LOCUS       sequence-372424-        4859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage ZL19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_ZL19
FEATURES             Location/Qualifiers
     source          1..4859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     410..429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     663..680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    695..835
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(761..780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     921..943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1099..1977)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2004..2288
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2211..2230
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3611..3638
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3657..3748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3749..3817,3818..4609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3967..3986)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4780..4859,1..509)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
       61 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      121 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      181 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      241 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      301 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      361 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      421 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      481 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      541 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      601 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      661 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      721 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      781 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
      841 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      901 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      961 gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc
     1021 ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca agccgtcaat
     1081 tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca
     1141 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
     1201 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
     1261 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
     1321 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
     1381 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
     1441 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
     1501 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
     1561 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
     1621 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
     1681 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
     1741 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
     1801 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
     1861 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
     1921 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
     1981 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
     2041 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
     2101 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     2161 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     2221 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt
     2281 ttctccatac ccgttttttg ggctaacagg aggaattaac catggtcaag acagacagct
     2341 acgccaatat ctttctcggc ggtagtgatg gtagtgagat ttacgggagc ttgcagaacc
     2401 aggctcctac aattctggct tcgctatatg cggacaatgc tctggtgcgt cgaattatcg
     2461 acacaatccc ggagacagca ttggcggcag gattccatat tgacggcatt gatgacgaac
     2521 ctgcattctg gtcgagatgg gatgatttgg agatgacgca gaatatcaac gatgcctggt
     2581 catgggccag attgttcggt ggggctgcta ttgtcgctat cgtcaaggac aatagagcat
     2641 taacaagccc cgccagggaa ggcgctgagc ttgaaaccgt tcgcgtttac gaccgcactc
     2701 aagtgaaagt tcagactcgc gaggagaatc cgcgcaacgc acgttttggt aagccattaa
     2761 cctatcgcat cacaactaat gagagcgata tgttctatga tgttcattac agccgcattc
     2821 atatcatcga tggcgagcga atccctaacg taatgcgtcg gcaaaatgac ggctggggcc
     2881 gtagcgttct atccagtgac attcttgact caatcaagga ttacaccaac tgtgaacgac
     2941 ttgctacgca attactgaaa cgtaagcagc aagcggtatg gaaagccaaa ggtctggctg
     3001 aactgtgtga tgatagcgaa ggatttggtg cagcaagatt acgcctggcc caggttgaca
     3061 acaatagcgg tgttggtcag gcgattggca ttgacgcaga atctgaagag tacagcgttc
     3121 taaactctga tattggcggc atcgacgcgt ttctggataa gaagttcgat agaatcgttg
     3181 ctctgagcgg cattcatgaa atcatcctga agaacaagaa cgttggaggg gtatcatcaa
     3241 gccagaatac ggcactggag acattccaca agctggttga ccgcaagcgc aatgctgagt
     3301 tgctccctat tcttgagttc ttgatcccgt tcatcgcgaa cgcagaagag tggtcagttg
     3361 agtttaatcc gttggctcag gaatcaagca aggataaggc cgagattctt gagaagaacg
     3421 caaacagcat tgcggcacta attgcggcag gcgctatgga tatcgacgag gctcgtgata
     3481 cactacgaac tatagcccca gaggttaaga tcaatgatgg ttcagtagag acaaaggtga
     3541 caattagtga aaccagtaac gacccgttgg aagtacccac agacaattaa tgccaggcat
     3601 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     3661 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3721 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3781 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3841 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3901 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3961 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     4021 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4081 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4141 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4201 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4261 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4321 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4381 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4441 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4501 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4561 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4621 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4681 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4741 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4801 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgg
//
