LOCUS       sequence-372392-        9246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human CSF1R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_CSF1R
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..40)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(274..293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(953..1744,1745..1813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1576..1595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1814..1918)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1993..2012)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2184..2563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2564..2767
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2718..2738
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2769..2787
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2790..2808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2818..2842
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2985..3009
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5932..5956)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6003..6026
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6063..6086
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6117..6143
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6117..6143
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6144..6491,6492..6494,6495..7103)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7147..7164)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7153..7377
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7510..7529)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7661..7708
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7716..8738
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7716..8738
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8868..8989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8905..8924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8959..8978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9038..9054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9038..9054)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9051..9073)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9062..9078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9086..9092,9093..9110,9111..9116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9131..9152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
       61 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1561 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1921 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     1981 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     2041 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     2101 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     2161 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     2221 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2281 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2341 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2401 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2461 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2521 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2581 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2641 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2701 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2761 cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
     2821 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2881 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga
     2941 gctcggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
     3001 aagcaggctc caccatgggc ccaggagttc tgctgctcct gctggtggcc acagcttggc
     3061 atggtcaggg aatcccagtg atagagccca gtgtccctga gctggtcgtg aagccaggag
     3121 caacggtgac cttgcgatgt gtgggcaatg gcagcgtgga atgggatggc cccccatcac
     3181 ctcactggac cctgtactct gatggctcca gcagcatcct cagcaccaac aacgctacct
     3241 tccaaaacac ggggacctat cgctgcactg agcctggaga ccccctggga ggcagcgccg
     3301 ccatccacct ctatgtcaaa gaccctgccc ggccctggaa cgtgctagca caggaggtgg
     3361 tcgtgttcga ggaccaggac gcactactgc cctgtctgct cacagacccg gtgctggaag
     3421 caggcgtctc gctggtgcgt gtgcgtggcc ggcccctcat gcgccacacc aactactcct
     3481 tctcgccctg gcatggcttc accatccaca gggccaagtt cattcagagc caggactatc
     3541 aatgcagtgc cctgatgggt ggcaggaagg tgatgtccat cagcatccgg ctgaaagtgc
     3601 agaaagtcat cccagggccc ccagccttga cactggtgcc tgcagagctg gtgcggattc
     3661 gaggggaggc tgcccagatc gtgtgctcag ccagcagcgt tgatgttaac tttgatgtct
     3721 tcctccaaca caacaacact aagctcgcaa tccctcaaca atctgacttt cataataacc
     3781 gttaccaaaa agtcctgacc ctcaacctcg atcaagtaga tttccaacat gccggcaact
     3841 actcctgcgt ggccagcaac gtgcagggca agcactccac ctccatgttc ttccgggtgg
     3901 tagagagtgc ctacttgaac ttgagctctg agcagaacct catccaggag gtgaccgtgg
     3961 gggaggggct caacctcaaa gtcatggtgg aggcctaccc aggcctgcaa ggttttaact
     4021 ggacctacct gggacccttt tctgaccacc agcctgagcc caagcttgct aatgctacca
     4081 ccaaggacac atacaggcac accttcaccc tctctctgcc ccgcctgaag ccctctgagg
     4141 ctggccgcta ctccttcctg gccagaaacc caggaggctg gagagctctg acgtttgagc
     4201 tcacccttcg atacccccca gaggtaagcg tcatatggac attcatcaac ggctctggca
     4261 cccttttgtg tgctgcctct gggtaccccc agcccaacgt gacatggctg cagtgcagtg
     4321 gccacactga taggtgtgat gaggcccaag tgctgcaggt ctgggatgac ccataccctg
     4381 aggtcctgag ccaggagccc ttccacaagg tgacggtgca gagcctgctg actgttgaga
