LOCUS       sequence-372386-       10119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human ERBB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_ERBB2
FEATURES             Location/Qualifiers
     source          1..10119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1999..2018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2190..2569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2570..2773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2724..2744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2775..2793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2796..2814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2824..2848
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6811..6835)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6882..6905
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6942..6965
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6996..7022
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6996..7022
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7023..7370,7371..7373,7374..7982)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8026..8043)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8032..8256
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8389..8408)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8540..8587
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8595..9617
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8595..9617
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9747..9868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9784..9803)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9838..9857
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9917..9933)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9917..9933)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9930..9952)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9941..9957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9965..9971,9972..9989,9990..9995))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10010..10031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1981 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2041 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2101 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2161 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2761 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2821 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2881 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg
     2941 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     3001 acaaaaaagc agaagggccg tcaaggccca ccatggagct ggcggccttg tgccgctggg
     3061 ggctcctcct cgccctcttg ccccccggag ccgcgagcac ccaagtgtgc accggcacag
     3121 acatgaagct gcggctccct gccagtcccg agacccacct ggacatgctc cgccacctct
     3181 accagggctg ccaggtggtg cagggaaacc tggaactcac ctacctgccc accaatgcca
     3241 gcctgtcctt cctgcaggat atccaggagg tgcagggcta cgtgctcatc gctcacaacc
     3301 aagtgaggca ggtcccactg cagaggctgc ggattgtgcg aggcacccag ctctttgagg
     3361 acaactatgc cctggccgtg ctagacaatg gagacccgct gaacaatacc acccctgtca
     3421 caggggcctc cccaggaggc ctgcgggagc tgcagcttcg aagcctcaca gagatcttga
     3481 aaggaggggt cttgatccag cggaaccccc agctctgcta ccaggacacg attttgtgga
     3541 aggacatctt ccacaagaac aaccagctgg ctctcacact gatagacacc aaccgctctc
     3601 gggcctgcca cccctgttct ccgatgtgta agggctcccg ctgctgggga gagagttctg
     3661 aggattgtca gagcctgacg cgcactgtct gtgccggtgg ctgtgcccgc tgcaaggggc
     3721 cactgcccac tgactgctgc catgagcagt gtgctgccgg ctgcacgggc cccaagcact
     3781 ctgactgcct ggcctgcctc cacttcaacc acagtggcat ctgtgagctg cactgcccag
     3841 ccctggtcac ctacaacaca gacacgtttg agtccatgcc caatcccgag ggccggtata
     3901 cattcggcgc cagctgtgtg actgcctgtc cctacaacta cctttctacg gacgtgggat
     3961 cctgcaccct cgtctgcccc ctgcacaacc aagaggtgac agcagaggat ggaacacagc
     4021 ggtgtgagaa gtgcagcaag ccctgtgccc gagtgtgcta tggtctgggc atggagcact
     4081 tgcgagaggt gagggcagtt accagtgcca atatccagga gtttgctggc tgcaagaaga
     4141 tctttgggag cctggcattt ctgccggaga gctttgatgg ggacccagcc tccaacactg
     4201 ccccgctcca gccagagcag ctccaagtgt ttgagactct ggaagagatc acaggttacc
     4261 tatacatctc agcatggccg gacagcctgc ctgacctcag cgtcttccag aacctgcaag
     4321 taatccgggg acgaattctg cacaatggcg cctactcgct gaccctgcaa gggctgggca
     4381 tcagctggct ggggctgcgc tcactgaggg aactgggcag tggactggcc ctcatccacc
     4441 ataacaccca cctctgcttc gtgcacacgg tgccctggga ccagctcttt cggaacccgc
     4501 accaagctct gctccacact gccaaccggc cagaggacga gtgtgtgggc gagggcctgg
     4561 cctgccacca gctgtgcgcc cgagggcact gctggggtcc agggcccacc cagtgtgtca
     4621 actgcagcca gttccttcgg ggccaggagt gcgtggagga atgccgagta ctgcaggggc
     4681 tccccaggga gtatgtgaat gccaggcact gtttgccgtg ccaccctgag tgtcagcccc
     4741 agaatggctc agtgacctgt tttggaccgg aggctgacca gtgtgtggcc tgtgcccact
     4801 ataaggaccc tcccttctgc gtggcccgct gccccagcgg tgtgaaacct gacctctcct
     4861 acatgcccat ctggaagttt ccagatgagg agggcgcatg ccagccttgc cccatcaact
     4921 gcacccactc ctgtgtggac ctggatgaca agggctgccc cgccgagcag agagccagcc
     4981 ctctgacgtc catcatctct gcggtggttg gcattctgct ggtcgtggtc ttgggggtgg
     5041 tctttgggat cctcatcaag cgacggcagc agaagatccg gaagtacacg atgcggagac
     5101 tgctgcagga aacggagctg gtggagccgc tgacacctag cggagcgatg cccaaccagg
     5161 cgcagatgcg gatcctgaaa gagacggagc tgaggaaggt gaaggtgctt ggatctggcg
     5221 cttttggcac agtctacaag ggcatctgga tccctgatgg ggagaatgtg aaaattccag
     5281 tggccatcaa agtgttgagg gaaaacacat cccccaaagc caacaaagaa atcttagacg
