LOCUS       sequence-372285-       10091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS sgRNA expression targeting  axed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-4x(tRNA::axed{sgRNA})
FEATURES             Location/Qualifiers
     source          1..10091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        875..950
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    1658..2088
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    2122..2191
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2410..2428)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2449..2465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2449..2465)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2462..2484)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2473..2489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2497..2503,2504..2521,2522..2527))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2542..2563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2680..2697)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2851..3439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2931..2950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3610..4401,4402..4470))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4233..4252
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4471..4575)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4601..5056)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4738..4759)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4950..4969
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5183..5205
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5197..5214
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5198..5214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5221..5240
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5221..5239
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5273..5291)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5743..5814,6216..6489,6564..7218,7280..7595,7799..7930,
                     8001..8615)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9388..9421
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    9434..9864
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    9910..10004
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    join(10028..10091,1..31)
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagctc gcccggggat
       61 cgagcgcagc ggtataaaag ggcgcggggt ggctgagagc atcagttgtg aatgaatgtt
      121 cgagccgagc agacgtgccg ctgccttcgt taatatcctt tgaataagcc aactttgaat
      181 cacaagacgc ataccaaacg aattcgggct ttgagtgtgt gtagacatca agcatcggtg
      241 gttcagtggt agaatgctcg cctgccacgc gggcggcccg ggttcgattc ccggccgatg
      301 cagggggggg cgcactgggc gcgtttcaga gctatgctgg aaacagcata gcaagttgaa
      361 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgcta acaaagcacc
      421 agtggtctag tggtagaata gtaccctgcc acggtacaga cccgggttcg attcccggct
      481 ggtgcagggg gtcggcccac ggaggagttt cagagctatg ctggaaacag catagcaagt
      541 tgaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctaacaaag
      601 caccagtggt ctagtggtag aatagtaccc tgccacggta cagacccggg ttcgattccc
      661 ggctggtgca ggtgcgacgg ttctccggcg gtttcagagc tatgctggaa acagcatagc
      721 aagttgaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctaac
      781 aaagcaccag tggtctagtg gtagaatagt accctgccac ggtacagacc cgggttcgat
      841 tcccggctgg tgcagggatc accacccagg agacgtttta gagctagaaa tagcaagtta
      901 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc taacaaagca
      961 ccagtggtct agtggtagaa tagtaccctg ccacggtaca gacccgggtt cgattcccgg
     1021 ctggtgcatc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     1081 caaactacct acagagattt aaagctctaa ttaaaataac aaatcaattg ttttataata
     1141 ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt aggaagatta cgaaaaatat
     1201 aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac gccacaattg tgtttgttgc
     1261 aaataaaccc atgattattt gattaaaatt gttgttttct ttgttcatag acaatagtgt
     1321 gttttgccta aacgtgtact gcataaactc catgcgagtg tatagcgagc tagtggctaa
     1381 cgcttgcccc accaaagtag attcgtcaaa atcctcaatt tcatcaccct cctccaagtt
     1441 taacatttgg ccgtcggaat taacttctaa agatgccaca taatctaata aatgaaatag
     1501 agattcaaac gtggcgtcat cgtccgtttc gaccatttcc gaaaagaact cgggcataaa
     1561 ctctatgatt tctctggacg tggtgttgtc gaaactctca aagtacgcag tcaggaacgt
     1621 gcgcgacatg tcgtcgggaa actcgcgcgg aaacatttgg ccacgtaata agtgtgcgtt
     1681 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     1741 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     1801 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     1861 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     1921 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     1981 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     2041 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacgg atccactagt
     2101 gtcgacgatg taggtcacag tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg
     2161 ctccccgggc gcgtactcca cctcacccat ctggtccatc atgatgaacg ggtcgaggtg
     2221 gcggtagttg atcccggcga acgcgcggcg caccgggaag ccctcgccct cgaaaccgct
     2281 gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg
     2341 gggcagcgtc agcgggttct cgacggtcac ggcgggcatg tcgacactag ttctagccag
     2401 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     2461 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2521 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2581 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2641 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2701 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2761 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2821 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2881 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2941 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3001 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3061 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3121 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3181 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3241 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3301 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3361 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3421 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3481 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3541 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3601 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3661 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3721 atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc
