LOCUS       sequence-372234-        7278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Opto-T7RNAP*(69), equal expr..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Baumschlager A, Aoki SK, Khammash M
  TITLE     Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for 
            Precise Spatiotemporal Gene Expression Control.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 
            10.1021/acssynbio.7b00169. Epub 2017 Oct 18.
  PUBMED    29045151
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00169"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2157-2167"
COMMENT     Sequence Label: pAB171
FEATURES             Location/Qualifiers
     source          1..7278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             331..780
                     /codon_start=1
                     /product="""negative Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=nMagHigh1
                     /note="rapidly heterodimerizes with pMag or pMagFast2 upon 
                     blue light irradiation"
                     /translation="HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTIRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSMGFQCETE"
     terminator      796..882
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1478..1927
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     terminator      4399..4485
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4666..5616)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5664..5886
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6378..6472)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6493..7152)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7067..7086
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7153..7255)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtctagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
       61 tctctactgt ttctccatac ccgttttttt ggaaggaggt aaagatctat gaacacgatt
      121 aacatcgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg
      181 gctgaccatt acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac
      241 gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt
      301 gcggataacg ctgccggcgg ttctggaggt cacactcttt acgcccctgg aggatacgac
      361 attatgggat atttggatca gattgggaac cgcccaaacc ctcaggtcga actggggcct
      421 gtggacacgt catgtgccct gatcctgtgc gatctgaagc aaaaggacac tccgatcgtc
      481 tacgcctcgg aagccttctt gtatatgacc ggatacagca atgcagaggt gctcggcagg
      541 aactgcagat tcctgcagtc ccccgacggg atggtgaaac caaagtcgac tcgcaaatat
      601 gtggactcga acacgatcaa caccatccgg aaggccatcg accggaacgc cgaggtccag
      661 gtggaggtgg tcaactttaa gaagaacggc cagcggttcg tgaactttct gaccatcatt
      721 ccggtccggg atgaaaccgg agagtacaga tactccatgg gattccagtg cgaaaccgaa
      781 taacctagga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      841 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag
      901 ctgcagctag ggtacgggtt ttgctgcccg caaacgggct gttctggtgt tgctagtttg
      961 ttatcagaat cgcagatccg gcttcagccg gtttgccggc tgaaagcgct atttcttcca
     1021 gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg tcggcagctt
     1081 tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt gagctgtaac
     1141 aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg tttcacctgt
     1201 tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat ggtgaacagc
     1261 tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc
     1321 atctgtgcat gcgccgatca acgtctcatt ttcgccagat atcggcgcgc cgcttcgtag
     1381 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg
     1441 tttctccata cccgtttttt tggaaggagg taaaatgcat actctttacg cccctggagg
     1501 atacgacatt atgggatatt tgcggcagat taggaaccgc ccaaaccctc aggtcgaact
     1561 ggggcctgtg gacacgtcat gtgccctgat cctgtgcgat ctgaagcaaa aggacactcc
     1621 gatcgtctac gcctcggaag ccttcttgta tatgaccgga tacagcaatg cagaggtgct
     1681 cggcaggaac tgcagattcc tgcagtcccc cgacgggatg gtgaaaccaa agtcgactcg
     1741 caaatatgtg gactcgaaca cgatcaacac catgcggaag gccatcgacc ggaacgccga
     1801 ggtccaggtg gaggtggtca actttaagaa gaacggccag cggttcgtga actttctgac
     1861 catgattccg gtccgggatg aaaccggaga gtacagatac tccatgggat tccagtgcga
     1921 aaccgaaggc ggttctggag gtgccaagcc tctcatcact accctactcc ctaagatgat
     1981 tgcacgcatc aacgactggt ttgaggaagt gaaagctaag cgcggcaagc gcccgacagc
     2041 cttccagttc ctgcaagaaa tcaagccgga agccgtagcg tacatcacca ttaagaccac
     2101 tctggcttgc ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg
     2161 tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt
     2221 caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca agaaagcatt
     2281 tatgcaagtt gtcgaggctg acatgctctc taagggtcta ctcggtggcg aggcgtggtc
     2341 ttcgtggcat aaggaagact ctattcatgt aggagtacgc tgcatcgaga tgctcattga
     2401 gtcaaccgga atggttagct tacaccgcca aaatgctggc gtagtaggtc aagactctga
     2461 gactatcgaa ctcgcacctg aatacgctga ggctatcgca acccgtgcag gtgcgctggc
     2521 tggcatctct ccgatgttcc aaccttgcgt agttcctcct aagccgtgga ctggcattac
     2581 tggtggtggc tattgggcta acggtcgtcg tcctctggcg ctggtgcgta ctcacagtaa
     2641 gaaagcactg atgcgctacg aagacgttta catgcctgag gtgtacaaag cgattaacat
     2701 tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta gcggtcgcca acgtaatcac
     2761 caagtggaag cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag aactcccgat