     4441 ccttagagca caaccaaacc tacgagtgca gggcccacaa cagcgtgggg agtggctcct
     4501 gggccttcat acccatctct gcaggagccc acacgcatcc cccggatgag ttcctcttca
     4561 caccagtggt ggtcgcctgc atgtccatca tggccttgct gctgctgctg ctcctgctgc
     4621 tattgtacaa gtataagcag aagcccaagt accaggtccg ctggaagatc atcgagagct
     4681 atgagggcaa cagttatact ttcatcgacc ccacgcagct gccttacaac gagaagtggg
     4741 agttcccccg gaacaacctg cagtttggta agaccctcgg agctggagcc tttgggaagg
     4801 tggtggaggc cacggccttt ggtctgggca aggaggatgc tgtcctgaag gtggctgtga
     4861 agatgctgaa gtccacggcc catgctgatg agaaggaggc cctcatgtcc gagctgaaga
     4921 tcatgagcca cctgggccag cacgagaaca tcgtcaacct tctgggagcc tgtacccatg
     4981 gaggccctgt actggtcatc acggagtact gttgctatgg cgacctgctc aactttctgc
     5041 gaaggaaggc tgaggccatg ctgggaccca gcctgagccc cggccaggac cccgagggag
     5101 gcgtcgacta taagaacatc cacctcgaga agaaatatgt ccgcagggac agtggcttct
     5161 ccagccaggg tgtggacacc tatgtggaga tgaggcctgt ctccacttct tcaaatgact
     5221 ccttctctga gcaagacctg gacaaggagg atggacggcc cctggagctc cgggacctgc
     5281 ttcacttctc cagccaagta gcccagggca tggccttcct cgcttccaag aattgcatcc
     5341 accgggacgt ggcagcgcgt aacgtgctgt tgaccaatgg tcatgtggcc aagattgggg
     5401 acttcgggct ggctagggac atcatgaatg actccaacta cattgtcaag ggcaatgccc
     5461 gcctgcctgt gaagtggatg gccccagaga gcatctttga ctgtgtctac acggttcaga
     5521 gcgacgtctg gtcctatggc atcctcctct gggagatctt ctcacttggg ctgaatccct
     5581 accctggcat cctggtgaac agcaagttct ataaactggt gaaggatgga taccaaatgg
     5641 cccagcctgc atttgcccca aagaatatat acagcatcat gcaggcctgc tgggccttgg
     5701 agcccaccca cagacccacc ttccagcaga tctgctcctt ccttcaggag caggcccaag
     5761 aggacaggag agagcgggac tataccaatc tgccgagcag cagcagaagc ggtggcagcg
     5821 gcagcagcag cagtgagctg gaggaggaga gctctagtga gcacctgacc tgctgcgagc
     5881 aaggggatat cgcccagccc ttgctgcagc ccaacaacta tcagttctgc gacccagctt
     5941 tcttgtacaa agtggttgat atcaaggccg ccgacatcac cagcctgtac aagaaagtgg
     6001 ggtggagcca tccccagttc gagaagggcg gaggatctgg cggcggaagc ggcggaggaa
     6061 gctggtccca ccctcagttt gagaaaggca ccgagctggg cagcaccatg gccagctacc
     6121 cctacgacgt gcccgactac gccaaggaca acaccgtgcc cctgaagctg atcgccctgc
     6181 tggccaacgg cgagttccac tctggcgagc agctgggcga gacactgggc atgagcagag
     6241 ccgccatcaa caagcacatc cagaccctgc gggactgggg cgtggacgtg ttcacagtgc
     6301 ccggcaaggg ctacagcctg cccgagccca tccagctgct gaacgccaag cagatcctgg
     6361 gccagctgga tggcggatct gtggccgtgc tgcccgtgat cgacagcacc aaccagtatc
     6421 tgctcgaccg gatcggcgag ctgaagtccg gcgacgcctg tatcgccgag taccagcagg
     6481 ccggcagagg cggcaggggc agaaagtggt tcagcccctt cggcgccaac ctgtacctga
     6541 gcatgttctg gagactggaa cagggcccag ccgccgctat tggcctgagc ctggtcatcg
     6601 gcatcgtgat ggccgaggtg ctgcggaagc tgggcgccga caaagtgcgc gtgaagtggc
     6661 ccaacgacct gtatctgcag gaccggaagc tggccggcat cctggtggaa ctgaccggca
     6721 aaacaggcga cgccgcccag atcgtgatcg gcgctggcat caacatggcc atgcggcggg
     6781 tggaagagag cgtggtcaac cagggctgga tcaccctgca ggaagccggc atcaacctgg
     6841 accggaacac cctggccgcc atgctgatca gagagctgag agccgccctg gaactgttcg
     6901 agcaggaagg cctggccccc tacctgagca gatgggagaa gctggacaac ttcatcaacc
     6961 ggcccgtgaa actgatcatc ggcgacaaag agatcttcgg catcagccgg ggcatcgaca
     7021 agcagggggc cctgctcctg gaacaggacg gcatcatcaa gccctggatg ggcggcgaga
     7081 tcagcctgcg gagcgccgag aagtgatgac tcgagtctag agggcccgtt taaacccgct
     7141 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     7201 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     7261 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     7321 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     7381 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     7441 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7501 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7561 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7621 accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt
     7681 cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt
     7741 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     7801 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     7861 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     7921 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     7981 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     8041 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     8101 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     8161 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     8221 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     8281 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     8341 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     8401 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     8461 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     8521 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     8581 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     8641 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     8701 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt
     8761 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     8821 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     8881 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8941 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     9001 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9061 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9241 gtttgc
//