     5341 aagcatacgt gatggctggt gtgggctccc catatgtctc ccgccttctg ggcatctgcc
     5401 tgacatccac ggtgcagctg gtgacacagc ttatgcccta tggctgcctc ttagaccatg
     5461 tccgggaaaa ccgcggacgc ctgggctccc aggacctgct gaactggtgt atgcagattg
     5521 ccaaggggat gagctacctg gaggatgtgc ggctcgtaca cagggacttg gccgctcgga
     5581 acgtgctggt caagagtccc aaccatgtca aaattacaga cttcgggctg gctcggctgc
     5641 tggacattga cgagacagag taccatgcag atgggggcaa ggtgcccatc aagtggatgg
     5701 cgctggagtc cattctccgc cggcggttca cccaccagag tgatgtgtgg agttatggtg
     5761 tgactgtgtg ggagctgatg acttttgggg ccaaacctta cgatgggatc ccagcccggg
     5821 agatccctga cctgctggaa aagggggagc ggctgcccca gccccccatc tgcaccattg
     5881 atgtctacat gatcatggtc aaatgttgga tgattgactc tgaatgtcgg ccaagattcc
     5941 gggagttggt gtctgaattc tcccgcatgg ccagggaccc ccagcgcttt gtggtcatcc
     6001 agaatgagga cttgggccca gccagtccct tggacagcac cttctaccgc tcactgctgg
     6061 aggacgatga catgggggac ctggtggatg ctgaggagta tctggtaccc cagcagggct
     6121 tcttctgtcc agaccctgcc ccgggcgctg ggggcatggt ccaccacagg caccgcagct
     6181 catctaccag gagtggcggt ggggacctga cactagggct ggagccctct gaagaggagg
     6241 cccccaggtc tccactggca ccctccgaag gggctggctc cgatgtattt gatggtgacc
     6301 tgggaatggg ggcagccaag gggctgcaaa gcctccccac acatgacccc agccctctac
     6361 agcggtacag tgaggacccc acagtacccc tgccctctga gactgatggc tacgttgccc
     6421 ccctgacctg cagcccccag cctgaatatg tgaaccagcc agatgttcgg ccccagcccc
     6481 cttcgccccg agagggccct ctgcctgctg cccgacctgc tggtgccact ctggaaaggc
     6541 ccaagactct ctccccaggg aagaatgggg tcgtcaaaga cgtttttgcc tttgggggtg
     6601 ccgtggagaa ccccgagtac ttgacacccc agggaggagc tgcccctcag ccccaccctc
     6661 ctcctgcctt cagcccagcc ttcgacaacc tctattactg ggaccaggac ccaccagagc
     6721 ggggggctcc acccagcacc ttcaaaggga cacctacggc agagaaccca gagtacctgg
     6781 gtctggacgt gccagtgtca ggcctcatgg gcccagcttt cttgtacaaa gtggttgata
     6841 tcaaggccgc cgacatcacc agcctgtaca agaaagtggg gtggagccat ccccagttcg
     6901 agaagggcgg aggatctggc ggcggaagcg gcggaggaag ctggtcccac cctcagtttg
     6961 agaaaggcac cgagctgggc agcaccatgg ccagctaccc ctacgacgtg cccgactacg
     7021 ccaaggacaa caccgtgccc ctgaagctga tcgccctgct ggccaacggc gagttccact
     7081 ctggcgagca gctgggcgag acactgggca tgagcagagc cgccatcaac aagcacatcc
     7141 agaccctgcg ggactggggc gtggacgtgt tcacagtgcc cggcaagggc tacagcctgc
     7201 ccgagcccat ccagctgctg aacgccaagc agatcctggg ccagctggat ggcggatctg
     7261 tggccgtgct gcccgtgatc gacagcacca accagtatct gctcgaccgg atcggcgagc
     7321 tgaagtccgg cgacgcctgt atcgccgagt accagcaggc cggcagaggc ggcaggggca
     7381 gaaagtggtt cagccccttc ggcgccaacc tgtacctgag catgttctgg agactggaac
     7441 agggcccagc cgccgctatt ggcctgagcc tggtcatcgg catcgtgatg gccgaggtgc
     7501 tgcggaagct gggcgccgac aaagtgcgcg tgaagtggcc caacgacctg tatctgcagg
     7561 accggaagct ggccggcatc ctggtggaac tgaccggcaa aacaggcgac gccgcccaga
     7621 tcgtgatcgg cgctggcatc aacatggcca tgcggcgggt ggaagagagc gtggtcaacc
     7681 agggctggat caccctgcag gaagccggca tcaacctgga ccggaacacc ctggccgcca
     7741 tgctgatcag agagctgaga gccgccctgg aactgttcga gcaggaaggc ctggccccct
     7801 acctgagcag atgggagaag ctggacaact tcatcaaccg gcccgtgaaa ctgatcatcg
     7861 gcgacaaaga gatcttcggc atcagccggg gcatcgacaa gcagggggcc ctgctcctgg
     7921 aacaggacgg catcatcaag ccctggatgg gcggcgagat cagcctgcgg agcgccgaga
     7981 agtgatgact cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     8041 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8101 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8161 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8221 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     8281 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8341 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8401 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8461 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8521 gtgatggttc acgtacctag aagttcctat tccgaagttc ctattctcta gaaagtatag
     8581 gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     8641 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     8701 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     8761 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     8821 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     8881 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     8941 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     9001 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     9061 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     9121 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     9181 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     9241 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     9301 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     9361 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     9421 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     9481 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     9541 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     9601 cgagggcaaa ggaatagcac gtactacgag atttcgattc caccgccgcc ttctatgaaa
     9661 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     9721 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     9781 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9841 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     9901 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9961 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    10021 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    10081 agctgcatta atgaatcggc caacgcgcgg ggagaggcg
//
