaaaa gatcctgttc
     8221 caggctattc tgttccagca gcagagcaca gtaccataac ggcttggggg aaagaccatg
     8281 agaaagatgc ttttgaacac atagtaacac agttctcatc agtgcctgtg tctgtggtca
     8341 gcgatagcta tgacatttat aatgcgtgtg agaaaatatg gggtgaagac ctgagacatc
     8401 tgatagtatc gagaagtaca gaggcaccac taatcatcag acctgactct ggaaatcctc
     8461 ttgacactgt attgaaggtc ttagatattt taggcaagaa gtttcctgtt actgagaact
     8521 caaaaggcta caagttgctg ccaccttatc ttagagtcat tcaaggagat ggcgtggata
     8581 tcaatacttt acaagagatt gtagagggaa tgaaacaaaa gaagtggagt atcgagaatg
     8641 tctccttcgg ttctggtggc gctttgctac agaagttaac ccgagacctc ttgaattgct
     8701 ccttcaagtg cagctatgtt gtaaccaatg gccttggggt taatgtgttt aaggacccag
     8761 ttgctgatcc caacaaaagg tcaaaaaagg gccggttatc tttacatagg acaccagcgg
     8821 ggaactttgt tacacttgaa gaaggaaaag gagaccttga ggaatatggc catgatcttc
     8881 tccatacggt tttcaagaat gggaaggtga caaaaagcta ctcatttgat gaagtcagaa
     8941 aaaatgcaca gctgaacatc gagcaggacg tggcacctca ttaggaattc cggcgatgct
     9001 gatccgagca gtccgatggg gaggaggagg aggaggagga ggacggcgat gaggaggagg
     9061 aagatgatgg atccgatgag gaaaatggaa atggaaatag agggggcaga aatcgaccca
     9121 gagacaacgg aggatgagat gatgatccca catagacttg ttgcattaaa cgaatattag
     9181 cgcttatgtc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     9241 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     9301 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     9361 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     9421 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     9481 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     9541 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     9601 gctatacaag aaaattatgg aaaaatattt gatgtatagt gccttgacta gagatcataa
     9661 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     9721 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     9781 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9841 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcggatccg
     9901 cggccgcggc tcgagggtac ctctagagat ccactagtgt cgacgatgta ggtcacggtc
     9961 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
    10021 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
    10081 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
    10141 acgggacgtg cgacggcgtc ggcgggtgcg gat
//
