LOCUS       sequence-372229-        9259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS expression of GFP::NMN-Deamidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-GFP::NMN-Deamidase
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(83..538)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(220..241)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     432..451
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     665..687
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     679..696
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     680..696
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     703..722
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        703..721
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(755..773)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1225..1296,1698..1971,2046..2700,2762..3077,3281..3412,
                     3483..4097)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4872..4905
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4938..5032
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5051..5289
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5236..5257
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             join(5313..5315,5316..5318,5319..6029)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5313..5315,5316..5318,5319..6029)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5358..5379)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5619..5638)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5966..5987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    6863..6932
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(7151..7169)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7190..7206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7190..7206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7203..7225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7214..7230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7238..7244,7245..7262,7263..7268))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7283..7304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7421..7438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7592..8180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7672..7691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8351..9142,9143..9211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8974..8993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9212..9259,1..57))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
       61 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
      121 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
      181 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
      241 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
      301 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
      361 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
      421 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
      481 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
      541 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      601 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      661 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
      721 ggcgaattgg gtacgtaccg ggcccctagt atgtatgtaa gttaataaaa cccatttttg
      781 cggaaagtag ataaaaaaaa catttttttt ttttactgca ctggatatca ttgaacttat
      841 ctgatcagtt ttaaatttac ttcgatccaa gggtatttga tgtaccaggt tctttcgatt
      901 acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc
      961 acagaaatat cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc accgtttgta
     1021 gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg tcagcggttt cgtgacgaag
     1081 ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt
     1141 cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag
     1201 gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga
     1261 ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc
     1321 ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga
     1381 actgcgaccc acaaaaatcc caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga
     1441 ttattctggt agctgttctc gctatataag acaatttttg agatcatatc atgatcaaga
     1501 catctaaagg cattcatttt cgactatatt cttttttaca aaaaatataa caaccagata
     1561 ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat
     1621 acttcggggt actttttgtt cggggttaga tgagcataac gcttgtagtt gatatttgag
     1681 atcccctatc attgcagggt gacagcggag cggcttcgca gagctgcatt aaccagggct
     1741 tcgggcaggc caaaaactac ggcacgctcc ggccacccag tccgccggag gactccggtt
     1801 cagggagcgg ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg
     1861 gggcggtcaa tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat
     1921 tctgcaacga gcgacacata ccggcgccca ggaaacattt gctcaagaac ggtgagtttc
     1981 tattcgcagt cggctgatct gtgtgaaatc ttaataaagg gtccaattac caatttgaaa
     2041 ctcagtttgc ggcgtggcct atccgggcga acttttggcc gtgatgggca gttccggtgc
     2101 cggaaagacg accctgctga atgcccttgc ctttcgatcg ccgcagggca tccaagtatc
     2161 gccatccggg atgcgactgc tcaatggcca acctgtggac gccaaggaga tgcaggccag
     2221 gtgcgcctat gtccagcagg atgacctctt tatcggctcc ctaacggcca gggaacacct
     2281 gattttccaa gccatggtgc ggatgccacg acatctgacc tatcggcagc gagtggcccg
     2341 cgtggatcag gtgatccagg agctttcgct cagcaaatgt cagcacacga tcatcggtgt
     2401 gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat tcgcctccga
     2461 ggctctaacc gatccgccgc ttctgatctg cgatgagccc acctccggac tggactcctt
     2521 taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca agaccgtcat
     2581 cctgaccatt catcagccgt cttccgagct gtttgagctc tttgacaaga tccttctgat
     2641 ggccgagggc agggtagctt tcttgggcac tcccagcgaa gccgtcgact tcttttccta
     2701 gtgagttcga tgtgtttatt aagggtatct agtattacat aacatctcaa ctcctatcca
