LOCUS       sequence-372341-        4098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC9B1_STOP
FEATURES             Location/Qualifiers
     source          1..4098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1655..1754)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1771..1790)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1772..1790)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1795..1811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1795..1811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1913..1932)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1924..2733
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1991..2010)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2880..3468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3369..3388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3622..3639
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3798..3825)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3917..4003)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4052..4074
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4066..4083
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4067..4083
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgca caccacagag
      121 agcaagaacg agcacctgga ggacgagaat tttcagacct ccaccacacc acagtctctg
      181 atcgatccca acaataccgc ccaggaggag accaagacag tgctgtctga cacagaggag
      241 atcaagcctc agaccaagaa ggagacatac atcagctgcc cactgcgggg cgtgctgaac
      301 gtgatcatca ccaatggcgt gatcctgttc gtgatctggt gtatgacatg gtctatcctg
      361 ggaagcgagg ccctgccagg aggaaacctg ttcggcctgt ttatcatctt ctatagcgcc
      421 atcatcggcg gcaagatcct gcagctgatc aggatcccac tggtgccacc tctgccacca
      481 ctgctgggaa tgctgctggc aggctttacc atccgcaacg tgcctttcat caatgagcac
      541 gtgcacgtgc caaatacctg gagctccatc ctgaggtcca tcgccctgac aatcatcctg
      601 atcagggcag gactgggact ggatccacag gccctgaggc acctgaaggt ggtgtgcttt
      661 agactggccg tgggaccttg tctgatggag gcatccgccg cagccgtgtt ttctcacttc
      721 atcatgaagt tcccttggca gtgggccttt ctgctgggct tcgtgctggg agccgtgtct
      781 ccagcagtgg tggtgcctta catgatggtg ctgcaggaga acggctatgg cgtggaggag
      841 ggcatcccaa ccctgctgat ggccgcctct agcatggacg atatcctggc catcaccggc
      901 tttaacacat gcctgtccat cgtgttctcc tctggcggca tcctgaacaa tgccatcgcc
      961 tctatcagga acgtgtgcat cagcctgctg gccggcatcg tgctgggctt ctttgtgcgg
     1021 tactttccca gcgaggacca gaagaagctg accctgaaga gaggcttcct ggtgctgaca
     1081 atgtgcgtga gcgccgtgct gggatcccag aggatcggac tgcacggctc cggcggcctg
     1141 tgcaccctgg tgctgagctt tatcgccggc acaaagtggt cccaggagaa gatgaaggtg
     1201 cagaagatca tcaccacagt gtgggatatc tttcagcctc tgctgttcgg actggtggga
     1261 gcagaggtga gcgtgagctc cctggagtcc aacatcgtgg gcatctctgt ggccaccctg
     1321 agcctggccc tgtgcgtgag aatcctgacc acatatctgc tgatgtgctt tgccggcttc
     1381 tcctttaagg agaagatctt catcgccctg gcctggatgc caaaggccac cgtgcaggcc
     1441 gtgctgggac cactggccct ggagacagca cgggtgtctg cccctcacct ggagccatac
     1501 gccaaggacg tgatgaccgt ggccttcctg gccatcctga tcacagcccc caatggcgcc
     1561 ctgctgatgg gaatcctggg accaaagatg ctgaccagac actatgatcc ttccaagatc
     1621 aagctgcagc tgtctacact ggagcaccac taagacccag ctttcttgta caaagttggc
     1681 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1741 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1801 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1861 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     1921 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     1981 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2041 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2101 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2161 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2221 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2281 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2341 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2401 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2461 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2521 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2581 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2641 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2701 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2761 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     2821 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3541 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3601 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3661 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3721 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3781 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3841 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3901 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     3961 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4021 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4081 agtcttaagc tcgggccc
//
