LOCUS       sequence-372391-        9159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human ROR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_ROR2
FEATURES             Location/Qualifiers
     source          1..9159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1981..2000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2172..2551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2552..2755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2706..2726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2778..2796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2806..2830
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2973..2997
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5833..5857)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5904..5927
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5964..5987
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6018..6044
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6018..6044
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6045..6392,6393..6395,6396..7004)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7048..7065)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7054..7278
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7411..7430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7562..7609
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7617..8639
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7617..8639
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8769..8890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8806..8825)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8860..8879
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8939..8955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8939..8955)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8952..8974)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8963..8979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8987..8993,8994..9011,9012..9017))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9032..9053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
     2761 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
     2821 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     2881 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc tcggatccac
     2941 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctcca
     3001 ccatggcccg gggctcggcg ctcccgcggc ggccgctgct gtgcatcccg gccgtctggg
     3061 cggccgccgc gcttctgctc tcagtgtccc ggacttcagg tgaagtggag gttctggatc
     3121 cgaacgaccc tttaggaccc cttgatgggc aggacggccc gattccaact ctgaaaggtt
     3181 actttctgaa ttttctggag ccagtaaaca atatcaccat tgtccaaggc cagacggcaa
     3241 ttctgcactg caaggtggca ggaaacccac cccctaacgt gcggtggcta aagaatgatg
     3301 ccccggtggt gcaggagccg cggcggatca tcatccggaa gacagaatat ggttcacgac
     3361 tgcgaatcca ggacctggac acgacagaca ctggctacta ccagtgcgtg gccaccaacg
     3421 ggatgaagac cattaccgcc actggcgtcc tgtttgtgcg gctgggtcca acgcacagcc
     3481 caaatcataa ctttcaggat gattaccacg aggatgggtt ctgccagcct taccggggaa
     3541 ttgcctgtgc acgcttcatt ggcaaccgga ccatttatgt ggactcgctt cagatgcagg
     3601 gggagattga aaaccgaatc acagcggcct tcaccatgat cggcacgtct acgcacctgt
     3661 cggaccagtg ctcacagttc gccatcccat ccttctgcca cttcgtgttt cctctgtgcg
     3721 acgcgcgctc ccgggcaccc aagccgcgtg agctgtgccg cgacgagtgc gaggtgctgg
     3781 agagcgacct gtgccgccag gagtacacca tcgcccgctc caacccgctc atcctcatgc
     3841 ggcttcagct gcccaagtgt gaggcgctgc ccatgcctga gagccccgac gctgccaact
     3901 gcatgcgcat tggcatccca gccgagaggc tgggccgcta ccatcagtgc tataacggct
     3961 caggcatgga ttacagagga acggcaagca ccaccaagtc aggccaccag tgccagccgt
     4021 gggccctgca gcacccccac agccaccacc tgtccagcac agacttccct gagcttggag
     4081 gggggcacgc ctactgccgg aaccccggag gccagatgga gggcccctgg tgctttacgc
     4141 agaataaaaa cgtacgcatg gaactgtgtg acgtaccctc gtgtagtccc cgagacagca
     4201 gcaagatggg gattctgtac atcttggtcc ccagcatcgc aattccactg gtcatcgctt
     4261 gccttttctt cttggtttgc atgtgccgga ataagcagaa ggcatctgcg tccacaccgc
     4321 agcggcgaca gctgatggcc tcgcccagcc aagacatgga aatgcccctc attaaccagc
     4381 acaaacaggc caaactcaaa gagatcagcc tgtctgcggt gaggttcatg gaggagctgg
     4441 gagaggaccg gtttgggaaa gtctacaaag gtcacctgtt cggccctgcc ccgggggagc
     4501 agacccaggc tgtggccatc aaaacgctga aggacaaagc ggaggggccc ctgcgggagg
     4561 agttccggca tgaggctatg ctgcgagcac ggctgcaaca ccccaacgtc gtctgcctgc
     4621 tgggcgtggt gaccaaggac cagcccctga gcatgatctt cagctactgt tcgcacggcg
     4681 acctccacga attcctggtc atgcgctcgc cgcactcgga cgtgggcagc accgatgatg
     4741 accgcacggt gaagtccgcc ctggagcccc ccgacttcgt gcaccttgtg gcacagatcg
     4801 cggcggggat ggagtaccta tccagccacc acgtggttca caaggacctg gccacccgca
