LOCUS       sequence-37224-e        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of blue-green emission ratiometric genetically 
            encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-GEM-GECO1
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     285..306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(670..689)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt
       61 cggctgagct caccagagaa cgtgtatata aaggccgacg agcagaagaa cggcatcaag
      121 gcgtacttca agatccgcca caacatcgag ggcggcggcg tgcagctcgc ctaccactac
      181 cagcagatca cccccatagg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      241 gtgcagtcca tactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      301 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg tggcagcggt
      361 ggcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcca ggtcgagctg
      421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
      481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      541 accctcgtga ccaccctgtc ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      601 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
      661 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      721 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      781 cacaagctgg agtacagcac gcgtgaccaa ctgactgaag agcagatcgc agaatttaaa
      841 gaggctttct ccctatttga caaggacggg gatgggacga taacaaccaa ggagctgggg
      901 acggtgatgc ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat
      961 gaagtagatg ccgacggtga cggcacaatc gacttccctg agttcctgac aatgatggca
     1021 cctaaaatgc aggacacaga cagtgaagaa gaaattagag aagcgttccg tgtgtttgat
     1081 aaggacggca atggctacat cggcgcagca gagcttcgcc acgtgatgac aaaccttgga
     1141 gagaagttaa cagatgaaga ggttgatgaa atgatcaggg tagcagacat cgatggggat
     1201 ggtcaggtaa actacgaaga gtttgtacaa atgatgacag cgaagtag
//