     3781 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3841 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3901 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3961 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4021 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4081 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4141 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4201 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4261 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4321 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4381 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4441 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4501 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4561 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     4621 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4681 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     4741 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     4801 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     4861 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     4921 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     4981 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     5041 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     5101 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     5161 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     5221 taatacgact cactataggg cgaattgggt acgtaccggg cccctagtat gtatgtaagt
     5281 taataaaacc catttttgcg gaaagtagat aaaaaaaaca tttttttttt ttactgcact
     5341 ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgatg
     5401 taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt
     5461 ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct
     5521 ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc
     5581 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     5641 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg
     5701 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
     5761 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt
     5821 agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa
     5881 attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatt gcacaaacaa
     5941 atagtgacac gaaacagatt attctggtag ctgttctcgc tatataagac aatttttgag
     6001 atcatatcat gatcaagaca tctaaaggca ttcattttcg actatattct tttttacaaa
     6061 aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat
     6121 aaacctactt cgtaggatac ttcggggtac tttttgttcg gggttagatg agcataacgc
     6181 ttgtagttga tatttgagat cccctatcat tgcagggtga cagcggagcg gcttcgcaga
     6241 gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctccgg ccacccagtc
     6301 cgccggagga ctccggttca gggagcggcc aactagccga gaacctcacc tatgcctggc
     6361 acaatatgga catctttggg gcggtcaatc agccgggctc cggatggcgg cagctggtca
     6421 accggacacg cggactattc tgcaacgagc gacacatacc ggcgcccagg aaacatttgc
     6481 tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt
     6541 ccaattacca atttgaaact cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt
     6601 gatgggcagt tccggtgccg gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc
     6661 gcagggcatc caagtatcgc catccgggat gcgactgctc aatggccaac ctgtggacgc
     6721 caaggagatg caggccaggt gcgcctatgt ccagcaggat gacctcttta tcggctccct
     6781 aacggccagg gaacacctga ttttccaagc catggtgcgg atgccacgac atctgaccta
     6841 tcggcagcga gtggcccgcg tggatcaggt gatccaggag ctttcgctca gcaaatgtca
     6901 gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg tccggcggag aaaggaagcg
     6961 tctggcattc gcctccgagg ctctaaccga tccgccgctt ctgatctgcg atgagcccac
     7021 ctccggactg gactccttta ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca
     7081 gaagggcaag accgtcatcc tgaccattca tcagccgtca tccgagctgt ttgagctctt
     7141 tgacaagatc cttctgatgg ccgagggcag ggtagctttc ttgggcactc ccagcgaagc
     7201 cgtcgacttc ttttcctagt gagttcgatg tgtttattaa gggtatctag tattacataa
     7261 catctcaact cctatccagc gtgggtgccc agtgtcctac caactacaat ccggcggact
     7321 tttacgtaca ggtgttggcc gttgtgcccg gacgggagat cgagtcccgt gatcggatcg
     7381 ccaagatatg cgacaatttt gccattagca aagtagcccg ggatatggag cagttgttgg
     7441 ccaccaaaaa tctggagaag ccactggagc agccggagaa tgggtacacc tacaaggcca
     7501 cctggttcat gcagttccgg gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac
     7561 cactcctcgt aaaagtgcga cttattcaga caacggtgag tggttccagt ggaaacaaat
     7621 gatataacgc ttacaattct tggaaacaaa ttcgctagat tttagataga attgcctgat
     7681 tccacaccct tcttagtttt tttcaatgag atgtatagtt tatagttttg cagaagataa
     7741 ataaatttca tttaactcgc gaatattaat gagatgcgag taacatttta atttgcagat
     7801 ggttgccatc ttgattggcc tcatcttttt gggccaacaa ctcacgcaag tgggtgtgat
     7861 gaatatcaac ggagccatct tcctcttcct gaccaacatg acctttcaaa acgtctttgc
     7921 cacgataaat gtaagtcatg tttagaatac atttgcattt caataattta ctaactttct
     7981 aatgaatcga ttcgatttag gtgttcacct cagagctgcc agtttttatg agggaggccc
     8041 gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc gaattgccgc
     8101 tttttctcac agtgccactg gttttcacgg cgattgccta tccgatgatc ggactgcggg
     8161 ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa
     8221 cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg
     8281 gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac tcgggctcgg
     8341 tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc
     8401 tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca
     8461 ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc
     8521 tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc
     8581 tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct
     8641 tgtttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct
     8701 aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc
     8761 ccttcgaaca tccccacaag tagactttgg atttgtttct aaccaaaaga cttacacacc
     8821 tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta
     8881 tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc
     8941 gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac
     9001 gaccttggag cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg
     9061 gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc
     9121 aatatatttg tatatatttc cctcacagct atatttattc taatttaata ttatgacttt
     9181 ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg
     9241 acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt
     9301 tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg
     9361 cttggatttc actggaacta ggctagcata acttcgtata atgtatgcta tacgaagtta
     9421 tgctagcgga tcctggccac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca
     9481 tattttcggc aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt
     9541 tttagagaaa ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac
     9601 cctcttttaa taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat
     9661 aaaagattat tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
     9721 aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
     9781 atattgcata ccttttcttg ccataccatt tagccgatca attgtgctcg gcaacagtat
     9841 atttgtggtg tgccaaccaa caacagatcc aagctggccg cggctcgatc cgcttgcatg
     9901 cctgcaggtc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     9961 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggaag cttgcatgcc
    10021 tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
    10081 cgagcggagt a
//
