LOCUS       sequence-372294-        9708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/UAS sgRNA expression targeting  hiw.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Llobet Rosell A, Paglione M, Gilley J, Kocia M, Perillo G, 
            Gasparrini M, Cialabrini L, Raffaelli N, Angeletti C, Orsomando G, 
            Wu PH, Coleman MP, Loreto A, Neukomm LJ
  TITLE     The NAD(+) precursor NMN activates dSarm to trigger axon 
            degeneration in Drosophila.
  JOURNAL   Elife. 2022 Dec 7;11:e80245. doi: 10.7554/eLife.80245.
  PUBMED    36476387
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-12-7"; volume: "11:e80245. doi"; pages: " 
            10.7554/eLife.80245"
COMMENT     Sequence Label: UAS-4x(tRNA::hiw{sgRNA})
FEATURES             Location/Qualifiers
     source          1..9708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2762..2792,2793..2994)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3190..3428
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3375..3396
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     misc_RNA        4117..4192
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     intron          4354..4419
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4549..4569
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4841..4975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4878..4897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4932..4951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5002..5020)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5472..5543,5945..6218,6293..6947,7009..7324,7528..7659,
                     7730..8344)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9123..9677,9678..9708)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     2701 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     2761 ccatgatgaa ataacataag gtggtcccgt cggcaagaga catccactta acgtatgctt
     2821 gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct gagtgagaca
     2881 gcgatatgat tgttgattaa cccttagcat gtccgtgggg tttgaattaa ctcataatat
     2941 taattagacg aaattatttt taaagtttta tttttaataa tttgcgagta cgcaaagctt
     3001 ggctgcatcc aacgcgttgg gagctctccg gatccaagct tggatccggg taatcgctta
     3061 tcctcggata aacaattatc ctcacgggta atcgcttatc cgctcgggta atcgcttatc
     3121 ctcgggtaat cgcttatcct taagcttgat atcgaattgc tgcagcccga gcggagactc
     3181 tagcgagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
     3241 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
     3301 agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
     3361 caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
     3421 gaactctgaa tagggaattg ggaattcggg ctttgagtgt gtgtagacat caagcatcgg
     3481 tggttcagtg gtagaatgct cgcctgccac gcgggcggcc cgggttcgat tcccggccga
     3541 tgcatgcaag gtggccagca ccacgtttca gagctatgct ggaaacagca tagcaagttg
     3601 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc taacaaagca
     3661 ccagtggtct agtggtagaa tagtaccctg ccacggtaca gacccgggtt cgattcccgg
     3721 ctggtgcagg atgactattt ggagcagggt ttcagagcta tgctggaaac agcatagcaa
     3781 gttgaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctaacaa
     3841 agcaccagtg gtctagtggt agaatagtac cctgccacgg tacagacccg ggttcgattc
     3901 ccggctggtg cagggggtgg tgcggtggcg gggtttcaga gctatgctgg aaacagcata
     3961 gcaagttgaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgcta
     4021 acaaagcacc agtggtctag tggtagaata gtaccctgcc acggtacaga cccgggttcg
     4081 attcccggct ggtgcaggaa gcggcaaggg cgggcggttt tagagctaga aatagcaagt
     4141 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctaacaaag
     4201 caccagtggt ctagtggtag aatagtaccc tgccacggta cagacccggg ttcgattccc
     4261 ggctggtgca tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     4321 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     4381 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     4441 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     4501 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     4561 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     4621 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     4681 ctgctataca agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat
     4741 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4801 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4861 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4921 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccacta
     4981 gaaggcctaa ttctagtatg tatgtaagtt aataaaaccc atttttgcgg aaagtagata
     5041 aaaaaaacat tttttttttt tactgcactg gatatcattg aacttatctg atcagtttta
     5101 aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc tctcactcaa
     5161 aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc
     5221 cgtctctttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg
     5281 tcaatgtccg ccttcagttg cattttgtca gcggtttcgt gacgaagctc caagcggttt
     5341 acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt
     5401 gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa
     5461 aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc
