LOCUS       sequence-372286-        9259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS expression of GFP::NMN-Deamidase(dead).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-GFP::NMN-Deamidase{dead}
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(165..183)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(204..220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(204..220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(217..239)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    228..244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(252..258,259..276,277..282))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    297..318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(435..452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(606..1194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(686..705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1365..2156,2157..2225))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1988..2007
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2226..2330)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2356..2811)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2493..2514)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2705..2724
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2938..2960
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2952..2969
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2953..2969
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2976..2995
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2976..2994
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3028..3046)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3498..3569,3971..4244,4319..4973,5035..5350,5554..5685,
                     5756..6370)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    7145..7178
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7211..7305
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7324..7562
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7509..7530
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             join(7586..7588,7589..7591,7592..8302)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7586..7588,7589..7591,7592..8302)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7631..7652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7892..7911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8239..8260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    9136..9205
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg
       61 ggacgtgcga cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg
      121 gtcacggcgg gcatgtcgac actagttcta gccagctttt gttcccttta gtgagggtta
      181 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      241 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      301 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      361 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      421 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      481 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      541 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      601 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      661 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      721 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      781 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      841 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      901 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      961 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1021 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1081 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1141 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1201 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1261 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1321 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1381 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1441 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1501 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1561 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1621 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1681 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1741 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1801 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1861 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1921 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1981 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2041 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2101 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2161 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2221 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2281 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2341 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2401 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2461 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2521 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2581 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2641 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2701 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2761 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     2821 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2881 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2941 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
     3001 tgggtacgta ccgggcccct agtatgtatg taagttaata aaacccattt ttgcggaaag
     3061 tagataaaaa aaacattttt tttttttact gcactggata tcattgaact tatctgatca
     3121 gttttaaatt tacttcgatc caagggtatt tgatgtacca ggttctttcg attacctctc
     3181 actcaaaatg acattccact caaagtcagc gctgtttgcc tccttctctg tccacagaaa
     3241 tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc gccaccgttt gtagcgttac
     3301 gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg aagctccaag
     3361 cggtttacgc catcaattaa acacaaagtg ctgtgccaaa actcctctcg cttcttattt
     3421 ttgtttgttt tttgagtgat tggggtggtg attggttttg ggtgggtaag caggggaaag
     3481 tgtgaaaaat cccggcaatg ggccaagagg atcaggagct attaattcgc ggaggcagca
     3541 aacacccatc tgccgagcat ctgaacaatg tgagtagtac atgtgcatac atcttaagtt
     3601 cacttgatct ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga
     3661 cccacaaaaa tcccaaaccg caattgcaca aacaaatagt gacacgaaac agattattct
     3721 ggtagctgtt ctcgctatat aagacaattt ttgagatcat atcatgatca agacatctaa
     3781 aggcattcat tttcgactat attctttttt acaaaaaata taacaaccag atattttaag
