LOCUS       sequence-37239-e        1353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-R-GECO1
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..60
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(64..72,73..87)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             115..171
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     867..886
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             910..1353
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
       61 cgggatctgt acgacgatga tgataaggat ctcgccacaa tggtcgactc ttcacgtcgt
      121 aagtggaata aggcaggtca cgcagtcaga gctataggtc ggctgagctc acccgtggtt
      181 tccgagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa ggggctgagg
      241 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      301 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag
      361 gactacacca tcgtggaaca gtgcgaacgc gccgagggcc gccactccac cggcggcatg
      421 gacgagctgt acaagggagg tacaggcggg agtctggtga gcaagggcga ggaggataac
      481 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      541 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      601 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      661 ttcatgtacg gctccaaggc ctacattaag cacccagccg acatccccga ctacttcaag
      721 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      781 attcacgtta accaggactc ctccctgcag gacggcgtat tcatctacaa ggtgaagctg
      841 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      901 gctacgcgtg accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta
      961 tttgacaagg acggggatgg gacgataaca accaaggagc tggggacggt gatgcggtct
     1021 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     1081 ggtgacggca cattcgactt ccctgagttc ctgacgatga tggcaagaaa aatgaatgac
     1141 acagacagtg aagaggaaat tagagaagcg ttccgcgtgt ttgataagga cggcaatggc
     1201 tacatcggcg cagcagagct tcgccacgtg atgacagacc ttggagagaa gttaacagat
     1261 gaggaggttg atgaaatgat cagggtagca gacatcgatg gggatggtca ggtaaactac
     1321 gaagagtttg tacaaatgat gacagcgaag tag
//
