LOCUS       sequence-37232-e        1308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of nucleus-targeting red fluorescent 
            genetically encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-NLS-R-GECO
FEATURES             Location/Qualifiers
     source          1..1308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..30
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             70..126
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     822..841
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             865..1308
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 accatggacc caaaaaagaa gcggaaggtc gatcctaaga agaaacgcaa ggtgatggtc
       61 gactcatcac gtcgtaagtg gaataaggca ggtcacgcag tcagagctat aggtcggctg
      121 agctcacccg tggtttccga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc
      181 aagaaggggc tgaggctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac
      241 aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc
      301 gtgtcccaca acgaggacta caccatcgtg gaacagtgcg aacgcgccga gggccgccac
      361 tccaccggcg gcatggacga gctgtacaag ggaggtacag gcgggagtct ggtgagcaag
      421 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      481 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      541 gcctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      601 atcctgtccc ctcagttcat gtacggctcc aaggcctaca ttaagcaccc agccgacatc
      661 cccgactact tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc
      721 gaggacggcg gcattattca cgttaaccag gactcctccc tgcaggacgg cgtattcatc
      781 tacaaggtga agctgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      841 accatgggct gggaggctac gcgtgaccaa ctgactgaag agcagatcgc agaatttaaa
      901 gaggctttct ccctatttga caaggacggg gatgggacga taacaaccaa ggagctgggg
      961 acggtgatgc ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat
     1021 gaagtagatg ccgacggtga cggcacattc gacttccctg agttcctgac gatgatggca
     1081 agaaaaatga atgacacaga cagtgaagag gaaattagag aagcgttccg cgtgtttgat
     1141 aaggacggca atggctacat cggcgcagca gagcttcgcc acgtgatgac agaccttgga
     1201 gagaagttaa cagatgagga ggttgatgaa atgatcaggg tagcagacat cgatggggat
     1261 ggtcaggtaa actacgaaga gtttgtacaa atgatgacag cgaagtag
//
