LOCUS       sequence-372314-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Teige LH, Kumar S, Johansen GM, Wessel O, Vendramin N, Lund M, 
            Rimstad E, Boysen P, Dahle MK
  TITLE     Detection of Salmonid IgM Specific to the Piscine Orthoreovirus 
            Outer Capsid Spike Protein Sigma 1 Using Lipid-Modified Antigens in 
            a Bead-Based Antibody Detection Assay.
  JOURNAL   Front Immunol. 2019 Sep 6;10:2119. doi: 10.3389/fimmu.2019.02119. 
            eCollection 2019.
  PUBMED    31552049
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2019-09-6"; volume: "10:2119. doi"; pages: " 
            10.3389/fimmu.2019.02119. eCollection 2019"
COMMENT     Sequence Label: pcDNA3.1-Hyg-mEGFP-pp4640
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(46..48,49..51,52..762)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLK
                     ERVTAAGITLGMDELYK"
     primer_bind     complement(91..112)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(352..371)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgaattaat acgaactcac tatagggaga cccaagctgg ctagcatggt gagcaagggc
       61 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      121 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      241 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      481 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      541 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      661 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgaa ggagcgcgtg
      721 accgccgccg ggatcactct cggcatggac gagctgtaca agctcgagat gtttcccatg
      781 caattcacca actcagccta tcgccagatg gagcccatgt ttgcaccggg ttcccgagga
      841 caagtacagc cgtaccggcc gcgcactaag cgccgccagg agccgcaagt cggcaacgcc
      901 gccattactg ccctcgcgaa ccagatgagt gcgctccagt tgcaggtagc tggacttgcc
      961 ggccaggcaa gggtggaccg ccgtgggcca agacgtgttc agaagaacaa gcagaagaag
     1021 aagaactctt ccaacggaga aaaacccaaa gagaagaaga agaagccaaa acaacaggag
     1081 aagaaaggaa accgt
//
