LOCUS       sequence-372255-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged human CAD A1400E in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD A1400E
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1921..1930
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8598..8621
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(8687..9261,9262..9264,9265..9273)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8854..8871)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9081..9100
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9274..9669
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             9840..10309
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(10439..10456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10610..11198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10690..10709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11362..12153,12154..12222))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11985..12004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12223..12327)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccatggcggc cctagtgttg gaggacgggt cggtcctgcg gggccagccc tttggggccg
     1981 ccgtgtcgac tgccggggaa gtggtgtttc aaaccggcat ggtcggctac cccgaggccc
     2041 tcactgatcc ctcctacaag gcacagatct tagtgctcac ctatcctctg atcggcaact
     2101 atggcatccc cccagatgaa atggatgagt tcggtctctg caagtggttt gaatcctcgg
     2161 gcatccacgt agcagcactg gtagtgggag agtgctgtcc tactcccagc cactggagtg
     2221 ccacccgcac cctgcatgag tggctgcagc agcatggcat ccctggcttg caaggagtag
     2281 acactcggga gctgaccaag aagttgcggg aacaggggtc tctgctgggg aagctggtcc
     2341 agaatggaac agaaccttca agtctgccat tcttggaccc caatgcccgc cccctggtac
     2401 cagaggtctc cattaagact ccacgggtat tcaatacagg gggtgcccct cggatccttg
     2461 ctttggactg tggcctcaag tataatcaga tccgatgcct ctgccagcgt ggggctgagg
     2521 tcactgtggt accctgggac catgcactag acagccaaga gtatgagggt ctcttcttaa
     2581 gtaatgggcc tggtgaccct gcctcctatc ccagtgtcgt atccacactg agccgtgttt
     2641 tatctgagcc taatccccga cctgtctttg ggatctgcct gggacaccag ctattggcct
     2701 tagccattgg ggccaagact tacaagatga gatatgggaa ccgaggccat aaccagccct
     2761 gcttgttggt gggctctggg cgctgctttc tgacatccca gaaccatggg tttgctgtgg
     2821 agacagactc actgccagca gactgggctc ctctcttcac caacgccaat gatggttcca
     2881 atgaaggcat tgtgcacaac agcttgcctt tcttcagtgt ccagtttcac ccagagcacc
     2941 aagctggccc ttcagatatg gaactgcttt tcgatatctt tctggaaact gtgaaagagg
     3001 ccacagctgg gaaccctggg ggccagacag ttagagagcg gctgactgag cgcctctgtc
     3061 cccctgggat tcccactccc ggctctggac ttccaccacc acgaaaggtt ctgatcctgg
     3121 gctcaggggg cctctccatt ggccaagctg gagaatttga ctactcgggc tctcaggcaa
     3181 ttaaggccct gaaggaggaa aacatccaga cgttgctgat caaccccaat attgccacag
     3241 tgcagacctc ccaggggctg gccgacaagg tctattttct tcccataaca cctcattatg
     3301 taacccaggt gatacgtaat gaacgccccg atggtgtgtt actgactttt gggggccaga
     3361 ctgctctgaa ctgtggtgtg gagctgacca aggccggggt gctggctcgg tatggggtcc
     3421 gggtcctggg cacaccagtg gagaccattg agctgaccga ggatcgacgg gcctttgctg
     3481 ccagaatggc agagatcgga gagcatgtgg ccccgagcga ggcagcaaat tctcttgaac
     3541 aggcccaggc agccgctgaa cggctggggt accctgtgct agtgcgtgca gcctttgccc
     3601 tgggtggcct gggctctggc tttgcctcta acagggagga gctctctgct ctcgtggccc
     3661 cagcttttgc ccataccagc caagtgctag tagacaagtc tctgaaggga tggaaggaga
     3721 ttgagtacga ggtggtgaga gacgcctatg gcaactgtgt cacggtgtgt aacatggaga
     3781 acttggaccc actgggcatc cacactggtg agtccatagt ggtggcccct agccagacac
     3841 tgaatgacag ggagtatcag ctcctgaggc agacagctat caaggtgacc cagcacctgg
     3901 gaattgttgg ggagtgcaat gtgcagtatg ccttgaaccc tgagtctgag cagtattaca
     3961 tcattgaagt gaatgccagg ctctctcgca gctctgccct ggccagtaag gccacaggtt
     4021 atccactggc ttatgtggca gccaagctag cattgggcat ccctttgcct gagctcagga
     4081 actctgtgac agggggtaca gcagcctttg aacccagcgt ggattattgt gtggtgaaga
     4141 ttcctcgatg ggaccttagc aagttcctgc gagtcagcac aaagattggg agctgcatga
     4201 agagcgttgg tgaagtcatg ggcattgggc gttcatttga ggaggccttc cagaaggccc
     4261 tgcgcatggt ggatgagaac tgtgtgggct ttgatcacac agtgaaacca gtcagcgata
     4321 tggagttgga gactccaaca gataagcgga tttttgtggt ggcagctgct ttgtgggctg
     4381 gttattcagt ggaccgcctg tatgagctca cacgcatcga ccgctggttc ctgcaccgaa
