LOCUS       sequence-372274-        7345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Opto-T7RNAP*(563).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Baumschlager A, Aoki SK, Khammash M
  TITLE     Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for 
            Precise Spatiotemporal Gene Expression Control.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 
            10.1021/acssynbio.7b00169. Epub 2017 Oct 18.
  PUBMED    29045151
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00169"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2157-2167"
COMMENT     Sequence Label: pAB150
FEATURES             Location/Qualifiers
     source          1..7345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1807..2256
                     /codon_start=1
                     /product="""negative Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=nMagHigh1
                     /note="rapidly heterodimerizes with pMag or pMagFast2 upon 
                     blue light irradiation"
                     /translation="HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTIRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSMGFQCETE"
     terminator      2272..2358
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             2960..2980
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3021..3470
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     terminator      4460..4546
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4727..5677)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5725..5947
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6439..6533)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6554..7213)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7128..7147
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7214..7316)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
       61 ctgtttctcc atacccgttt ttttggaagg aggtaaagat ctatgaacac gattaacatc
      121 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac
      181 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg
      241 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat
      301 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac
      361 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg
      421 caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta
      481 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag
      541 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt
      601 gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc
      661 gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag
      721 gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg
      781 gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc
      841 gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg
      901 atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat
      961 tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg
     1021 cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc
     1081 gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat
     1141 tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac
     1201 atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc
     1261 aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat
     1321 aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt
     1381 tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg
     1441 gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac
     1501 tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac
     1561 gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat
     1621 tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg
     1681 agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc
     1741 tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tggcggttct
     1801 ggaggtcaca ctctttacgc ccctggagga tacgacatta tgggatattt ggatcagatt
     1861 gggaaccgcc caaaccctca ggtcgaactg gggcctgtgg acacgtcatg tgccctgatc
     1921 ctgtgcgatc tgaagcaaaa ggacactccg atcgtctacg cctcggaagc cttcttgtat
     1981 atgaccggat acagcaatgc agaggtgctc ggcaggaact gcagattcct gcagtccccc
     2041 gacgggatgg tgaaaccaaa gtcgactcgc aaatatgtgg actcgaacac gatcaacacc
     2101 atccggaagg ccatcgaccg gaacgccgag gtccaggtgg aggtggtcaa ctttaagaag
     2161 aacggccagc ggttcgtgaa ctttctgacc atcattccgg tccgggatga aaccggagag
     2221 tacagatact ccatgggatt ccagtgcgaa accgaataac ctaggaggca tcaaataaaa
     2281 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2341 ctcctgagta ggacaaatcc gccgccctag acctagctgc agctagggta cgggttttgc
     2401 tgcccgcaaa cgggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt
     2461 cagccggttt gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac
     2521 gggaggcgtc actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg
     2581 tttcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc
     2641 atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc
     2701 atctgttaca ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa
     2761 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatgcgc cgatcaacgt
     2821 ctcattttcg ccagatatcg gcgcgccgct tcgtagcatt tttatccata agattagcgg
     2881 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatgggag accacaacgg
     2941 tttccctcta gaaataattt tgtttaactt taagaaggag gtcatacccg tttttttgga
     3001 aggaggtaaa ttaattaatg cacactcttt acgcccctgg aggatacgac attatgggat
     3061 atttgcggca gattaggaac cgcccaaacc ctcaggtcga actggggcct gtggacacgt
     3121 catgtgccct gatcctgtgc gatctgaagc aaaaggacac tccgatcgtc tacgcctcgg
     3181 aagccttctt gtatatgacc ggatacagca atgcagaggt gctcggcagg aactgcagat
     3241 tcctgcagtc ccccgacggg atggtgaaac caaagtcgac tcgcaaatat gtggactcga
     3301 acacgatcaa caccatgcgg aaggccatcg accggaacgc cgaggtccag gtggaggtgg
     3361 tcaactttaa gaagaacggc cagcggttcg tgaactttct gaccatgatt ccggtccggg
     3421 atgaaaccgg agagtacaga tactccatgg gattccagtg cgaaaccgaa ggcggttctg
     3481 gaggtagtga aaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc
     3541 tacaagcaga cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca
     3601 ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg
     3661 cttacggtgt tactcgcagt gtgactaaga gttcagtcat gacgctggct tacgggtcca
     3721 aagagttcgg cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg
     3781 gcaagggtct gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt
     3841 gggaatctgt gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg
     3901 ctgctaagct gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc
     3961 gttgcgctgt gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc
     4021 ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta
     4081 acaccaacaa agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact
     4141 ttgtacacag ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt
     4201 acggaatcga atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg
     4261 cgaacctgtt caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac
     4321 tggctgattt ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc
     4381 cagcacttcc ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt
     4441 tcgcgtaatc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     4501 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac
     4561 ctagctgcag ctagggtacg ggttttgctg cccgcaaacg ggctgttctg gtgttgctag
     4621 gatccatatg gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt
     4681 tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt
     4741 atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac
     4801 cattgagatc atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct
     4861 gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat
     4921 ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt
     4981 cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc
     5041 ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg
     5101 gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca agcattaaca
     5161 tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta
     5221 gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat
     5281 gttccagatt atattttatg aattttttta actggaaaag ataaggcaat atctcttcac
     5341 taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct
     5401 caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg
     5461 ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt
     5521 tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta
     5581 gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg
     5641 ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat
     5701 tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt
     5761 gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta
     5821 gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta
     5881 taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt
     5941 ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg
     6001 ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg
     6061 gcaaatcgct gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt
     6121 cgtgacattc agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac
     6181 aggcgccttt tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg
     6241 ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt
     6301 cagcagttcc tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc
     6361 attcagactg gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt
     6421 actgtcccta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     6481 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     6541 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     6601 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     6661 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     6721 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     6781 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     6841 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     6901 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     6961 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     7021 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     7081 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     7141 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     7201 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     7261 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     7321 ctcattttcg ccagatatcg acgtc
//
