LOCUS       sequence-372288-       11856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS expression of dSarm(isoE,K450R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 11856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-dsarm(E,K450R)
FEATURES             Location/Qualifiers
     source          1..11856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          285..350
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             480..500
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    772..906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(809..828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     863..882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    973..1042
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(1261..1279)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1300..1316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1300..1316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1313..1335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1324..1340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1348..1354,1355..1372,1373..1378))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1393..1414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1531..1548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1702..2290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1782..1801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2461..3252,3253..3321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3084..3103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3322..3426)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3452..3907)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3589..3610)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3801..3820
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4034..4056
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4048..4065
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4049..4065
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4072..4091
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4072..4090
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4124..4142)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4594..4665,5067..5340,5415..6069,6131..6446,6650..6781,
                     6852..7466)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    8241..8274
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8324..8418
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        8437..8675
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     8622..8643
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             9089..9109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 ttccggcgat gctgatccga gcagtccgat ggggaggagg aggaggagga ggaggacggc
       61 gatgaggagg aggaagatga tggatccgat gaggaaaatg gaaatggaaa tagagggggc
      121 agaaatcgac ccagagacaa cggaggatga gatgatgatc ccacatagac ttgttgcatt
      181 aaacgaatat tagcgcttat gtctagagga tctttgtgaa ggaaccttac ttctgtggtg
      241 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
      301 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
      361 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
      421 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
      481 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
      541 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
      601 aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga
      661 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      721 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
      781 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
      841 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
      901 tggatcggat ccgcggccgc ggctcgaggg tacctctaga gatccactag tgtcgacgat
      961 gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg
     1021 cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt
     1081 gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt
     1141 ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt
     1201 cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca gcttttgttc
     1261 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1321 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1381 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1441 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1501 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1561 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1621 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1681 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1741 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1801 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1861 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1921 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1981 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2041 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2101 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2161 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2221 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2281 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2341 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2401 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2461 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2521 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2581 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2641 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2701 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2761 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2821 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2881 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2941 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3001 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3061 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3121 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3181 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3241 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3301 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3361 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3421 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     3481 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3541 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     3601 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3661 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3721 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3781 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3841 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3901 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     3961 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     4021 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     4081 tcactatagg gcgaattggg tacgtaccgg gcccctagta tgtatgtaag ttaataaaac
     4141 ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat
     4201 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt
     4261 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
     4321 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
     4381 ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc
     4441 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
     4501 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
     4561 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
     4621 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
     4681 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
     4741 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca
     4801 cgaaacagat tattctggta gctgttctcg ctatataaga caatttttga gatcatatca
     4861 tgatcaagac atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac
     4921 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
     4981 tcgtaggata cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg
     5041 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     5101 accagggctt cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg
     5161 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     5221 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     5281 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     5341 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     5401 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     5461 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     5521 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     5581 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     5641 ggaacacctg attttccaag ccatggtgcg gatgccacga catctgacct atcggcagcg
     5701 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     5761 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     5821 cgcctccgag gctctaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     5881 ggactccttt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     5941 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     6001 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     6061 cttttcctag tgagttcgat gtgtttatta agggtatcta gtattacata acatctcaac
     6121 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     6181 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     6241 gcgacaattt tgccattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
     6301 atctggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
     6361 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
     6421 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
     6481 cttacaattc ttggaaacaa attcgctaga ttttagatag aattgcctga ttccacaccc
     6541 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaagata aataaatttc
     6601 atttaactcg cgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat
     6661 cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa
     6721 cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa
     6781 tgtaagtcat gtttagaata catttgcatt tcaataattt actaactttc taatgaatcg
     6841 attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac
     6901 tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattgccg ctttttctca
     6961 cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc
     7021 tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg
     7081 gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg
     7141 ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat
     7201 acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta
     7261 accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc
     7321 ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg
     7381 actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa
     7441 gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt
     7501 tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat
     7561 tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa
     7621 catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac
     7681 cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat
     7741 acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg
     7801 ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg
     7861 gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt
     7921 tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat
     7981 ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt
     8041 aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca
     8101 gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat
     8161 atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa atgcttggat
     8221 ttcactggaa ctaggctagc ataacttcgt ataatgtatg ctatacgaag ttatgctagc
     8281 ggatccaaaa gctttccgga tccaagcttg catgcctgca ggtcggagta ctgtcctccg
     8341 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
     8401 ggagtactgt cctccgagcg gagactctag cgagcgccgg agtataaata gaggcgcttc
     8461 gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct
     8521 aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa
     8581 gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc
     8641 aagaagtaat tattgaatac aagaagagaa ctctgaatag ggaattggga attcatgtcc
     8701 aatcaggcac cctggcccgt tcgcaagggc atcttccgct cgagcggtca gtcggatttc
     8761 acgccaaccc gatcgcctag tcccattgtg gagatgccat tatccccgcc gccggtggcc
     8821 acgccctcga atagatttgg aatcaattca ccgctctccc caccgccgca gcctattcaa
     8881 gtggtggcgg gttcaacgac agccactacc atgtccactg cgtccgctgc cagagtgtct
     8941 ggtgcagctg cctccacttc atcctcgtcc tcctcttctt cgtcgtcgca gtgctcgtcg
     9001 caaacctcgt cgagttctag ttcgcacacc agagtacgta aaagttctaa cccgccccca
     9061 cagccaatta ccagttgtcc actgtcgcca ccaccaccac caccaccaca gcaacaacaa
     9121 caactaccac aacaactgcc acctccaacg cccatcaaca acaccaacca ctcagcaatt
     9181 acaacaccca atcacaacaa taaccacaac tgcaatcaaa tgagaaatgt acgcgaaatt
     9241 cccattgagg tggagcagag caaggaagcc ctctcactcc gcaccggcga catcacacag
     9301 caggcgagca acaatgtggc ggcctccagt attacggtgc aaagtgagaa tttctccgcg
     9361 gacaagaagg cgatatccca gtcgcagcaa tcgcagacga tgacctccaa cgggatcatc
     9421 agtcaggaga aacatgtatc ctccgcctcg caggccaact actcgatgtc gcacaagggc
     9481 gtttccagta ccggcagtag catgatcacc agctcctcgc aaatgtccgc gatgaatggc
     9541 cagatgctga agctcgccga tctcaagttg gatgacctca agtcgctgac agctggcagt
     9601 gggcagcagg agatcgagca gaccatcaac aagtattcca atatgctgac ctcaatagtg
     9661 agctccctgc aagaagatga gcgaggtggt agtgccataa ccgtccacga tgttggtggc
     9721 aagaagtctc agtacctgga gaagatcaac gaagtcattc gtcgagcttg ggcagtgccc
     9781 acacatggcc acgagctggg ctactcattg tgcaattccc tgcgccaaag tggcggcctc
     9841 gatcttctca tgaagaactg cgttaagccc gatctccagt tttcctccgc acagcttctt
     9901 gagcagtgcc taaccaccga gaaccgcaaa catgtggtgg ataatggtct ggataaggtg
     9961 gtcaatgtgg cctgtgtctg cacaaagaat tccaatatgg agcactcccg cgtgggcact
    10021 ggcatcctgg agcatctgtt cagacactcc gagggcacct gttcggatgt gataaggcta
    10081 ggtggtctgg atgccgtgct ctttgagtgc cgcaccagtg atttggaaac tctgcgacac
    10141 tgcgctagtg cactggcaaa tttatctcta tatggtggag ccgagaatca ggaggagatg
    10201 atcctacgca aggtgcccat gtggttgttc ccattggcct tccacaacga tgataacatc
    10261 aagtactatg cctgcttggc cattgcagtg ctggtagcca acaaggagat tgaagccgag
    10321 gtgctgaagt ctggatgctt ggatttggtg gaaccctttg tcacctccca cgacccctcg
    10381 gcttttgcga gatccaatct ggcacatgcc cacgggcaaa gcaaacattg gctgaaaaga
    10441 ttggttccag ttttgagttc caatcgcgaa gaggcccgca atctggctgc cttccatttt
    10501 tgcatggagg cgggcatcaa gagggagcag ggtaacaccg acatcttccg cgagatcaac
    10561 gctattgaag ctttgaaaaa tgtggccagc tgtccaaatg ccattgcatc caagttcgcc
    10621 gctcaggctc tgagattgat tggagagaca gttccgcaca agctgtccca acaggttccc
    10681 ttgtggtccg tggaggatgt gcaggagtgg gtaaagcaaa tcggtttcaa tgactacatc
    10741 gacaagttca acgagtccca ggtggacggg gatcttttac tgaagctcaa tcaggataac
    10801 ctccgggctg atattggaat cggtaatgga atacttctaa agcgttttga acgcgagctg
    10861 caaaatctca agcgtatggc tgactactca tccaaggaca cggccaagat gcaccaattt
    10921 ctctcggaga tcggtactga ttactgcacc tacacgtatg ccatgctgaa tgctggaatt
    10981 gacaagtgtg cactgccgca tgtcaatgag gatatgctga tgacagagtg tggcatccac
    11041 aactcgatcc atcgtctgag aattctcaat gcggtcaaaa acttggagaa ttcgctgccc
    11101 agctcgtcgg aggagaacat ggccaagacg ctagatgttt tcgttagcta taggcgatca
    11161 aacggctccc aactagccag tcttctcaag gtgcatctgc agcttcgtgg tttctccgtt
    11221 ttcatcgacg tggagcgcct ggaggcgggc aaatttgaca acggcctatt gaacagtatt
    11281 cgacaggcaa agaactttgt cttagtatta actcccgatg ccctgcaccg ctgcattaac
    11341 gacgaggact gcaaggactg ggtgcatcgc gaaatcgtgg ctgccctgaa ctcgaactgc
    11401 aacattatac ccatcataga ccagcaattc gattggcccg aggtggagcg acttcccgag
    11461 gacatgcgca gtgtggccca ttttaacggt gttaattgga tccacgacta ccaggacgca
    11521 tgcatcgata agctcgaaag atttttgcgc ggcgaaaaga atatcgatcg cattgcggcg
    11581 atggtgcccg gcacccccgg ttcggtctca taccaaagaa tgcacagcaa cgactccgac
    11641 taccagagcg gaggagcagg cgccggatca ggagcaggaa ctggaggagg aggtggtggc
    11701 ggcgttacgg gcagtgtggt ggatggcctg atggtggcgg ccaatggcag cggacaaggt
    11761 gggccaacga gtactacatc tactacttct tctactccca actcaaattc aagctcaagc
    11821 tctaaccaga gtcccccagc ggctccagcg taggaa
//