     2821 gaaaccggaa gacatcgaca tgaatcctga ggctctcacc gcgtggaaac gtgctgccgc
     2881 tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg agttcatgct
     2941 tgagcaagcc aataagtttg ctaaccataa ggccatctgg ttcccttaca acatggactg
     3001 gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata tgaccaaagg
     3061 actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa ggttactact ggctgaaaat
     3121 ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca tcaagttcat
     3181 tgaggaaaac cacgagaaca tcatggcttg cgctaagtct ccactggaga acacttggtg
     3241 ggctgagcaa gattctccgt tctgcttcct tgcgttctgc tttgagtacg ctggggtaca
     3301 gcaccacggc ctgagctata actgctccct tccgctggcg tttgacgggt cttgctctgg
     3361 catccagcac ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg ttaacttgct
     3421 tcctagtgaa accgttcagg acatctacgg gattgttgct aagaaagtca acgagattct
     3481 acaagcagac gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg atgagaacac
     3541 tggtgaaatc tctgagaaag tcaagctggg cactaaggca ctggctggtc aatggctggc
     3601 ttacggtgtt actcgcagtg tgactaagag ttcagtcatg acgctggctt acgggtccaa
     3661 agagttcggc ttccgtcaac aagtgctgga agataccatt cagccagcta ttgattccgg
     3721 caagggtctg atgttcactc agccgaatca ggctgctgga tacatggcta agctgatttg
     3781 ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca atgaactggc ttaagtctgc
     3841 tgctaagctg ctggctgctg aggtcaaaga taagaagact ggagagattc ttcgcaagcg
     3901 ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg tggcaggaat acaagaagcc
     3961 tattcagacg cgcttgaacc tgatgttcct cggtcagttc cgcttacagc ctaccattaa
     4021 caccaacaaa gatagcgaga ttgatgcaca caaacaggag tctggtatcg ctcctaactt
     4081 tgtacacagc caagacggta gccaccttcg taagactgta gtgtgggcac acgagaagta
     4141 cggaatcgaa tcttttgcac tgattcacga ctccttcggt accattccgg ctgacgctgc
     4201 gaacctgttc aaagcagtgc gcgaaactat ggttgacaca tatgagtctt gtgatgtact
     4261 ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg acaaaatgcc
     4321 agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg acttcgcgtt
     4381 cgcgtaatct agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     4441 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagacc
     4501 tagctgcagc tagggtacgg gttttgctgc ccgcaaacgg gctgttctgg tgttgctagg
     4561 atccatatgg acagttttcc ctttgatatg taacggtgaa cagttgttct acttttgttt
     4621 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta
     4681 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc
     4741 attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg
     4801 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc
     4861 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc
     4921 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg
     4981 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg
     5041 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat
     5101 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag
     5161 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg
     5221 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact
     5281 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc
     5341 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc
     5401 tttagctaat acaccataag cattttccct actgatgttc atcatctgag cgtattggtt
     5461 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag
     5521 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc
     5581 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt
     5641 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg
     5701 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag
     5761 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat
     5821 aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta
     5881 taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc
     5941 tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg
     6001 caaatcgctg aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc
     6061 gtgacattca gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca
     6121 ggcgcctttt atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg
     6181 gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc
     6241 agcagttcct gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca
     6301 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta
     6361 ctgtccctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     6421 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     6481 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     6541 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     6601 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     6661 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     6721 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     6781 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     6841 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     6901 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     6961 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     7021 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     7081 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     7141 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     7201 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     7261 tcattttcgc cagatatc
//
