LOCUS       sequence-372293-        7345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Opto-T7RNAP*(179).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Baumschlager A, Aoki SK, Khammash M
  TITLE     Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for 
            Precise Spatiotemporal Gene Expression Control.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 
            10.1021/acssynbio.7b00169. Epub 2017 Oct 18.
  PUBMED    29045151
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00169"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2157-2167"
COMMENT     Sequence Label: pAB174
FEATURES             Location/Qualifiers
     source          1..7345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             661..1110
                     /codon_start=1
                     /product="""negative Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=nMagHigh1
                     /note="rapidly heterodimerizes with pMag or pMagFast2 upon 
                     blue light irradiation"
                     /translation="HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTIRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTIIPVRDETGEYRYSMGFQCETE"
     terminator      1126..1212
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1814..1834
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1875..2324
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     terminator      4466..4552
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4733..5683)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5731..5953
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6445..6539)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6560..7219)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7134..7153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7220..7322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtctagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
       61 tctctactgt ttctccatac ccgttttttt ggaaggaggt aaagatctat gaacacgatt
      121 aacatcgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg
      181 gctgaccatt acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac
      241 gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt
      301 gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc
      361 atcaacgact ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag
      421 ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct
      481 tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc
      541 attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa
      601 aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagggcgg ttctggaggt
      661 cacactcttt acgcccctgg aggatacgac attatgggat atttggatca gattgggaac
      721 cgcccaaacc ctcaggtcga actggggcct gtggacacgt catgtgccct gatcctgtgc
      781 gatctgaagc aaaaggacac tccgatcgtc tacgcctcgg aagccttctt gtatatgacc
      841 ggatacagca atgcagaggt gctcggcagg aactgcagat tcctgcagtc ccccgacggg
      901 atggtgaaac caaagtcgac tcgcaaatat gtggactcga acacgatcaa caccatccgg
      961 aaggccatcg accggaacgc cgaggtccag gtggaggtgg tcaactttaa gaagaacggc
     1021 cagcggttcg tgaactttct gaccatcatt ccggtccggg atgaaaccgg agagtacaga
     1081 tactccatgg gattccagtg cgaaaccgaa taacctagga ggcatcaaat aaaacgaaag
     1141 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     1201 agtaggacaa atccgccgcc ctagacctag ctgcagctag ggtacgggtt ttgctgcccg
     1261 caaacgggct gttctggtgt tgctagtttg ttatcagaat cgcagatccg gcttcagccg
     1321 gtttgccggc tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg
     1381 cgtcactggc tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag
     1441 gctgtctatg tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc
     1501 tagttgcttt gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt
     1561 tacattgtcg atctgttcat ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc
     1621 tgatgtatct atctttttta caccgttttc atctgtgcat gcgccgatca acgtctcatt
     1681 ttcgccagat atcggcgcgc cgcttcgtag catttttatc cataagatta gcggatccta
     1741 cctgacgctt tttatcgcaa ctctctactg tttctccatg ggagaccaca acggtttccc
     1801 tctagaaata attttgttta actttaagaa ggaggtcata cccgtttttt tggaaggagg
     1861 taaattaatt aatgcacact ctttacgccc ctggaggata cgacattatg ggatatttgc
     1921 ggcagattag gaaccgccca aaccctcagg tcgaactggg gcctgtggac acgtcatgtg
     1981 ccctgatcct gtgcgatctg aagcaaaagg acactccgat cgtctacgcc tcggaagcct
     2041 tcttgtatat gaccggatac agcaatgcag aggtgctcgg caggaactgc agattcctgc
     2101 agtcccccga cgggatggtg aaaccaaagt cgactcgcaa atatgtggac tcgaacacga
     2161 tcaacaccat gcggaaggcc atcgaccgga acgccgaggt ccaggtggag gtggtcaact
     2221 ttaagaagaa cggccagcgg ttcgtgaact ttctgaccat gattccggtc cgggatgaaa
     2281 ccggagagta cagatactcc atgggattcc agtgcgaaac cgaaggcggt tctggaggta
     2341 aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg
     2401 cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc
     2461 tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag
     2521 actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg
     2581 cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg
     2641 gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc
     2701 acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga
     2761 ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg
     2821 taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac
     2881 tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg
     2941 ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt
     3001 tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca
     3061 tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga
     3121 ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc
     3181 tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct gagcgcatca
     3241 agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca
     3301 cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg
     3361 gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt
     3421 gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta
     3481 acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg
     3541 agattctaca agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg
     3601 agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat
     3661 ggctggctta cggtgttact cgcagtgtga ctaagagttc agtcatgacg ctggcttacg
     3721 ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg
     3781 attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc
     3841 tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta
     3901 agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc
     3961 gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca
     4021 agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc ttacagccta
     4081 ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc
     4141 ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg
     4201 agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc attccggctg
     4261 acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg
     4321 atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca
     4381 aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact
     4441 tcgcgttcgc gtaatctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     4501 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     4561 ctagacctag ctgcagctag ggtacgggtt ttgctgcccg caaacgggct gttctggtgt
     4621 tgctaggatc catatggaca gttttccctt tgatatgtaa cggtgaacag ttgttctact
     4681 tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc
     4741 ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga
     4801 acgaaccatt gagatcatac ttactttgca tgtcactcaa aaattttgcc tcaaaactgg
     4861 tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt
     4921 aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct
     4981 caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag
     5041 tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac
     5101 ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca
     5161 ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt
     5221 gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact
     5281 taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct
     5341 cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga
     5401 aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc
     5461 tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt
     5521 attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt
     5581 tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac
     5641 taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta
     5701 ggtgatttta atcactatac caattgagat gggctagtca atgataatta ctagtccttt
     5761 tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt
     5821 ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag
     5881 tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc
     5941 ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc
     6001 tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa
     6061 gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt
     6121 ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg
     6181 cactacaggc gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc
     6241 gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt
     6301 gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga
     6361 caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc
     6421 cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     6481 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     6541 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     6601 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     6661 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     6721 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     6781 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     6841 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     6901 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     6961 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     7021 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     7081 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     7141 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     7201 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     7261 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     7321 caacgtctca ttttcgccag atatc
//
