LOCUS       sequence-372244-        7278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Opto-T7RNAP*(563-F1), equal expr..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Baumschlager A, Aoki SK, Khammash M
  TITLE     Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for 
            Precise Spatiotemporal Gene Expression Control.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 
            10.1021/acssynbio.7b00169. Epub 2017 Oct 18.
  PUBMED    29045151
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00169"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2157-2167"
COMMENT     Sequence Label: pAB168
FEATURES             Location/Qualifiers
     source          1..7278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1813..2262
                     /codon_start=1
                     /product="""negative Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=nMagHigh1
                     /note="rapidly heterodimerizes with pMag or pMagFast2 upon 
                     blue light irradiation"
                     /translation="HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTIRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSMGFQCETE"
     terminator      2278..2364
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             2960..3409
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMagFast2
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with fast switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALILCDLKQK
                     DTPVVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     terminator      4399..4485
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4666..5616)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5664..5886
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6378..6472)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6493..7152)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7067..7086
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7153..7255)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtctagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
       61 tctctactgt ttctccatac ccgttttttt ggaaggaggt aaagatctat gaacacgatt
      121 aacatcgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg
      181 gctgaccatt acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac
      241 gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt
      301 gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc
      361 atcaacgact ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag
      421 ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct
      481 tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc
      541 attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa
      601 aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa
      661 gttgtcgagg ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg
      721 cataaggaag actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc
      781 ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc
      841 gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct ggctggcatc
      901 tctccgatgt tccaaccttg cgtagttcct cctaagccgt ggactggcat tactggtggt
      961 ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag taagaaagca
     1021 ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa
     1081 aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg
     1141 aagcattgtc cggtcgagga catccctgcg attgagcgtg aagaactccc gatgaaaccg
     1201 gaagacatcg acatgaatcc tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg
     1261 taccgcaagg acaaggctcg caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa
     1321 gccaataagt ttgctaacca taaggccatc tggttccctt acaacatgga ctggcgcggt
     1381 cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa aggactgctt
     1441 acgctggcga aaggtaaacc aatcggtaag gaaggttact actggctgaa aatccacggt
     1501 gcaaactgtg cgggtgtcga taaggttccg ttccctgagc gcatcaagtt cattgaggaa
     1561 aaccacgaga acatcatggc ttgcgctaag tctccactgg agaacacttg gtgggctgag
     1621 caagattctc cgttctgctt ccttgcgttc tgctttgagt acgctggggt acagcaccac
     1681 ggcctgagct ataactgctc ccttccgctg gcgtttgacg ggtcttgctc tggcatccag
     1741 cacttctccg cgatgctccg agatgaggta ggtggtcgcg cggttaactt gcttcctggc
     1801 ggttctggag gtcacactct ttacgcccct ggaggatacg acattatggg atatttggat
     1861 cagattggga accgcccaaa ccctcaggtc gaactggggc ctgtggacac gtcatgtgcc
     1921 ctgatcctgt gcgatctgaa gcaaaaggac actccgatcg tctacgcctc ggaagccttc
     1981 ttgtatatga ccggatacag caatgcagag gtgctcggca ggaactgcag attcctgcag
     2041 tcccccgacg ggatggtgaa accaaagtcg actcgcaaat atgtggactc gaacacgatc
     2101 aacaccatcc ggaaggccat cgaccggaac gccgaggtcc aggtggaggt ggtcaacttt
     2161 aagaagaacg gccagcggtt cgtgaacttt ctgaccatca ttccggtccg ggatgaaacc
     2221 ggagagtaca gatactccat gggattccag tgcgaaaccg aataacctag gaggcatcaa
     2281 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2341 aacgctctcc tgagtaggac aaatccgccg ccctagacct agctgcagct agggtacggg
     2401 ttttgctgcc cgcaaacggg ctgttctggt gttgctagtt tgttatcaga atcgcagatc
     2461 cggcttcagc cggtttgccg gctgaaagcg ctatttcttc cagaattgcc atgatttttt
     2521 ccccacggga ggcgtcactg gctcccgtgt tgtcggcagc tttgattcga taagcagcat
     2581 cgcctgtttc aggctgtcta tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc
     2641 aatttcatgt tctagttgct ttgttttact ggtttcacct gttctattag gtgttacatg
