LOCUS       sequence-37226-e        1254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Red fluorescent genetically encoded Ca2+ 
            indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-R-GECO1
FEATURES             Location/Qualifiers
     source          1..1254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     768..787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             811..1254
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 atggtcgact cttcacgtcg taagtggaat aaggcaggtc acgcagtcag agctataggt
       61 cggctgagct cacccgtggt ttccgagcgg atgtaccccg aggacggcgc cctgaagagc
      121 gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaagacc
      181 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
      241 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgagggc
      301 cgccactcca ccggcggcat ggacgagctg tacaagggag gtacaggcgg gagtctggtg
      361 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      421 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      481 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      541 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacattaa gcacccagcc
      601 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
      661 aacttcgagg acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgta
      721 ttcatctaca aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      781 aagaagacca tgggctggga ggctacgcgt gaccaactga ctgaagagca gatcgcagaa
      841 tttaaagagg ctttctccct atttgacaag gacggggatg ggacgataac aaccaaggag
      901 ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      961 atcaatgaag tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg
     1021 atggcaagaa aaatgaatga cacagacagt gaagaggaaa ttagagaagc gttccgcgtg
     1081 tttgataagg acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac
     1141 cttggagaga agttaacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat
     1201 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtag
//
