LOCUS       sequence-37234-e        1347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-GEM-GECO1
FEATURES             Location/Qualifiers
     source          1..1347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..60
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(64..72,73..87)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             115..171
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     384..405
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(769..788)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
       61 cgggatctgt acgacgatga tgataaggat ctcgccacaa tggtcgactc atcacgtcgt
      121 aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagctc accagagaac
      181 gtgtatataa aggccgacga gcagaagaac ggcatcaagg cgtacttcaa gatccgccac
      241 aacatcgagg gcggcggcgt gcagctcgcc taccactacc agcagatcac ccccataggc
      301 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccat actttcgaaa
      361 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      421 actctcggca tggacgagct gtacaagggt ggcagcggtg gcatggtgag caagggcgag
      481 gagctgttca ccggggtggt gcccatccag gtcgagctgg acggcgacgt aaacggccac
      541 aagttcagcg tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag
      601 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc
      661 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      721 tccgccatgc ccgaaggcta catccaggag cgcaccatct tcttcaagga cgacggcaac
      781 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      841 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacagcacg
      901 cgtgaccaac tgactgaaga gcagatcgca gaatttaaag aggctttctc cctatttgac
      961 aaggacgggg atgggacgat aacaaccaag gagctgggga cggtgatgcg gtctctgggg
     1021 cagaacccca cagaagcaga gctgcaggac atgatcaatg aagtagatgc cgacggtgac
     1081 ggcacaatcg acttccctga gttcctgaca atgatggcac ctaaaatgca ggacacagac
     1141 agtgaagaag aaattagaga agcgttccgt gtgtttgata aggacggcaa tggctacatc
     1201 ggcgcagcag agcttcgcca cgtgatgaca aaccttggag agaagttaac agatgaagag
     1261 gttgatgaaa tgatcagggt agcagacatc gatggngang gtcaggtaaa ctacgaagag
     1321 tttgtacaaa tgatgacagc gaagtag
//
