LOCUS       sequence-372265-       12329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12329)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD hCPS1 AD
FEATURES             Location/Qualifiers
     source          1..12329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1921..1930
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8592..8615
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(8681..9255,9256..9258,9259..9267)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8848..8865)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9075..9094
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9268..9663
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             9834..10303
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(10433..10450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10604..11192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10684..10703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11356..12147,12148..12216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11979..11998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12217..12321)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccatggcggc cctagtgttg gaggacgggt cggtcctgcg gggccagccc tttggggccg
     1981 ccgtgtcgac tgccggggaa gtggtgtttc aaaccggcat ggtcggctac cccgaggccc
     2041 tcactgatcc ctcctacaag gcacagatct tagtgctcac ctatcctctg atcggcaact
     2101 atggcatccc cccagatgaa atggatgagt tcggtctctg caagtggttt gaatcctcgg
     2161 gcatccacgt agcagcactg gtagtgggag agtgctgtcc tactcccagc cactggagtg
     2221 ccacccgcac cctgcatgag tggctgcagc agcatggcat ccctggcttg caaggagtag
     2281 acactcggga gctgaccaag aagttgcggg aacaggggtc tctgctgggg aagctggtcc
     2341 agaatggaac agaaccttca agtctgccat tcttggaccc caatgcccgc cccctggtac
     2401 cagaggtctc cattaagact ccacgggtat tcaatacagg gggtgcccct cggatccttg
     2461 ctttggactg tggcctcaag tataatcaga tccgatgcct ctgccagcgt ggggctgagg
     2521 tcactgtggt accctgggac catgcactag acagccaaga gtatgagggt ctcttcttaa
     2581 gtaatgggcc tggtgaccct gcctcctatc ccagtgtcgt atccacactg agccgtgttt
     2641 tatctgagcc taatccccga cctgtctttg ggatctgcct gggacaccag ctattggcct
     2701 tagccattgg ggccaagact tacaagatga gatatgggaa ccgaggccat aaccagccct
     2761 gcttgttggt gggctctggg cgctgctttc tgacatccca gaaccatggg tttgctgtgg
     2821 agacagactc actgccagca gactgggctc ctctcttcac caacgccaat gatggttcca
     2881 atgaaggcat tgtgcacaac agcttgcctt tcttcagtgt ccagtttcac ccagagcacc
     2941 aagctggccc ttcagatatg gaactgcttt tcgatatctt tctggaaact gtgaaagagg
     3001 ccacagctgg gaaccctggg ggccagacag ttagagagcg gctgactgag cgcctctgtc
     3061 cccctgggat tcccactccc ggctctggac ttccaccacc acgaaaggtt ctgatcctgg
     3121 gctcaggggg cctctccatt ggccaagctg gagaatttga ctactcgggc tctcaggcaa
     3181 ttaaggccct gaaggaggaa aacatccaga cgttgctgat caaccccaat attgccacag
     3241 tgcagacctc ccaggggctg gccgacaagg tctattttct tcccataaca cctcattatg
     3301 taacccaggt gatacgtaat gaacgccccg atggtgtgtt actgactttt gggggccaga
     3361 ctgctctgaa ctgtggtgtg gagctgacca aggccggggt gctggctcgg tatggggtcc
     3421 gggtcctggg cacaccagtg gagaccattg agctgaccga ggatcgacgg gcctttgctg
     3481 ccagaatggc agagatcgga gagcatgtgg ccccgagcga ggcagcaaat tctcttgaac
     3541 aggcccaggc agccgctgaa cggctggggt accctgtgct agtgcgtgca gcctttgccc
     3601 tgggtggcct gggctctggc tttgcctcta acagggagga gctctctgct ctcgtggccc
     3661 cagcttttgc ccataccagc caagtgctag tagacaagtc tctgaaggga tggaaggaga
     3721 ttgagtacga ggtggtgaga gacgcctatg gcaactgtgt cacggtgtgt aacatggaga
     3781 acttggaccc actgggcatc cacactggtg agtccatagt ggtggcccct agccagacac
     3841 tgaatgacag ggagtatcag ctcctgaggc agacagctat caaggtgacc cagcacctgg
     3901 gaattgttgg ggagtgcaat gtgcagtatg ccttgaaccc tgagtctgag cagtattaca
     3961 tcattgaagt gaatgccagg ctctctcgca gctctgccct ggccagtaag gccacaggtt
     4021 atccactggc ttatgtggca gccaagctag cattgggcat ccctttgcct gagctcagga
