LOCUS       sequence-372340-        4944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A5_STOP
FEATURES             Location/Qualifiers
     source          1..4944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2501..2600)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2617..2636)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2618..2636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2641..2657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2641..2657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2759..2778)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2770..3579
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2837..2856)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3726..4314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4215..4234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4468..4485
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4644..4671)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4763..4849)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4898..4920
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4912..4929
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4913..4929
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ctgctccgcc
      121 cccaaggaga tgaacaagct gccagccaat tctccagagg cagcagcagc acagggacac
      181 ccagatggac cttgtgcacc acggacctct ccagagcagg agctgcctgc agcagcagca
      241 ccacctccac cacgggtgcc tagatccgcc tctacaggcg cccagacctt ccagagcgcc
      301 gacgcaaggg catgcgaggc cgagagacca ggcgtgggca gctgtaagct gagctcccca
      361 agagcacagg cagcatccgc cgccctgagg gatctgcgcg aggcccaggg cgcccaggcc
      421 tcccctccac ccggctctag cggaccagga aacgcactgc actgcaagat cccatttctg
      481 aggggaccag agggcgacgc aaacgtgagc gtgggcaagg gcacactgga gcgcaacaat
      541 acccctgtgg tgggctgggt gaacatgagc cagtccaccg tggtgctggc cacagatggc
      601 atcacctctg tgctgccagg aagcgtggca accgtggcaa cacaggagga cgagcagggc
      661 gatgagaaca aggcccgggg caattggtcc tctaagctgg acttcatcct gtccatggtg
      721 ggatacgcag tgggactggg aaacgtgtgg agattcccat atctggcctt tcagaatggc
      781 ggcggcgcct tcctgatccc ttacctgatg atgctggccc tggcaggact gccaatcttc
      841 tttctggagg tgagcctggg acagtttgca tcccagggac ccgtgagcgt gtggaaggca
      901 atccctgccc tgcagggatg cggaatcgca atgctgatca tctccgtgct gatcgccatc
      961 tactataacg tgatcatctg ttacaccctg ttctatctgt ttgcctcttt cgtgagcgtg
     1021 ctgccctggg gcagctgcaa caatccttgg aatacaccag agtgtaagga taagaccaag
     1081 ctgctgctgg acagctgcgt gatctccgat cacccaaaga tccagatcaa gaactccaca
     1141 ttctgtatga ccgcctaccc caacgtgaca atggtgaatt tcacctctca ggccaacaag
     1201 accttcgtga gcggcagcga ggagtacttt aagtatttcg tgctgaagat cagcgccggc
     1261 atcgagtacc ctggagagat caggtggcca ctggccctgt gcctgttcct ggcctgggtc
     1321 atcgtgtacg cctctctggc caagggcatc aagaccagcg gcaaggtggt gtacttcacc
     1381 gccacatttc cctatgtggt gctggtcatc ctgctgatca gaggagtgac actgcctgga
     1441 gcaggagcag gaatctggta ctttatcacc ccaaagtggg agaagctgac cgacgccaca
     1501 gtgtggaagg atgccgccac acagatcttc ttttccctgt ctgccgcatg gggaggactg
     1561 atcaccctga gctcctacaa caagttccac aacaattgct atagggacac actgatcgtg
     1621 acctgtacaa attccgccac ctctatcttt gccggcttcg tgatcttttc cgtgatcggc
     1681 tttatggcca acgagaggaa ggtgaacatc gagaatgtgg ccgatcaggg cccaggcatc
     1741 gccttcgtgg tgtatcctga ggccctgacc aggctgccac tgagcccttt ttgggccatc
     1801 atcttctttc tgatgctgct gacactgggc ctggacacca tgttcgccac catcgagaca
     1861 atcgtgacca gcatctccga tgagttccct aagtacctgc ggacacacaa gccagtgttt
     1921 accctgggct gctgtatctg cttctttatc atgggcttcc ctatgatcac acagggcggc
     1981 atctatatgt ttcagctggt ggacacctac gccgcctctt atgccctggt catcatcgcc
     2041 atcttcgagc tggtgggcat cagctacgtg tatggcctgc agaggttttg tgaggatatc
     2101 gagatgatga tcggcttcca gcccaacatc ttttggaagg tgtgctgggc cttcgtgacc
     2161 cctacaatcc tgaccttcat cctgtgcttc agcttttacc agtgggagcc catgacctat
     2221 ggcagctacc gctatcctaa ttggtccatg gtgctgggct ggctgatgct ggcctgcagc
     2281 gtgatctgga tccccatcat gttcgtgatc aagatgcacc tggcccctgg caggtttatc
     2341 gagcgcctga agctggtgtg ctcccctcag ccagactggg gacccttcct ggcacagcac
     2401 aggggagaga gatacaagaa tatgatcgac ccactgggca catctagcct gggcctgaag
     2461 ctgcccgtga aggatctgga gctgggcacc cagtgctaag acccagcttt cttgtacaaa
     2521 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     2581 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     2641 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     2701 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     2761 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     2821 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2881 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2941 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     3001 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     3061 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     3121 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     3181 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     3241 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     3301 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     3361 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     3421 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     3481 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     3541 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     3601 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     3661 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3721 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3781 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3841 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3901 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3961 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4021 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4081 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4141 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4201 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4261 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4321 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4381 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4441 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4501 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4561 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     4621 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4681 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4741 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4801 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4861 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4921 acggccagtc ttaagctcgg gccc
//
