LOCUS       sequence-372287-       11861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS expression of dSarm(isoE,K450R)::FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-dsarm(E,K450R)::flag
FEATURES             Location/Qualifiers
     source          1..11861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(112..133)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     324..343
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     557..579
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     571..588
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     572..588
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     595..614
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        595..613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(647..665)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1117..1188,1590..1863,1938..2592,2654..2969,3173..3304,
                     3375..3989)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4764..4797
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4941..5179
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5126..5147
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             5593..5613
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             8355..8378
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          8669..8734
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8864..8884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9156..9290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9193..9212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9247..9266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9357..9426
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9645..9663)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9684..9700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9684..9700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9697..9719)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9708..9724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9732..9738,9739..9756,9757..9762))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9777..9798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9915..9932)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10086..10674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10166..10185)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10845..11636,11637..11705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11468..11487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11706..11810)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(11836..11861,1..430))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
       61 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
      121 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
      181 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
      241 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
      301 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
      361 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
      421 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      481 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      541 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
      601 gactcactat agggcgaatt gggtacgtac cgggccccta gtatgtatgt aagttaataa
      661 aacccatttt tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat
      721 cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag
      781 gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct
      841 ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg
      901 ccaccgtttg tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt
      961 ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa
     1021 ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg
     1081 gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta
     1141 ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca
     1201 tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc
     1261 tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg
     1321 acacgaaaca gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata
     1381 tcatgatcaa gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat
     1441 aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct
     1501 acttcgtagg atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag
     1561 ttgatatttg agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca
     1621 ttaaccaggg cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg
     1681 aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata
     1741 tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga
     1801 cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga
     1861 acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt
     1921 accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg
     1981 cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg
     2041 catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga
     2101 gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc
     2161 cagggaacac ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca
     2221 gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac
     2281 gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc
     2341 attcgcctcc gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg
     2401 actggactcc tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg
     2461 caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa
     2521 gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga
     2581 cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc
     2641 aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg
     2701 tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga
     2761 tatgcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca
     2821 aaaatctgga gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt
     2881 tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc
     2941 tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata
     3001 acgcttacaa ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca
     3061 cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat
     3121 ttcatttaac tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc
     3181 catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat
     3241 caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat
     3301 aaatgtaagt catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa
     3361 tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc
     3421 gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc
     3481 tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag
     3541 tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct
     3601 tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc
     3661 cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag
     3721 tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga
     3781 ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt
     3841 gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc
     3901 tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc
     3961 taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt
     4021 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     4081 aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc
     4141 gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca
     4201 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     4261 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     4321 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     4381 ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc
     4441 atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata
     4501 tatttgtata tatttccctc acagctatat ttattctaat ttaatattat gactttttaa
     4561 ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat
     4621 ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt
     4681 tatatatatc aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg
     4741 gatttcactg gaactaggct agcataactt cgtataatgt atgctatacg aagttatgct
