LOCUS       sequence-372360-       11078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CAMKK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11078)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CAMKK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..11078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..69
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             138..737
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(138..157)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     634..654
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             875..1108
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1180..1301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1217..1236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1271..1290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1341..1476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1496..1515)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1497..1515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1525..1542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1525..1541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1534..1556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1683..2138
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1770..1789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1980..2001
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2164..2268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2269..2337,2338..3129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3300..3888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3789..3808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4042..4059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4176..4197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4212..4217,4218..4235,4236..4242)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4250..4266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4255..4277
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4274..4290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4274..4290
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4309..4329
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4311..4329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4357..4583
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4584..4764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4811..4936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5429..5662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5847..5891
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6158..6274
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6308..6328
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6328..7539
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7376..7395
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7543..7563)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7564..7573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7576..8346
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8353..8394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8395..8419
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10052..10076)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10106..10694
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10159..10179)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10577..10588)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10708..11078,1..117)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10743..10766)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
       61 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
      121 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
      181 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
      241 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
      301 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
      361 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
      421 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
      481 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
      541 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
      601 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
      661 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
      721 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
      781 gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta
      841 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga
      901 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
      961 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1021 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1081 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     1141 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     1201 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1261 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     1321 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     1381 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     1441 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     1501 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1561 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1621 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1681 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1741 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1801 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1861 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1921 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1981 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     2041 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2101 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     2161 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2221 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2281 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2341 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2401 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2461 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2521 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2581 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2641 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2701 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2761 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2821 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2881 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2941 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3001 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3061 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3121 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3421 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3661 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3721 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3781 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3841 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3901 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3961 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4021 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     4201 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4261 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     4321 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     4381 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     4441 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     4501 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     4561 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     4621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4741 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     4801 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     4861 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     4921 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     4981 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     5041 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     5101 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     5161 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     5221 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     5281 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     5341 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     5401 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     5461 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     5521 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     5581 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     5641 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     5701 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     5761 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     5821 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     5881 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     5941 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     6001 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     6061 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     6121 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
     6181 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     6241 aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg gaattctgca
     6301 gatatcacaa ctttgtatag aaaagttggc ctccgcgccg ggttttggcg cctcccgcgg
     6361 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
     6421 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
     6481 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
     6541 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
     6601 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
     6661 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
     6721 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
     6781 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
     6841 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
     6901 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
     6961 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
     7021 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
     7081 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
     7141 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
     7201 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
     7261 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     7321 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     7381 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     7441 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     7501 tccgctaaat tctggccgtt tttggctttt ttgttagacg gccaactttt gtatacaaag
     7561 tgggccacca tgggaatccc gctgctgaac gctaaacaga ttctgggaca gctggacggc
     7621 gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct ggatcgaatc
     7681 ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg gagaggaagc
     7741 agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat gttttggcgc
     7801 ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt catggcagaa
     7861 gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga cctgtatctg
     7921 caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg cgatgctgca
     7981 cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga aagcgtggtc
     8041 aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa tactctggcc
     8101 gctatgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga aggcctggct
     8161 ccatatctgt cacggtggga gaagctggat aacttcatca atagacccgt gaagctgatc
     8221 attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg agccctgctg
     8281 ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct gcggtctgcc
     8341 gaaaaggcta gcggcaagcc catccccaac cccctgctgg gcctggacag caccacaagt
     8401 ttgtacaaaa aagcaggctc caccatgtca tcatgtgtct ctagccagcc cagcagcaac
     8461 cgggccgccc cccaggatga gctggggggc aggggcagca gcagcagcga aagccagaag
     8521 ccctgtgagg ccctgcgggg cctctcatcc ttgagcatcc acctgggcat ggagtccttc
     8581 attgtggtca ccgagtgtga gccgggctgt gctgtggacc tcggcttggc gcgggaccgg
     8641 cccctggagg ccgatggcca agaggtcccc cttgactcct ccgggtccca ggcccggccc
     8701 cacctctccg gtcgcaagct gtctctgcaa gagcggtccc agggtgggct ggcagccggt
     8761 ggcagcctgg acatgaacgg acgctgcatc tgcccgtccc tgccctactc acccgtcagc
     8821 tccccgcagt cctcgcctcg gctgccccgg cggccgacag tggagtctca ccacgtctcc
     8881 atcacgggta tgcaggactg tgtgcagctg aatcagtata ccctgaagga tgaaattgga
     8941 aagggctcct atggtgtcgt caagttggcc tacaatgaaa atgacaatac ctactatgca
     9001 atgaaggtgc tgtccaaaaa gaagctgatc cggcaggccg gctttccacg tcgccctcca
     9061 ccccgaggca cccggccagc tcctggaggc tgcatccagc ccaggggccc cattgagcag
     9121 gtgtaccagg aaattgccat cctcaagaag ctggaccacc ccaatgtggt gaagctggtg
     9181 gaggtcctgg atgaccccaa tgaggaccat ctgtacatgg tgttcgaact ggtcaaccaa
     9241 gggcccgtga tggaagtgcc caccctcaaa ccactctctg aagaccaggc ccgtttctac
     9301 ttccaggatc tgatcaaagg catcgagtac ttacactacc agaagatcat ccaccgtgac
     9361 atcaaacctt ccaacctcct ggtcggagaa gatgggcaca tcaagatcgc tgactttggt
     9421 gtgagcaatg aattcaaggg cagtgacgcg ctcctctcca acaccgtggg cacgcccgcc
     9481 ttcatggcac ccgagtcgct ctctgagacc cgcaagatct tctctgggaa ggccttggat
     9541 gtttgggcca tgggtgtgac actatactgc tttgtctttg gccagtgccc attcatggac
     9601 gagcggatca tgtgtttaca cagtaagatc aagagtcagg ccctggaatt tccagaccag
     9661 cccgacatag ctgaggactt gaaggacctg atcacccgta tgctggacaa gaaccccgag
     9721 tcgaggatcg tggtgccgga aatcaagctg cacccctggg tcacgaggca tggggcggag
     9781 ccgttgccgt cggaggatga gaactgcacg ctggtcgaag tgactgaaga ggaggtcgag
     9841 aactcagtca aacacattcc cagcttggca accgtgatcc tggtgaagac catgatacgt
     9901 aaacgctcct ttgggaaccc attcgagggc agccggcggg aggaacgctc actgtcagcg
     9961 cctggaaact tgctcacgaa gcaaggcagc gaagacaacc tccagggcac cgacccgccc
    10021 cccgtggggg aggaggaagt gctcttgtag gacccagctt tcttgtacaa agtggtgata
    10081 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt
    10141 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
    10201 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
    10261 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
    10321 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
    10381 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
    10441 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
    10501 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
    10561 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
    10621 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
    10681 ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca aggcagtctg
    10741 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
    10801 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
    10861 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
    10921 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
    10981 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
    11041 ctttctgggc tcagaggctg ggaaggggtg ggtccggg
//
