LOCUS       sequence-37225-e        1251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of green excitation ratiometric genetically 
            encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-GEX-GECO1
FEATURES             Location/Qualifiers
     source          1..1251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     285..306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(673..692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             808..1251
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAELKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDLPEFQTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt
       61 cggctgagct cactcgagaa cgtctatatc aaggccgacg agcagaagaa cggcatcaag
      121 gcgaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac
      181 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      241 gtgcagtcca tactttcgaa agaccccaac gagaagcgtg atcacatggt cctgctggag
      301 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga
      361 gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat ccaggtcgag
      421 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc
      481 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      541 cccaccctcg tgaccaccct gtcctatggc gtgcagtgct tcagccgcta ccccgaccac
      601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc
      661 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      721 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      781 gggcacaagc tggagtacaa cacgcgtgac caactgactg aagagcagat cgcagaactt
      841 aaagaggctt tctccctatt tgacaaggac ggggatggga cgataacaac caaggagctg
      901 gggacggtga tgcggtctct ggggcagaac cccacagaag cagagctgca ggacatgatc
      961 aatgaagtag atgccgacgg tgacggcaca atcgacctcc ctgagttcca gacaatgatg
     1021 gcaagaaaaa tgaatgacac agacagtgaa gaggaaatta gagaagcgtt ccgtgtgttt
     1081 gataaggacg gcaatggcta catcggcgca gcagagcttc gccacgtgat gacaaacctt
     1141 ggagagaagt taacagatga agaggtagat gaaatgatca gggtagcaga catcgatggg
     1201 gatggtcagg taaactacga agagtttgta caaatgatga cagcgaagta g
//