     4801 agcggatcca aaagctttcc ggatccaagc ttgcatgcct gcaggtcgga gtactgtcct
     4861 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     4921 agcggagact ctagcgagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     4981 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     5041 gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag
     5101 taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga
     5161 atacaagaag agaactctga atagggaatt gggaattcat gtccaatcag gcaccctggc
     5221 ccgttcgcaa gggcatcttc cgctcgagcg gtcagtcgga tttcacgcca acccgatcgc
     5281 ctagtcccat tgtggagatg ccattatccc cgccgccggt ggccacgccc tcgaatagat
     5341 ttggaatcaa ttcaccgctc tccccaccgc cgcagcctat tcaagtggtg gcgggttcaa
     5401 cgacagccac taccatgtcc actgcgtccg ctgccagagt gtctggtgca gctgcctcca
     5461 cttcatcctc gtcctcctct tcttcgtcgt cgcagtgctc gtcgcaaacc tcgtcgagtt
     5521 ctagttcgca caccagagta cgtaaaagtt ctaacccgcc cccacagcca attaccagtt
     5581 gtccactgtc gccaccacca ccaccaccac cacagcaaca acaacaacta ccacaacaac
     5641 tgccacctcc aacgcccatc aacaacacca accactcagc aattacaaca cccaatcaca
     5701 acaataacca caactgcaat caaatgagaa atgtacgcga aattcccatt gaggtggagc
     5761 agagcaagga agccctctca ctccgcaccg gcgacatcac acagcaggcg agcaacaatg
     5821 tggcggcctc cagtattacg gtgcaaagtg agaatttctc cgcggacaag aaggcgatat
     5881 cccagtcgca gcaatcgcag acgatgacct ccaacgggat catcagtcag gagaaacatg
     5941 tatcctccgc ctcgcaggcc aactactcga tgtcgcacaa gggcgtttcc agtaccggca
     6001 gtagcatgat caccagctcc tcgcaaatgt ccgcgatgaa tggccagatg ctgaagctcg
     6061 ccgatctcaa gttggatgac ctcaagtcgc tgacagctgg cagtgggcag caggagatcg
     6121 agcagaccat caacaagtat tccaatatgc tgacctcaat agtgagctcc ctgcaagaag
     6181 atgagcgagg tggtagtgcc ataaccgtcc acgatgttgg tggcaagaag tctcagtacc
     6241 tggagaagat caacgaagtc attcgtcgag cttgggcagt gcccacacat ggccacgagc
     6301 tgggctactc attgtgcaat tccctgcgcc aaagtggcgg cctcgatctt ctcatgaaga
     6361 actgcgttaa gcccgatctc cagttttcct ccgcacagct tcttgagcag tgcctaacca
     6421 ccgagaaccg caaacatgtg gtggataatg gtctggataa ggtggtcaat gtggcctgtg
     6481 tctgcacaaa gaattccaat atggagcact cccgcgtggg cactggcatc ctggagcatc
     6541 tgttcagaca ctccgagggc acctgttcgg atgtgataag gctaggtggt ctggatgccg
     6601 tgctctttga gtgccgcacc agtgatttgg aaactctgcg acactgcgct agtgcactgg
     6661 caaatttatc tctatatggt ggagccgaga atcaggagga gatgatccta cgcaaggtgc
     6721 ccatgtggtt gttcccattg gccttccaca acgatgataa catcaagtac tatgcctgct
     6781 tggccattgc agtgctggta gccaacaagg agattgaagc cgaggtgctg aagtctggat
     6841 gcttggattt ggtggaaccc tttgtcacct cccacgaccc ctcggctttt gcgagatcca
     6901 atctggcaca tgcccacggg caaagcaaac attggctgaa aagattggtt ccagttttga
     6961 gttccaatcg cgaagaggcc cgcaatctgg ctgccttcca tttttgcatg gaggcgggca
     7021 tcaagaggga gcagggtaac accgacatct tccgcgagat caacgctatt gaagctttga
     7081 aaaatgtggc cagctgtcca aatgccattg catccaagtt cgccgctcag gctctgagat
     7141 tgattggaga gacagttccg cacaagctgt cccaacaggt tcccttgtgg tccgtggagg
     7201 atgtgcagga gtgggtaaag caaatcggtt tcaatgacta catcgacaag ttcaacgagt
     7261 cccaggtgga cggggatctt ttactgaagc tcaatcagga taacctccgg gctgatattg
     7321 gaatcggtaa tggaatactt ctaaagcgtt ttgaacgcga gctgcaaaat ctcaagcgta
     7381 tggctgacta ctcatccaag gacacggcca agatgcacca atttctctcg gagatcggta
     7441 ctgattactg cacctacacg tatgccatgc tgaatgctgg aattgacaag tgtgcactgc
     7501 cgcatgtcaa tgaggatatg ctgatgacag agtgtggcat ccacaactcg atccatcgtc
     7561 tgagaattct caatgcggtc aaaaacttgg agaattcgct gcccagctcg tcggaggaga
     7621 acatggccaa gacgctagat gttttcgtta gctataggcg atcaaacggc tcccaactag
     7681 ccagtcttct caaggtgcat ctgcagcttc gtggtttctc cgttttcatc gacgtggagc
     7741 gcctggaggc gggcaaattt gacaacggcc tattgaacag tattcgacag gcaaagaact
     7801 ttgtcttagt attaactccc gatgccctgc accgctgcat taacgacgag gactgcaagg
     7861 actgggtgca tcgcgaaatc gtggctgccc tgaactcgaa ctgcaacatt atacccatca
     7921 tagaccagca attcgattgg cccgaggtgg agcgacttcc cgaggacatg cgcagtgtgg
     7981 cccattttaa cggtgttaat tggatccacg actaccagga cgcatgcatc gataagctcg
     8041 aaagattttt gcgcggcgaa aagaatatcg atcgcattgc ggcgatggtg cccggcaccc
     8101 ccggttcggt ctcataccaa agaatgcaca gcaacgactc cgactaccag agcggaggag
     8161 caggcgccgg atcaggagca ggaactggag gaggaggtgg tggcggcgtt acgggcagtg
     8221 tggtggatgg cctgatggtg gcggccaatg gcagcggaca aggtgggcca acgagtacta
     8281 catctactac ttcttctact cccaactcaa attcaagctc aagctctaac cagagtcccc
     8341 cagcggctcc agcggactac aaagacgatg acgacaagta ggaattccgg cgatgctgat
     8401 ccgagcagtc cgatggggag gaggaggagg aggaggagga cggcgatgag gaggaggaag
     8461 atgatggatc cgatgaggaa aatggaaatg gaaatagagg gggcagaaat cgacccagag
     8521 acaacggagg atgagatgat gatcccacat agacttgttg cattaaacga atattagcgc
     8581 ttatgtctag aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     8641 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     8701 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     8761 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     8821 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     8881 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     8941 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     9001 atacaagaaa attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca
     9061 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     9121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     9181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9241 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ggatccgcgg
     9301 ccgcggctcg agggtacctc tagagatcca ctagtgtcga cgatgtaggt cacggtctcg
     9361 aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca
     9421 cccatctggt ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg
     9481 cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg
     9541 ggacgtgcga cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg
     9601 gtcacggcgg gcatgtcgac actagttcta gccagctttt gttcccttta gtgagggtta
     9661 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9721 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9781 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9841 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9901 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9961 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10021 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10081 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10141 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10201 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10261 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10321 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10381 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10441 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10501 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10561 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10621 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10681 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10741 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10801 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10861 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10921 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10981 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11041 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11101 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11161 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11221 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11281 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11341 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11401 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11461 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11521 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11581 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11641 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11701 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11761 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11821 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt a
//