     2761 gcgtgggtgc ccagtgtcct accaactaca atccggcgga cttttacgta caggtgttgg
     2821 ccgttgtgcc cggacgggag atcgagtccc gtgatcggat cgccaagata tgcgacaatt
     2881 ttgccattag caaagtagcc cgggatatgg agcagttgtt ggccaccaaa aatctggaga
     2941 agccactgga gcagccggag aatgggtaca cctacaaggc cacctggttc atgcagttcc
     3001 gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga accactcctc gtaaaagtgc
     3061 gacttattca gacaacggtg agtggttcca gtggaaacaa atgatataac gcttacaatt
     3121 cttggaaaca aattcgctag attttagata gaattgcctg attccacacc cttcttagtt
     3181 tttttcaatg agatgtatag tttatagttt tgcagaagat aaataaattt catttaactc
     3241 gcgaatatta atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg
     3301 cctcatcttt ttgggccaac aactcacgca agtgggtgtg atgaatatca acggagccat
     3361 cttcctcttc ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtca
     3421 tgtttagaat acatttgcat ttcaataatt tactaacttt ctaatgaatc gattcgattt
     3481 aggtgttcac ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct
     3541 gtgacacata ctttctgggc aaaacgattg ccgaattgcc gctttttctc acagtgccac
     3601 tggtcttcac ggcgattgcc tatccgatga tcggactgcg ggccggagtg ctgcacttct
     3661 tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc ggatatctaa
     3721 tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt gggtccgccg gttatcatac
     3781 cattcctgct ctttggcggc ttcttcttga actcgggctc ggtgccagta tacctcaaat
     3841 ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt aaccaatggg
     3901 cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc cccagttcgg
     3961 gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg gactacgtgg
     4021 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     4081 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt ttttttacca
     4141 ttattaccat cgtgtttact gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt
     4201 gccaataaaa acaagatatg acctatagaa tacaagtatt tccccttcga acatccccac
     4261 aagtagactt tggatttgtc ttctaaccaa aagacttaca cacctgcata ccttacatca
     4321 aaaactcgtt tatcgctaca taaaacaccg ggatatattt tttatataca tacttttcaa
     4381 atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg tttcgtagtt gctctttcgc
     4441 tgtctcccac ccgctctccg caacacattc accttttgtt cgacgacctt ggagcgactg
     4501 tcgttagttc cgcgcgattc ggttcgctca aatggttccg agtggttcat ttcgtctcaa
     4561 tagaaattag taataaatat ttgtatgtac aatttatttg ctccaatata tttgtatata
     4621 tttccctcac agctatattt attctaattt aatattatga ctttttaagg taattttttg
     4681 tgacctgttc ggagtgatta gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt
     4741 ccttgtgtag atgcatctca aaaaaatggt gggcataata gtgttgttta tatatatcaa
     4801 aaataacaac tataataata agaatacatt taatttagaa aatgcttgga tttcactgga
     4861 actaggctag cataacttcg tataatgtat gctatacgaa gttatgctag cggatccaag
     4921 cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     4981 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact
     5041 ctagcgagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa
     5101 acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac
     5161 aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa
     5221 ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag
     5281 agaactctga atagggaatt gggaattcca acatggtgag caagggcgag gagctgttca
     5341 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     5401 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     5461 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     5521 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     5581 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     5641 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5701 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     5761 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     5821 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5881 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     5941 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     6001 tcactctcgg catggacgag ctgtacaagt ccggactcag atctactgac agtgaactga
     6061 tgcagttaag tgaacaggtt gggcaggcgc tgaaagcccg tggcgcaacc gtaacaactg
     6121 ccgagtcttg taccggtggt tgggtagcga aagtgattac cgatattgcc ggtagctccg
     6181 cctggtttga acgcggattt gtcacctaca gtaacgaagc caaagcgcag atgatcggcg
     6241 tacgcgaaga gacgctggcg cagcatggcg cggtgagtga acccgtcgtg gtggaaatgg
     6301 cgataggcgc actgaaagcg gctcgtgctg attatgccgt gtctattagt ggtatcgccg
     6361 ggccggatgg cggcagtgaa gagaagcctg tcggcaccgt ctggtttgct tttgccactg
     6421 cccgcggtga aggcattacc cggcgggaat gcttcagcgg cgaccgtgat gcggtgcgtc
     6481 gtcaggctac tgcgtatgca ttgcagacct tgtggcaaca atttctacaa aacacttgag
     6541 acgtctatcg ataccgtcga ctaaagccaa atagaaaatt attcagttcc tggcttaagt
     6601 ttttaaaagt gatattattt atttggttgt aaccaaccaa aagaatgtaa ataactaata
     6661 cataattatg ttagttttaa gttagcaaca aattgatttt agctatatta gctacttggt
     6721 taataaatag aatatattta tttaaagata attgcgtttt tattgtcagg gagtgagttt
     6781 gcttaaaaac tcgtttagat ccgcggccgc ggctcgaggg tacctctaga gatccactag
     6841 tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg
     6901 gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt
     6961 ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc
     7021 tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc
     7081 ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca
     7141 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     7201 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7261 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7321 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7381 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7441 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8401 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9241 tttatcaggg ttattgtct
//