     3841 ctgatcctag atgcacaaaa aataaataaa agtataaacc tacttcgtag gatacttcgg
     3901 ggtacttttt gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct
     3961 atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca
     4021 ggccaaaaac tacggcacgc tccggccacc cagtccgccg gaggactccg gttcagggag
     4081 cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt
     4141 caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa
     4201 cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc
     4261 agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt
     4321 tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag
     4381 acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc
     4441 gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc
     4501 tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc
     4561 caagccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat
     4621 caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc
     4681 agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggctcta
     4741 accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc ctttaccgcc
     4801 cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc
     4861 attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag
     4921 ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt
     4981 cgatgtgttt attaagggta tctagtatta cataacatct caactcctat ccagcgtggg
     5041 tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt
     5101 gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgccat
     5161 tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatctgg agaagccact
     5221 ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt
     5281 cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat
     5341 tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa
     5401 acaaattcgc tagattttag atagaattgc ctgattccac acccttctta gtttttttca
     5461 atgagatgta tagtttatag ttttgcagaa gataaataaa tttcatttaa ctcgcgaata
     5521 ttaatgagat gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc
     5581 tttttgggcc aacaactcac gcaagtgggt gtgatgaata tcaacggagc catcttcctc
     5641 ttcctgacca acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcatgtttag
     5701 aatacatttg catttcaata atttactaac tttctaatga atcgattcga tttaggtgtt
     5761 cacctcagag ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac
     5821 atactttctg ggcaaaacga ttgccgaatt gccgcttttt ctcacagtgc cactggtctt
     5881 cacggcgatt gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg
     5941 cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg
     6001 cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct
     6061 gctctttggc ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc
     6121 gtacctctca tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt
     6181 ggagccgggc gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt
     6241 catcctggag acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc
     6301 cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg
     6361 caaggagtag ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac
     6421 catcgtgttt actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata
     6481 aaaacaagat atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga
     6541 ctttggattt gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc
     6601 gtttatcgct acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc
     6661 gccctcttca taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc
     6721 cacccgctct ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag
     6781 ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct caatagaaat
     6841 tagtaataaa tatttgtatg tacaatttat ttgctccaat atatttgtat atatttccct
     6901 cacagctata tttattctaa tttaatatta tgacttttta aggtaatttt ttgtgacctg
     6961 ttcggagtga ttagcgttac aatttgaact gaaagtgaca tccagtgttt gttccttgtg
     7021 tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt ttatatatat caaaaataac
     7081 aactataata ataagaatac atttaattta gaaaatgctt ggatttcact ggaactaggc
     7141 tagcataact tcgtataatg tatgctatac gaagttatgc tagcggatcc aagcttgcat
     7201 gcctgcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
     7261 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag actctagcga
     7321 gcgccggagt ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca
     7381 aagtgaacac gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa
     7441 acaatctgca gtaaagtgca agttaaagtg aatcaattaa aagtaaccag caaccaagta
     7501 aatcaactgc aactactgaa atctgccaag aagtaattat tgaatacaag aagagaactc
     7561 tgaataggga attgggaatt ccaacatggt gagcaagggc gaggagctgt tcaccggggt
     7621 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     7681 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     7741 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     7801 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     7861 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     7921 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     7981 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     8041 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     8101 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     8161 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     8221 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     8281 cggcatggac gagctgtaca agtccggact cagatctact gacagtgaac tgatgcagtt
     8341 aagtgaacag gttgggcagg cgctgaaagc ccgtggcgca accgtaacaa ctgccgagtc
     8401 ttgtaccggt ggttgggtag cgaaagtgat taccgatatt gccggtagct ccgcctggtt
     8461 tgaacgcgga tttgtcacct acagtaacga agcccaagcg cagatgatcg gcgtacgcga
     8521 agagacgctg gcgcagcatg gcgcggtgag tgaacccgtc gtggtggaaa tggcgatagg
     8581 cgcactgaaa gcggctcgtg ctgattatgc cgtgtctatt agtggtatcg ccgggccgga
     8641 tggcggcagt gaagagaagc ctgtcggcac cgtctggttt gcttttgcca ctgcccgcgg
     8701 tgaaggcatt acccggcggg aatgcttcag cggcgaccgt gatgcggtgc gtcgtcaggc
     8761 tactgcgtat gcattgcaga ccttgtggca acaatttcta caaaacactt gagacgtcta
     8821 tcgataccgt cgactaaagc caaatagaaa attattcagt tcctggctta agtttttaaa
     8881 agtgatatta tttatttggt tgtaaccaac caaaagaatg taaataacta atacataatt
     8941 atgttagttt taagttagca acaaattgat tttagctata ttagctactt ggttaataaa
     9001 tagaatatat ttatttaaag ataattgcgt ttttattgtc agggagtgag tttgcttaaa
     9061 aactcgttta gatccgcggc cgcggctcga gggtacctct agagatccac tagtgtcgac
     9121 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
     9181 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
     9241 gttgatcccg gcgaacgcg
//