     4441 tgaagcgtat catcgcacat gcccagctgc tagaacaaca ccgtggacag cctttgccgc
     4501 cagacctgct gcaacaggcc aagtgtcttg gcttctcaga caaacagatt gcccttgcag
     4561 ttctgagcac agagctggct gttcgcaagc tgcgtcagga actggggatc tgtccagcag
     4621 tgaaacagat tgacacagtt gcagctgagt ggccagccca gacaaattac ctatacctaa
     4681 cgtattgggg caccacccat gacctcacct ttcgaacacc tcatgtccta gtccttggct
     4741 ctggcgtcta ccgtattggc tctagcgttg aatttgactg gtgtgctgta ggctgcatcc
     4801 agcagctccg aaagatggga tataagacca tcatggtgaa ctataaccca gagacagtca
     4861 gcaccgacta tgacatgtgt gatcgactct actttgatga gatctctttt gaggtggtga
     4921 tggacatcta tgagctcgag aaccctgaag gtgtgatcct atccatgggt ggacagctgc
     4981 ccaacaacat ggccatggcg ttgcatcggc agcagtgccg ggtgctgggc acctcccctg
     5041 aagccattga ctcggctgag aaccgtttca agttttcccg gctccttgac accattggta
     5101 tcagccagcc tcagtggagg gagctcagtg acctcgagtc tgctcgccaa ttctgccaga
     5161 ccgtggggta cccctgtgtg gtgcgcccct cctatgtgct gagcggtgct gctatgaatg
     5221 tggcctacac ggatggagac ctggagcgct tcctgagcag cgcagcagcc gtctccaaag
     5281 agcatcccgt ggtcatctcc aagttcatcc aggaggctaa ggagattgac gtggatgccg
     5341 tggcctctga tggtgtggtg gcagccatcg ccatctctga gcatgtggag aatgcaggtg
     5401 tgcattcagg tgatgcgacg ctggtgaccc ccccacaaga tatcactgcc aaaaccctgg
     5461 agcggatcaa agccattgtg catgctgtgg gccaggagct acaggtcaca ggacccttca
     5521 atctgcagct cattgccaag gatgaccagc tgaaagttat tgaatgcaac gtacgtgtct
     5581 ctcgctcctt ccccttcgtt tccaagacac tgggtgtgga cctagtagcc ttggccacgc
     5641 gggtcatcat gggggaagaa gtggaacctg tggggctaat gactggttct ggagttgtgg
     5701 gagtaaaggt gcctcagttc tccttctccc gcttggcggg tgctgacgtg gtgttgggtg
     5761 tggaaatgac cagtactggg gaggtggccg gctttgggga gagccgctgt gaggcatacc
     5821 tcaaggccat gctaagcact ggctttaaga tccccaagaa gaatatcctg ctgaccattg
     5881 gcagctataa gaacaaaagc gagctgctcc caactgtgcg gctactggag agcctgggct
     5941 acagcctcta tgccagtctc ggcacagctg acttctacac tgagcatggc gtcaaggtaa
     6001 cagctgtgga ctggcacttt gaggaggctg tggatggtga gtgcccacca cagcggagca
     6061 tcctggagca gctagctgag aaaaactttg agctggtgat taacctgtca atgcgtggag
     6121 aagggggccg gcgtctctct tcctttgtca ccaagggcta ccgcacccga cgcttggccg
     6181 ctgacttctc cgtgccccta atcatcgata tcaagtgcac caaactcttt gtggaggccc
     6241 taggccagat cgggccagcc cctcctttga aggtgcatgt tgactgtatg acctcccaaa
     6301 agcttgtgcg actgccggga ttgattgatg tccatgtgca cctgcgggaa ccaggtggga
     6361 cacataagga ggactttgct tcaggcacag ccgctgccct ggctgggggt atcaccatgg
     6421 tgtgtgccat gcctaatacc cggcccccca tcattgacgc ccctgctctg gccctggccc
     6481 agaagctggc agaggctggc gcccggtgcg actttgcgct attccttggg gcctcgtctg
     6541 aaaatgcagg aaccttgggc accgtggccg ggtctgcagc cgggctgaag ctttacctca
     6601 atgagacctt ctctgagctg cggctggaca gcgtggtcca gtggatggag catttcgaga
     6661 catggccctc ccacctcccc attgtggctc acgcggagca gcaaaccgtg gctgctgtcc
     6721 tcatggtggc tcagctcact cagcgctcag tgcacatatg tcacgtggca cggaaggagg
     6781 agatcctgct aattaaagct gcaaaggcac ggggcttgcc agtgacctgc gaggtggctc
     6841 cccaccacct gttcctaagc catgatgacc tggagcgcct ggggcctggg aagggggagg
     6901 tccggcctga gcttggctcc cgccaggatg tggaagccct gtgggagaac atggctgtca
     6961 tcgactgctt tgcctcagac catgctcccc ataccttgga ggagaagtgt gggtccaggc
     7021 ccccacctgg gttcccaggg ttagagacca tgctgccact actcctgacg gctgtaagcg
     7081 agggccggct cagcctggac gacctgctgc agcgattgca ccacaatcct cggcgcatct
     7141 ttcacctgcc tccgcaggag gacacctatg tggaggtgga tctggagcat gagtggacaa
     7201 ttcccagcca catgcccttc tccaaggccc actggacacc ttttgaaggg cagaaagtga
     7261 agggcaccgt ccgccgtgtg gtcctgcgag gggaggttgc ctatatcgat gggcaggttc
     7321 tggtaccccc gggctatgga caggatgtac ggaagtggcc acagggggct gttcctcagc
     7381 tcccaccctc agcccctgcc actagtgaga tgaccacgac acctgaaaga ccccgccgtg
     7441 gcatcccagg gcttcctgat ggccgcttcc atctgccgcc ccgaatccat cgagcctccg
     7501 acccaggttt gccagctgag gagccaaagg agaagtcctc tcggaaggta gccgagccag
     7561 agctgatggg aacccctgat ggcacctgct accctccacc accagtaccg agacaggcat
     7621 ctccccagaa cctggggacc cctggcttgc tgcaccccca gacctcaccc ctgctgcact
     7681 cattagtggg ccaacatatc ctgtccgtcc agcagttcac caaggatcag atgtctcacc
     7741 tgttcaatgt ggcacacaca ctgcgtatga tggtgcagaa ggagcggagc ctcgacatcc
