LOCUS       sequence-37231-e        1458 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mitochondria-targeting blue-green emission 
            ratiometric genetically encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-mito-GEM-GECO1
FEATURES             Location/Qualifiers
     source          1..1458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             97..171
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             220..276
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     489..510
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(874..893)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgtctgttc tgactcctct gctgctccgg ggtctcacag gttccgcaag aagactcccc
       61 gtgcctaggg ccaaaattca ttcactgggg gaccccatga gcgtgctcac cccactcctg
      121 ctgcgggggc tgaccggcag cgctaggcgg ctgccagtcc ccagggccaa gatccacagt
      181 ctcggcgatc ccaaggatcc aaccatggtc gactcatcac gtcgtaagtg gaataagaca
      241 ggtcacgcag tcagagctat aggtcggctg agctcaccag agaacgtgta tataaaggcc
      301 gacgagcaga agaacggcat caaggcgtac ttcaagatcc gccacaacat cgagggcggc
      361 ggcgtgcagc tcgcctacca ctaccagcag atcaccccca taggcgacgg ccccgtgctg
      421 ctgcccgaca accactacct gagcgtgcag tccatacttt cgaaagaccc caacgagaag
      481 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      541 gagctgtaca agggtggcag cggtggcatg gtgagcaagg gcgaggagct gttcaccggg
      601 gtggtgccca tccaggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      661 ggcgagggtg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      721 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgtcctacgg cgtgcagtgc
      781 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      841 ggctacatcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      901 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      961 aaggaggacg gcaacatcct ggggcacaag ctggagtaca gcacgcgtga ccaactgact
     1021 gaagagcaga tcgcagaatt taaagaggct ttctccctat ttgacaagga cggggatggg
     1081 acgataacaa ccaaggagct ggggacggtg atgcggtctc tggggcagaa ccccacagaa
     1141 gcagagctgc aggacatgat caatgaagta gatgccgacg gtgacggcac aatcgacttc
     1201 cctgagttcc tgacaatgat ggcacctaaa atgcaggaca cagacagtga agaagaaatt
     1261 agagaagcgt tccgtgtgtt tgataaggac ggcaatggct acatcggcgc agcagagctt
     1321 cgccacgtga tgacaaacct tggagagaag ttaacagatg aagaggttga tgaaatgatc
     1381 agggttgcag acatcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg
     1441 acagcgaagt agaagctt
//
