LOCUS       sequence-372409-        8184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human RYK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_RYK
FEATURES             Location/Qualifiers
     source          1..8184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        140..519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        520..723
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     674..694
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    725..743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    746..764
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     774..798
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    941..965
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2826..2850)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2897..2920
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2957..2980
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3011..3037
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3011..3037
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3038..3385,3386..3388,3389..3997)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4041..4058)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4047..4271
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4404..4423)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4555..4602
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4610..5632
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4610..5632
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5762..5883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5799..5818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5853..5872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5932..5948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5932..5948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5945..5967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5956..5972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5980..5986,5987..6004,6005..6010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6025..6046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6163..6180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6334..6922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6414..6433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7093..7884,7885..7953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7716..7735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7954..8058)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8133..8152)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
       61 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      121 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      361 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      541 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      661 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      721 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
      781 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      841 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc
      901 ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
      961 agaagggccg tcaaggccca ccatgcgtgg ggcggcgcgg ctggggcggc cgggccggag
     1021 ttgcctcccg ggggcccgcg gcctgagggc cccgccgccg ccgccgctgc tgcttctgct
     1081 tgcgctgttg ccgctgctgc ccgcgcctgg cgctgccgcc gcccccgccc cgcggccccc
     1141 ggagctgcag tcggcttccg cggggcccag cgtgagtctc tacctgagcg aggacgaggt
     1201 gcgccggctg atcggtcttg atgcagaact ttattatgtg agaaatgacc ttattagtca
     1261 ctacgctcta tcctttagtc tgttagtacc cagtgagaca aatttcctgc acttcacctg
     1321 gcatgcgaag tccaaggttg aatataagct gggattccaa gtggacaatg ttttggcaat
     1381 ggatatgccc caggtcaaca tttctgttca gggggaagtt ccacgcactt tatcagtgtt
     1441 tcgggtagag ctttcctgta ctggcaaagt agattctgaa gttatgatac taatgcagct
     1501 caacttgaca gtaaattctt caaaaaattt taccgtctta aattttaaac gaaggaaaat
     1561 gtgctacaaa aaacttgaag aagtaaaaac ttcagccttg gacaaaaaca ctagcagaac
     1621 tatttatgat cctgtacatg cagctccaac cacttctacg cgtgtgtttt atattagtgt
     1681 aggggtttgt tgtgcagtaa tatttctcgt agcaataata ttagctgttt tgcaccttca
     1741 tagtatgaaa aggattgaac tggatgacag cattagtgcc agcagtagtt cccaagggct
     1801 gtctcagcca tccacccaga cgactcagta tctgagagca gacacgccca acaatgcaac
     1861 tcctatcacc agctccttag gttatcctac cttgcggata gagaagaacg acttgagaag
     1921 tgtcactctt ttggaggcca aaggcaaggt gaaggatata gcaatatcca gagagaggat
     1981 aactctaaaa gatgtactcc aagaaggtac ttttgggcgt attttccatg ggattttaat
     2041 agatgaaaaa gatccaaata aagaaaaaca agcatttgtc aaaacagtta aagatcaagc
     2101 ttctgaaatt caggtgacaa tgatgctcac tgaaagttgt aagctgcgag gtcttcatca
     2161 cagaaatctt cttcctatta ctcatgtgtg tatagaagaa ggagaaaagc ccatggtgat
     2221 attgccttac atgaattggg ggaatcttaa attgttttta cgacagtgca agttagtaga
     2281 ggccaataat ccacaggcaa tttctcagca agacctggta cacatggcta ttcagattgc
     2341 ctgtggaatg agctacctgg ccagaaggga agtcatccac aaagacctgg ctgccaggaa
     2401 ctgtgtcatt gatgacacac ttcaagttaa gatcacagac aatgccctct ccagagactt
     2461 gttccccatg gactatcact gtctggggga caatgaaaac aggccagttc gttggatggc
     2521 tcttgaaagt ctggttaata acgagttctc tagcgctagt gatgtgtggg cctttggagt
     2581 gacgctgtgg gaactcatga ctctgggcca gactccctac gtggacattg accccttcga
     2641 gatggccgca tacctgaaag atggttaccg aatagcccag ccaatcaact gtcctgatga
     2701 attatttgct gtgatggcct gttgctgggc cttagatcca gaggagaggc ccaagtttca
     2761 gcagctggta cagtgcctaa cagagtttca tgcagccctg ggggcctacg tctcaggcct
     2821 catgggccca gctttcttgt acaaagtggt tgatatcaag gccgccgaca tcaccagcct
     2881 gtacaagaaa gtggggtgga gccatcccca gttcgagaag ggcggaggat ctggcggcgg
     2941 aagcggcgga ggaagctggt cccaccctca gtttgagaaa ggcaccgagc tgggcagcac
     3001 catggccagc tacccctacg acgtgcccga ctacgccaag gacaacaccg tgcccctgaa
     3061 gctgatcgcc ctgctggcca acggcgagtt ccactctggc gagcagctgg gcgagacact
     3121 gggcatgagc agagccgcca tcaacaagca catccagacc ctgcgggact ggggcgtgga
     3181 cgtgttcaca gtgcccggca agggctacag cctgcccgag cccatccagc tgctgaacgc
     3241 caagcagatc ctgggccagc tggatggcgg atctgtggcc gtgctgcccg tgatcgacag
     3301 caccaaccag tatctgctcg accggatcgg cgagctgaag tccggcgacg cctgtatcgc
     3361 cgagtaccag caggccggca gaggcggcag gggcagaaag tggttcagcc ccttcggcgc
     3421 caacctgtac ctgagcatgt tctggagact ggaacagggc ccagccgccg ctattggcct
     3481 gagcctggtc atcggcatcg tgatggccga ggtgctgcgg aagctgggcg ccgacaaagt
     3541 gcgcgtgaag tggcccaacg acctgtatct gcaggaccgg aagctggccg gcatcctggt
     3601 ggaactgacc ggcaaaacag gcgacgccgc ccagatcgtg atcggcgctg gcatcaacat
     3661 ggccatgcgg cgggtggaag agagcgtggt caaccagggc tggatcaccc tgcaggaagc
     3721 cggcatcaac ctggaccgga acaccctggc cgccatgctg atcagagagc tgagagccgc
     3781 cctggaactg ttcgagcagg aaggcctggc cccctacctg agcagatggg agaagctgga
     3841 caacttcatc aaccggcccg tgaaactgat catcggcgac aaagagatct tcggcatcag
     3901 ccggggcatc gacaagcagg gggccctgct cctggaacag gacggcatca tcaagccctg
     3961 gatgggcggc gagatcagcc tgcggagcgc cgagaagtga tgactcgagt ctagagggcc
     4021 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4081 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4141 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4201 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4261 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4321 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4381 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4441 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4501 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta cctagaagtt
     4561 cctattccga agttcctatt ctctagaaag tataggaact tccttggcca aaaagcctga
     4621 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     4681 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     4741 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     4801 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     4861 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     4921 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     4981 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     5041 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     5101 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     5161 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     5221 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     5281 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     5341 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     5401 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     5461 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     5521 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     5581 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtact
     5641 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5701 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5761 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     5941 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6001 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6061 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6121 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6181 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6241 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6301 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6361 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6421 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6481 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6541 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6601 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6661 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6721 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6781 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6841 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6901 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6961 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7021 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7081 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7141 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7201 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7261 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7321 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7381 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7441 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7501 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7561 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7621 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7681 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7741 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7801 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7861 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7921 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7981 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8041 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     8101 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8161 agccagtatc tgctccctgc ttgt
//