     4081 actctgtgac agggggtaca gcagcctttg aacccagcgt ggattattgt gtggtgaaga
     4141 ttcctcgatg ggaccttagc aagttcctgc gagtcagcac aaagattggg agctgcatga
     4201 agagcgttgg tgaagtcatg ggcattgggc gttcatttga ggaggccttc cagaaggccc
     4261 tgcgcatggt ggatgagaac tgtgtgggct ttgatcacac agtgaaacca gtcagcgata
     4321 tggagttgga gactccaaca gataagcgga tttttgtggt ggcagctgct ttgtgggctg
     4381 gttattcagt ggaccgcctg tatgagctca cacgcatcga ccgctggttc ctgcaccgaa
     4441 tgaagcgtat catcgcacat gcccagctgc tagaacaaca ccgtggacag cctttgccgc
     4501 cagacctgct gcaacaggcc aagtgtcttg gcttctcaga caaacagatt gcccttgcag
     4561 ttctgagcac agagctggct gttcgcaagc tgcgtcagga actggggatc tgtccagcag
     4621 tgaaacagat tgacacagtt gcagctgagt ggccagccca gacaaattac ctatacctaa
     4681 cgtattgggg caccacccat gacctcacct ttcgaacacc tcatgtccta gtccttggct
     4741 ctggcgtcta ccgtattggc tctagcgttg aatttgactg gtgtgctgta ggctgcatcc
     4801 agcagctccg aaagatggga tataagacca tcatggtgaa ctataaccca gagacagtca
     4861 gcaccgacta tgacatgtgt gatcgactct actttgatga gatctctttt gaggtggtga
     4921 tggacatcta tgagctcgag aaccctgaag gtgtgatcct atccatgggt ggacagctgc
     4981 ccaacaacat ggccatggcg ttgcatcggc agcagtgccg ggtgctgggc acctcccctg
     5041 aagccattga ctcggctgag aaccgtttca agttttcccg gctccttgac accattggta
     5101 tcagccagcc tcagtggagg gagctcagtg acctcgagtc tgctcgccaa ttctgccaga
     5161 ccgtggggta cccctgtgtg gtgcgcccct cctatgtgct gagcggtgct gctatgaatg
     5221 tggcctacac ggatggagac ctggagcgct tcctgagcag cgcagcagcc gtctccaaag
     5281 agcatcccgt ggtcatctcc aagttcatcc aggaggctaa ggagattgac gtggatgccg
     5341 tggcctctga tggtgtggtg gcagccatcg ccatctctga gcatgtggag aatgcaggtg
     5401 tgcattcagg tgatgcgacg ctggtgaccc ccccacaaga tatcactgcc aaaaccctgg
     5461 agcggatcaa agccattgtg catgctgtgg gccaggagct acaggtcaca ggacccttca
     5521 atctgcagct cattgccaag gatgaccagc tgaaagttat tgaatgcaac gtacgtgtct
     5581 ctcgctcctt ccccttcgtt tccaagacac tgggtgtgga cctagtagcc ttggccacgc
     5641 gggtcatcat gggggaagaa gtggaacctg tggggctaat gactggttct ggagttgtgg
     5701 gagtaaaggt gcctcagttc tccttctccc gcttggcggg tgctgacgtg gtgttgggtg
     5761 tggaaatgac cagtactggg gaggtggccg gctttgggga gagccgctgt gaggcatacc
     5821 tcaaggccat gctaagcact ggctttaaga taccccagaa aggcatcctg ataggcatcc
     5881 agcaatcatt ccggccaaga ttccttggtg tggctgaaca attacacaat gaaggtttca
     5941 agctgtttgc cacggaagcc acatcagact ggctcaacgc caacaatgtc cctgccaccc
     6001 cagtggcatg gccgtctcaa gaaggacaga atcccagcct ctcttccatc agaaaattga
     6061 ttagagatgg cagcattgac ctagtgatta accttcccaa caacaacact aaatttgtcc
     6121 atgataatta tgtgattcgg aggacagctg ttgatagtgg aatccctctc ctcactaatt
     6181 ttcaggtgac caaacttttt gctgaagctg tgcagaaatc tcgcaaggtg gactccaaga
     6241 gtcttttcca ctacaggcag tacagtgctg gaaaagcagc aatgacctcc caaaagcttg
     6301 tgcgactgcc gggattgatt gatgtccatg tgcacctgcg ggaaccaggt gggacacata
     6361 aggaggactt tgcttcaggc acagccgctg ccctggctgg gggtatcacc atggtgtgtg
     6421 ccatgcctaa tacccggccc cccatcattg acgcccctgc tctggccctg gcccagaagc
     6481 tggcagaggc tggcgcccgg tgcgactttg cgctattcct tggggcctcg tctgaaaatg
     6541 caggaacctt gggcaccgtg gccgggtctg cagccgggct gaagctttac ctcaatgaga
     6601 ccttctctga gctgcggctg gacagcgtgg tccagtggat ggagcatttc gagacatggc
     6661 cctcccacct ccccattgtg gctcacgcgg agcagcaaac cgtggctgct gtcctcatgg
     6721 tggctcagct cactcagcgc tcagtgcaca tatgtcacgt ggcacggaag gaggagatcc
     6781 tgctaattaa agctgcaaag gcacggggct tgccagtgac ctgcgaggtg gctccccacc
     6841 acctgttcct aagccatgat gacctggagc gcctggggcc tgggaagggg gaggtccggc
     6901 ctgagcttgg ctcccgccag gatgtggaag ccctgtggga gaacatggct gtcatcgact
     6961 gctttgcctc agaccatgct ccccatacct tggaggagaa gtgtgggtcc aggcccccac
     7021 ctgggttccc agggttagag accatgctgc cactactcct gacggctgta agcgagggcc
     7081 ggctcagcct ggacgacctg ctgcagcgat tgcaccacaa tcctcggcgc atctttcacc
     7141 tgcctccgca ggaggacacc tatgtggagg tggatctgga gcatgagtgg acaattccca