     4861 atgtgctagt gtacgacaag ctgaacgtga agatctcaga cttgggcctc ttccgagagg
     4921 tgtatgccgc cgattactac aagctgctgg ggaactcgct gctgcctatc cgctggatgg
     4981 ccccagaggc catcatgtac ggcaagttct ccatcgactc agacatctgg tcctacggtg
     5041 tggtcctgtg ggaggtcttc agctacggcc tgcagcccta ctgcgggtat tccaaccagg
     5101 atgtggtgga gatgatccgg aaccggcagg tgctgccttg ccccgatgac tgtcccgcct
     5161 gggtgtatgc cctcatgatc gagtgctgga acgagttccc cagccggcgg ccccgcttca
     5221 aggacatcca cagccggctc cgagcctggg gcaacctttc caactacaac agctcggcgc
     5281 agacctcggg ggccagcaac accacgcaga ccagctccct gagcaccagc ccagtgagca
     5341 atgtgagcaa cgcccgctac gtggggccca agcagaaggc cccgcccttc ccacagcccc
     5401 agttcatccc catgaagggc cagatcagac ccatggtgcc cccgccgcag ctctacatcc
     5461 ccgtcaacgg ctaccagccg gtgccggcct atggggccta cctgcccaac ttctacccgg
     5521 tgcagatccc aatgcagatg gccccgcagc aggtgcctcc tcagatggtc cccaagccca
     5581 gctcacacca cagtggcagt ggctccacca gcacaggcta cgtcaccacg gccccctcca
     5641 acacatccat ggcagacagg gcagccctgc tctcagaggg cgctgatgac acacagaacg
     5701 ccccagaaga tggggcccag agcaccgtgc aggaagcaga ggaggaggag gaaggctctg
     5761 tcccagagac tgagctgctg ggggactgtg acactctgca ggtggacgag gcccaagtcc
     5821 agctggaagc tgacccagct ttcttgtaca aagtggttga tatcaaggcc gccgacatca
     5881 ccagcctgta caagaaagtg gggtggagcc atccccagtt cgagaagggc ggaggatctg
     5941 gcggcggaag cggcggagga agctggtccc accctcagtt tgagaaaggc accgagctgg
     6001 gcagcaccat ggccagctac ccctacgacg tgcccgacta cgccaaggac aacaccgtgc
     6061 ccctgaagct gatcgccctg ctggccaacg gcgagttcca ctctggcgag cagctgggcg
     6121 agacactggg catgagcaga gccgccatca acaagcacat ccagaccctg cgggactggg
     6181 gcgtggacgt gttcacagtg cccggcaagg gctacagcct gcccgagccc atccagctgc
     6241 tgaacgccaa gcagatcctg ggccagctgg atggcggatc tgtggccgtg ctgcccgtga
     6301 tcgacagcac caaccagtat ctgctcgacc ggatcggcga gctgaagtcc ggcgacgcct
     6361 gtatcgccga gtaccagcag gccggcagag gcggcagggg cagaaagtgg ttcagcccct
     6421 tcggcgccaa cctgtacctg agcatgttct ggagactgga acagggccca gccgccgcta
     6481 ttggcctgag cctggtcatc ggcatcgtga tggccgaggt gctgcggaag ctgggcgccg
     6541 acaaagtgcg cgtgaagtgg cccaacgacc tgtatctgca ggaccggaag ctggccggca
     6601 tcctggtgga actgaccggc aaaacaggcg acgccgccca gatcgtgatc ggcgctggca
     6661 tcaacatggc catgcggcgg gtggaagaga gcgtggtcaa ccagggctgg atcaccctgc
     6721 aggaagccgg catcaacctg gaccggaaca ccctggccgc catgctgatc agagagctga
     6781 gagccgccct ggaactgttc gagcaggaag gcctggcccc ctacctgagc agatgggaga
     6841 agctggacaa cttcatcaac cggcccgtga aactgatcat cggcgacaaa gagatcttcg
     6901 gcatcagccg gggcatcgac aagcaggggg ccctgctcct ggaacaggac ggcatcatca
     6961 agccctggat gggcggcgag atcagcctgc ggagcgccga gaagtgatga ctcgagtcta
     7021 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7081 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7141 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7201 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7261 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7321 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7381 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7441 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7501 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct
     7561 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa
     7621 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     7681 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     7741 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     7801 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     7861 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     7921 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     7981 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     8041 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     8101 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     8161 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     8221 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     8281 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     8341 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     8401 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     8461 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     8521 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     8581 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     8641 acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8701 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8761 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8821 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8881 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9001 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgct
//
