LOCUS       sequence-372332-        2870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral delivery of Traditional CAR(anti-CD19).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Li HS, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW
  TITLE     High-performance multiplex drug-gated CAR circuits.
  JOURNAL   Cancer Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008.
  PUBMED    36084652
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008."
COMMENT     Sequence Label: pHR-Traditional CAR (anti-CD19)
FEATURES             Location/Qualifiers
     source          1..2870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..1184
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          237..1175
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1132..1152
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1221..1230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2016..2057
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
       61 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      121 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      181 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      241 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      301 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
      361 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
      421 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
      481 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
      541 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
      601 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
      661 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
      721 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
      781 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
      841 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
      901 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
      961 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1021 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     1081 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1141 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggggat ctggagctct
     1201 cgagaattct cacgcgtgcc gccaccatgg ccttaccagt gaccgccttg ctcctgccgc
     1261 tggccttgct gctccacgcc gccaggccgg acatccagat gacacagact acatcctccc
     1321 tgtctgcctc tctgggagac agagtcacca tcagttgcag ggcaagtcag gacattagta
     1381 aatatttaaa ttggtatcag cagaaaccag atggaactgt taaactcctg atctaccata
     1441 catcaagatt acactcagga gtcccatcaa ggttcagtgg cagtgggtct ggaacagatt
     1501 attctctcac cattagcaac ctggagcaag aagatattgc cacttacttt tgccaacagg
     1561 gtaatacgct tccgtacacg ttcggaggcg gcaccaagct ggagatcaca ggtggcggtg
     1621 gctcgggcgg tggtgggtcg ggtggcggcg gatctgaggt gaaactgcag gagtcaggac
     1681 ctggcctggt ggcgccctca cagagcctgt ccgtcacatg cactgtctca ggggtctcat
     1741 tacccgacta tggtgtaagc tggattcgcc agcctccacg aaagggtctg gagtggctgg
     1801 gagtaatatg gggtagtgaa accacatact ataattcagc tctcaaatcc agactgacca
     1861 tcatcaagga caactccaag agccaagttt tcttaaaaat gaacagtctg caaactgatg
     1921 acacagccat ttactactgt gccaaacatt attactacgg tggtagctat gctatggact
     1981 actggggcca aggaacctca gtcaccgtct cctcagggaa gcctatcccg aaccctctgt
     2041 tgggtctcga tagtaccaat ggggccgcaa ccacgacgcc agcgccgcga ccaccaacac
     2101 cggcgcccac catcgcgttg cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg
     2161 cggggggcgc agtgcacacg agggggctgg acttcgcctg tgatttttgg gtgctggtgg
     2221 tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt attattttct
     2281 gggtgaggag taagaggagc aggctcctgc acagtgacta catgaacatg actccccgcc
     2341 gccccgggcc cacccgcaag cattaccagc cctatgcccc accacgcgac ttcgcagcct
     2401 atcgctccaa acggggcaga aagaaactcc tgtatatatt caaacaacca tttatgagac
     2461 cagtacaaac tactcaagag gaagatggct gtagctgccg atttccagaa gaagaagaag
     2521 gaggatgtga actgagagtg aagttcagca ggagcgcaga cgcccccgcg tacaagcagg
     2581 gccagaacca gctctataac gagctcaatc taggacgaag agaggagtac gatgttttgg
     2641 acaagagacg tggccgggac cctgagatgg ggggaaagcc gagaaggaag aaccctcagg
     2701 aaggcctgta caatgaactg cagaaagata agatggcgga ggcctacagt gagattggga
     2761 tgaaaggcga gcgccggagg ggcaaggggc acgatggcct ttaccagggt ctcagtacag
     2821 ccaccaagga cacctacgac gcccttcaca tgcaggccct gccccctcgc
//