     7801 tgaaggggaa ggtcatggcc tccatgttct atgaagtgag cacacggacc agcagctcct
     7861 ttgcagcagc catggcccgg ctgggaggtg ctgtgctcag cttctcggaa gccacatcgt
     7921 ccgtccagaa gggcgaatcc ctggctgact ccgtgcagac catgagctgc tatgccgacg
     7981 tcgtcgtgct ccggcacccc cagcctggag cagtggagct ggccgccaag cactgccgga
     8041 ggccagtgat caatgctggg gatggggtcg gagagcaccc cacccaggcc ctgctggaca
     8101 tcttcaccat ccgtgaggag ctgggaactg tcaatggcat gacgatcacg atggtgggtg
     8161 acctgaagca cggacgcaca gtacattccc tggcctgcct gctcacccag tatcgtgtca
     8221 gcctgcgcta cgtggcacct cccagcctgc gcatgccacc cactgtgcgg gccttcgtgg
     8281 cctcccgcgg caccaagcag gaggaattcg agagcattga ggaggcgctg cctgacactg
     8341 atgtgctcta catgactcga atccagaagg aacgatttgg ctctacccag gagtacgaag
     8401 cttgctttgg tcagttcatc ctcactcccc acatcatgac ccgggccaag aagaagatgg
     8461 tggtgatgca cccgatgccc cgtgtcaacg agataagcgt ggaagtggac tcggatcccc
     8521 gcgcagccta cttccgccag gctgagaacg gcatgtacat ccgcatggct ctgttagcca
     8581 ccgtgctggg ccgtttcgac tacaaagacg atgacgacaa gtaggaattc ctgcaggcct
     8641 cgagggccgg cgcgccgcgg ccgctacgta aattccgccc cccccccccc tctccctccc
     8701 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     8761 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     8821 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     8881 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     8941 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     9001 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     9061 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     9121 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     9181 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     9241 tttgaaaaac acgatgataa tatggccaca accatggttc ctttgtctca agaagaatcc
     9301 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     9361 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     9421 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     9481 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     9541 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     9601 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     9661 gagggctaag tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     9721 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     9781 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     9841 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     9901 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     9961 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
    10021 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
    10081 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
    10141 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
    10201 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
    10261 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
    10321 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt
    10381 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    10441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    11041 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    11221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11281 caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata
    11341 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11401 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11461 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11521 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11581 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11641 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11701 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11761 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11821 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11881 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11941 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12001 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12061 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12121 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12181 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12241 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    12301 gaaaaataaa caaatagggg ttccgcgcac atttc
//