     2701 ctgttcatct gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac
     2761 tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atgcgccgat
     2821 caacgtctca ttttcgccag atatcggcgc gccgcttcgt agcattttta tccataagat
     2881 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
     2941 tttggaagga ggtaaaatgc atactcttta cgcccctgga ggatacgaca ttatgggata
     3001 tttgcggcag attaggaacc gcccaaaccc tcaggtcgaa ctggggcctg tggacacgtc
     3061 atgtgccctg atcctgtgcg atctgaagca aaaggacact ccggtggtct acgcctcgga
     3121 agccttcttg tatatgaccg gatacagcaa tgcagaggtg ctcggcagga actgcagatt
     3181 cctgcagtcc cccgacggga tggtgaaacc aaagtcgact cgcaaatatg tggactcgaa
     3241 cacgatcaac accatgcgga aggccatcga ccggaacgcc gaggtccagg tggaggtggt
     3301 caactttaag aagaacggcc agcggttcgt gaactttctg accatgattc cggtccggga
     3361 tgaaaccgga gagtacagat actccatggg attccagtgc gaaaccgaag gcggttctgg
     3421 aggtagtgaa accgttcagg acatctacgg gattgttgct aagaaagtca acgagattct
     3481 acaagcagac gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg atgagaacac
     3541 tggtgaaatc tctgagaaag tcaagctggg cactaaggca ctggctggtc aatggctggc
     3601 ttacggtgtt actcgcagtg tgactaagag ttcagtcatg acgctggctt acgggtccaa
     3661 agagttcggc ttccgtcaac aagtgctgga agataccatt cagccagcta ttgattccgg
     3721 caagggtctg atgttcactc agccgaatca ggctgctgga tacatggcta agctgatttg
     3781 ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca atgaactggc ttaagtctgc
     3841 tgctaagctg ctggctgctg aggtcaaaga taagaagact ggagagattc ttcgcaagcg
     3901 ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg tggcaggaat acaagaagcc
     3961 tattcagacg cgcttgaacc tgatgttcct cggtcagttc cgcttacagc ctaccattaa
     4021 caccaacaaa gatagcgaga ttgatgcaca caaacaggag tctggtatcg ctcctaactt
     4081 tgtacacagc caagacggta gccaccttcg taagactgta gtgtgggcac acgagaagta
     4141 cggaatcgaa tcttttgcac tgattcacga ctccttcggt accattccgg ctgacgctgc
     4201 gaacctgttc aaagcagtgc gcgaaactat ggttgacaca tatgagtctt gtgatgtact
     4261 ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg acaaaatgcc
     4321 agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg acttcgcgtt
     4381 cgcgtaatct agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     4441 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagacc
     4501 tagctgcagc tagggtacgg gttttgctgc ccgcaaacgg gctgttctgg tgttgctagg
     4561 atccatatgg acagttttcc ctttgatatg taacggtgaa cagttgttct acttttgttt
     4621 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta
     4681 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc
     4741 attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg
     4801 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc
     4861 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc
     4921 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg
     4981 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg
     5041 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat
     5101 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag
     5161 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg
     5221 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact
     5281 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc
     5341 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc
     5401 tttagctaat acaccataag cattttccct actgatgttc atcatctgag cgtattggtt
     5461 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag
     5521 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc
     5581 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt
     5641 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg
     5701 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag
     5761 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat
     5821 aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta
     5881 taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc
     5941 tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg
     6001 caaatcgctg aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc
     6061 gtgacattca gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca
     6121 ggcgcctttt atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg
     6181 gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc
     6241 agcagttcct gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca
     6301 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta
     6361 ctgtccctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     6421 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     6481 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     6541 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     6601 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     6661 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     6721 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     6781 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     6841 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     6901 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     6961 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     7021 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     7081 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     7141 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     7201 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     7261 tcattttcgc cagatatc
//