     5521 catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg
     5581 atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca
     5641 aaaatcccaa accgcaattg cacaaacaaa tagtgacacg aaacagatta ttctggtagc
     5701 tgttctcgct atataagaca atttttgaga tcatatcatg atcaagacat ctaaaggcat
     5761 tcattttcga ctatattctt ttttacaaaa aatataacaa ccagatattt taagctgatc
     5821 ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact tcggggtact
     5881 ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt
     5941 gcagggtgac agcggagcgg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa
     6001 aaactacggc acgctccggc cacccagtcc gccggaggac tccggttcag ggagcggcca
     6061 actagccgag aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca
     6121 gccgggctcc ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg
     6181 acacataccg gcgcccagga aacatttgct caagaacggt gagtttctat tcgcagtcgg
     6241 ctgatctgtg tgaaatctta ataaagggtc caattaccaa tttgaaactc agtttgcggc
     6301 gtggcctatc cgggcgaact tttggccgtg atgggcagtt ccggtgccgg aaagacgacc
     6361 ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc atccgggatg
     6421 cgactgctca atggccaacc tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc
     6481 cagcaggatg acctctttat cggctcccta acggccaggg aacacctgat tttccaagcc
     6541 atggtgcgga tgccacgaca tctgacctat cggcagcgag tggcccgcgt ggatcaggtg
     6601 atccaggagc tttcgctcag caaatgtcag cacacgatca tcggtgtgcc cggcagggtg
     6661 aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg cctccgaggc tctaaccgat
     6721 ccgccgcttc tgatctgcga tgagcccacc tccggactgg actcctttac cgcccacagc
     6781 gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct gaccattcat
     6841 cagccgtctt ccgagctgtt tgagctcttt gacaagatcc ttctgatggc cgagggcagg
     6901 gtagctttct tgggcactcc cagcgaagcc gtcgacttct tttcctagtg agttcgatgt
     6961 gtttattaag ggtatctagt attacataac atctcaactc ctatccagcg tgggtgccca
     7021 gtgtcctacc aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg
     7081 acgggagatc gagtcccgtg atcggatcgc caagatatgc gacaattttg ccattagcaa
     7141 agtagcccgg gatatggagc agttgttggc caccaaaaat ctggagaagc cactggagca
     7201 gccggagaat gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg
     7261 gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac
     7321 aacggtgagt ggttccagtg gaaacaaatg atataacgct tacaattctt ggaaacaaat
     7381 tcgctagatt ttagatagaa ttgcctgatt ccacaccctt cttagttttt ttcaatgaga
     7441 tgtatagttt atagttttgc agaagataaa taaatttcat ttaactcgcg aatattaatg
     7501 agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg
     7561 ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg gagccatctt cctcttcctg
     7621 accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcatgt ttagaataca
     7681 tttgcatttc aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc
     7741 agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt
     7801 tctgggcaaa acgattgccg aattgccgct ttttctcaca gtgccactgg tcttcacggc
     7861 gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc
     7921 gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag
     7981 ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt
     8041 tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct
     8101 ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc
     8161 gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct
     8221 ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct
     8281 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     8341 gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt
     8401 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
     8461 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
     8521 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
     8581 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
     8641 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
     8701 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
     8761 gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa
     8821 taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc
     8881 tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga
     8941 gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg
     9001 catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat
     9061 aataataaga atacatttaa tttagaaaat gcttggattt cactggaact agaattaatt
     9121 cggctgctgc tctaaacgac gcatttcgta ctccaaagta cgaatttttt ccctcaagct
     9181 cttattttca ttaaacaatg aacaggacct aacgcacagt cacgttattg tttacataaa
     9241 tgattttttt tactattcaa acttactctg tttgtgtact cccactggta tagccttctt
     9301 ttatcttttc tggttcaggc tctatcactt tactaggtac ggcatctgcg ttgagtcgcc
     9361 tccttttaaa tgtctgacct tttgcaggtg cagccttcca ctgcgaatca ttaaagtggg
     9421 tatcacaaat ttgggagttt tcaccaaggc tgcacccaag gctctgctcc cacaattttc
     9481 tcttaatagc acacttcggc acgtgaatta attttactcc agtcacagct ttgcagcaaa
     9541 atttgcaata tttcattttt ttttattcca cgtaagggtt aatgttttca aaaaaaaatt
     9601 cgtccgcaca caacctttcc tctcaacaag caaacgtgca ctgaatttaa gtgtatactt
     9661 cggtaagctt cggctatcga cgggaccacc ttatgttatt tcatcatg
//
