LOCUS       sequence-372366-       11316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of HIPK4 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11316)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-HIPK4_IDG-K
FEATURES             Location/Qualifiers
     source          1..11316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     439..459
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             680..913
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    985..1106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1022..1041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1076..1095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1146..1281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1301..1320)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1302..1320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1330..1347)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1330..1346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1339..1361)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1488..1943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1575..1594)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1785..1806
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1969..2073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2074..2142,2143..2934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3105..3693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3594..3613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3847..3864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3981..4002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4017..4022,4023..4040,4041..4047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4055..4071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4060..4082
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4079..4095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4079..4095
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4114..4134
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4116..4134
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4162..4388
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4389..4569
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4616..4741
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5234..5467
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5652..5696
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5963..6079
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6113..6133
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6133..7344
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7181..7200
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7348..7368)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7369..7378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7381..8151
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8158..8199
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8200..8224
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9603..9617
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     protein_bind    complement(10082..10106)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10136..10724
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10189..10209)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10607..10618)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10738..11238
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     11171..11190
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(11259..11316,1..542)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11259..11278)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
       61 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
      121 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
      181 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
      241 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
      301 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
      361 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
      421 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
      481 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
      541 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc
      601 gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
      661 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt
      721 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      781 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      841 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      901 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
      961 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     1021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     1081 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     1141 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1201 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     1261 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     1321 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1381 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1441 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     1501 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1561 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1621 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1681 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1741 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1801 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1861 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1921 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     1981 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2041 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2101 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2161 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2221 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2281 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2341 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2401 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2461 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2521 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2581 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2641 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2701 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2761 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2821 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2881 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2941 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3001 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3241 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3721 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3781 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3841 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3901 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3961 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4021 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4081 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     4141 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     4201 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     4261 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     4321 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     4381 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4441 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4501 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4561 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     4621 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     4681 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     4741 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     4801 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     4861 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     4921 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     4981 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     5041 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     5101 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     5161 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     5221 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     5281 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     5341 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     5401 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     5461 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     5521 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     5581 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     5641 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     5701 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     5761 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     5821 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     5881 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     5941 gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag
     6001 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     6061 ttacaaaatt caaaatttta tcgtccagtg tggtggaatt ctgcagatat cacaactttg
     6121 tatagaaaag ttggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     6181 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     6241 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     6301 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     6361 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     6421 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     6481 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     6541 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     6601 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     6661 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     6721 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     6781 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     6841 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     6901 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     6961 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     7021 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     7081 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     7141 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     7201 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     7261 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     7321 ccgtttttgg cttttttgtt agacggccaa cttttgtata caaagtgggc caccatggga
     7381 atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc
     7441 ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt
     7501 ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg
     7561 ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca
     7621 gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg
     7681 ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg
     7741 gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc
     7801 gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc
     7861 acactgcagg aagcagggat taacctggac aggaatactc tggccgctat gctgatccga
     7921 gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg
     7981 tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag
     8041 attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga
     8101 gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc
     8161 aagcccatcc ccaaccccct gctgggcctg gacagcacca caagtttgta caaaaaagca
     8221 ggctccacca tgtccaccat ccagtcggag actgactgct acgacatcat cgaggtcttg
     8281 ggcaagggga ccttcgggga ggtagccaag ggctggcggc ggagcacggg cgagatggtg
     8341 gccatcaaga tcctcaagaa tgacgcctac cgcaaccgca tcatcaaaaa cgagctgaag
     8401 ctgctgcact gcatgcgagg cctagaccct gaagaggccc acgtcatccg cttccttgag
     8461 ttcttccatg acgccctcaa gttctacctg gtctttgagc tgctggagca aaaccttttc
     8521 gagttccaga aggagaacaa cttcgcgccc ctccccgccc gccacatccg tacagtcacc
     8581 ctgcaggtgc tcacagccct ggcccggctc aaggagctgg ctatcatcca cgctgatctc
     8641 aagcctgaga acatcatgct ggtggaccag acccgctgcc ccttcagggt caaggtgatt
     8701 gacttcggat ccgccagcat tttcagcgag gtgcgctacg tgaaggagcc atacatccag
     8761 tcgcgcttct accgggcccc tgagatcctg ctggggctgc ccttctgcga gaaggtggac
     8821 gtgtggtccc tgggctgcgt catggctgag ctgcacctgg gctggcctct ctaccccggc
     8881 aacaacgagt acgaccaggt gcgctacatc tgcgaaaccc agggcctgcc caagccacac
     8941 ctgttgcacg ccgcctgcaa ggcccaccac ttcttcaagc gcaaccccca ccctgacgct
     9001 gccaacccct ggcagctcaa gtcctcggct gactacctgg ccgagacgaa ggtgcgccca
     9061 ttggagcgcc gcaagtatat gctcaagtcg ttggaccaga ttgagacagt gaatggtggc
     9121 agtgtggcca gtcggctaac cttccctgac cgggaggcgc tggcggagca cgccgacctc
     9181 aagagcatgg tggagctgat caagcgcatg ctgacctggg agtcacacga acgcatcagc
     9241 cccagtgctg ccctgcgcca ccccttcgtg tccatgcagc agctgcgcag tgcccacgag
     9301 accacccact actaccagct ctcgctgcgc agctaccgcc tctcgctgca agtggagggg
     9361 aagcccccca cgcccgtcgt gaccgcagaa gatgggaccc cctactactg tctggctgag
     9421 gagaaggagg ctgcgggtat gggcagtgtg gccggcagca gccccttctt ccgagaggag
     9481 aaggcaccag gtatgcaaag agccatcgac cagctggatg acctgagtct gcaggaggct
     9541 gggcatgggc tgtggggtga gacctgcacc aatgcggtct ccgacatgat ggtccccctc
     9601 aaggcagcca tcactggcca ccatgtgccc gactcgggcc ctgagcccat cctggccttc
     9661 tacagcagcc gcctggcagg ccgccacaag gcccgcaagc cacctgcggg ttccaagtcc
     9721 gactccaact tcagcaacct cattcggctg agccaggtct cgcctgagga tgacaggccc
     9781 tgccggggca gcagctggga ggaaggagag tatctcgggg cctctgctga gccactggcc
     9841 atcctgcagc gagatgagga tgggcccaac attgacaaca tgaccatgga agctgagagg
     9901 ccagaccctg agctcttcga ccccagcagc tgtcctggag aatggctgag tgagccagac
     9961 tgcaccctgg agagcgtcag gggcccacgg gctcaggggc tcccaccccg ccgctcccac
    10021 cagcatggtc caccccgggg ggccaccagc ttcctccagc atgtcaccgg gcaccactag
    10081 gacccagctt tcttgtacaa agtggtgata tccagcacag tggcggccgc tcgacaatca
    10141 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
    10201 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
    10261 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    10321 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    10381 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    10441 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    10501 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
    10561 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    10621 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    10681 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctaccggg
    10741 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
    10801 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
    10861 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
    10921 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
    10981 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
    11041 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
    11101 ggtccggggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
    11161 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
    11221 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
    11281 tgcgcctcgc cacccgcgac gacgtcccca gggccg
//