     7201 gccacatgcc cttctccaag gcccactgga caccttttga agggcagaaa gtgaagggca
     7261 ccgtccgccg tgtggtcctg cgaggggagg ttgcctatat cgatgggcag gttctggtac
     7321 ccccgggcta tggacaggat gtacggaagt ggccacaggg ggctgttcct cagctcccac
     7381 cctcagcccc tgccactagt gagatgacca cgacacctga aagaccccgc cgtggcatcc
     7441 cagggcttcc tgatggccgc ttccatctgc cgccccgaat ccatcgagcc tccgacccag
     7501 gtttgccagc tgaggagcca aaggagaagt cctctcggaa ggtagccgag ccagagctga
     7561 tgggaacccc tgatggcacc tgctaccctc caccaccagt accgagacag gcatctcccc
     7621 agaacctggg gacccctggc ttgctgcacc cccagacctc acccctgctg cactcattag
     7681 tgggccaaca tatcctgtcc gtccagcagt tcaccaagga tcagatgtct cacctgttca
     7741 atgtggcaca cacactgcgt atgatggtgc agaaggagcg gagcctcgac atcctgaagg
     7801 ggaaggtcat ggcctccatg ttctatgaag tgagcacacg gaccagcagc tcctttgcag
     7861 cagccatggc ccggctggga ggtgctgtgc tcagcttctc ggaagccaca tcgtccgtcc
     7921 agaagggcga atccctggct gactccgtgc agaccatgag ctgctatgcc gacgtcgtcg
     7981 tgctccggca cccccagcct ggagcagtgg agctggccgc caagcactgc cggaggccag
     8041 tgatcaatgc tggggatggg gtcggagagc accccaccca ggccctgctg gacatcttca
     8101 ccatccgtga ggagctggga actgtcaatg gcatgacgat cacgatggtg ggtgacctga
     8161 agcacggacg cacagtacat tccctggcct gcctgctcac ccagtatcgt gtcagcctgc
     8221 gctacgtggc acctcccagc ctgcgcatgc cacccactgt gcgggccttc gtggcctccc
     8281 gcggcaccaa gcaggaggaa ttcgagagca ttgaggaggc gctgcctgac actgatgtgc
     8341 tctacatgac tcgaatccag aaggaacgat ttggctctac ccaggagtac gaagcttgct
     8401 ttggtcagtt catcctcact ccccacatca tgacccgggc caagaagaag atggtggtga
     8461 tgcacccgat gccccgtgtc aacgagataa gcgtggaagt ggactcggat ccccgcgcag
     8521 cctacttccg ccaggctgag aacggcatgt acatccgcat ggctctgtta gccaccgtgc
     8581 tgggccgttt cgactacaaa gacgatgacg acaagtagga attcctgcag gcctcgaggg
     8641 ccggcgcgcc gcggccgcta cgtaaattcc gccccccccc cccctctccc tccccccccc
     8701 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     8761 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     8821 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     8881 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     8941 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     9001 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     9061 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     9121 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     9181 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     9241 aaacacgatg ataatatggc cacaaccatg gttcctttgt ctcaagaaga atccaccctc
     9301 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     9361 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     9421 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     9481 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     9541 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     9601 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     9661 taagtcgacg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     9721 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
     9781 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
     9841 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     9901 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     9961 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
    10021 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
    10081 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
    10141 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
    10201 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
    10261 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa
    10321 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa
    10381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11281 ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca atctaaagta tatatgagta
    11341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12301 taaacaaata ggggttccgc gcacatttc
//
