LOCUS       sequence-372427-        7145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chimeric Avs4 with transmembrane effector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Avs4_Chimera
FEATURES             Location/Qualifiers
     source          1..7145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5009..5056
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5276..5935)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5850..5869
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5936..6038)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6430..6447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6564..7109)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6644..6663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
       61 ttgtaattct catgttagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
      121 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
      181 cgttgcgcag cgcagctgac aaagattgac cgtgagcgct ctgatggaga aagacgatag
      241 ttgctgagta cgatatcgag ggtacatttc tctgtgtagg ggtagttatt tacaaaaaaa
      301 taggagaata attaaatgaa tgaatttata aaattcctaa agaaccaata taagaacttt
      361 ttcaccaaaa tcgacgacac ctttgataag ttccagaata ccgcaattgg caaattcgtg
      421 atcgagaagc tgatcttacg tgcgaaaagc ttctacagca tcttgtccac cttcggcctc
      481 atcctggttg cgcaaatttc tgcttacaac aacattatgc agtggtccaa caaggtggat
      541 gaacagctga ataacagcga tggttgggcg tatgccggtt ggtttatcgt aagctttatt
      601 gtgccgtctg gtaatatggc agttattgtt gcgctggctc tgatcttgat cgtcgcgagc
      661 attattcgtg acaaagagct gtcgcaaagc aacacgattg agcgcttcat ccactccctg
      721 gaagaaaaga ctaaaaatat tctgtatgaa attcaaacca atatagagtt caaagacaga
      781 agtattgaaa ttgacagacg acattgcata gaacttctac atgagaatct agttcagaaa
      841 aaaattgtca tcgtcagcgg agaaggtggg gttggaaaaa cagcagttat caaaaaaatt
      901 tatgaagcag aaaaacaata cactcctttc tatgtcttta aggctagcga gtttaaaaag
      961 gacagcatta atgagttatt cggtgcgcat ggcttagacg atttctctaa tgctcatcaa
     1021 gacgaattac gtaaagtcat agtcgtagat tctgctgaaa agcttttaga actgaccaat
     1081 atcgatcctt ttaaagaatt cctgactgtt ttaataaagg ataaatggca ggttgttttc
     1141 acaacccgta acaattactt ggcagatctg aactatgctt tcatagatat ttataagata
     1201 actcctggaa acttagtaat aaagaacctt gaacgcggcg agctaataga gttatctgat
     1261 aacaatggat ttagccttcc tcaagatgtt cgattattag aactaatcaa aaatccattt
     1321 tatctaagtg aatatttgag gttctatacc ggtgaaagca tcgattatgt gagcttcaaa
     1381 gaaaagctat ggaataagat tatcgtcaaa aataaacctt ctcgggagca gtgtttctta
     1441 gcgactgctt ttcagcgggc tagtgagggc caattttttg tctccccggc atgtgatact
     1501 ggaattttag atgagttagt taaagacgga attgtcggct atgaagctgc tggttacttc
     1561 attacacatg atatatacga ggaatgggca ttagaaaaga aaatttctgt cgattatatc
     1621 cgtaaagcga acaataacga gttcttcgaa aaaataggag aatcacttcc tgttcgccgt
     1681 agttttcgga attggatatc tgaacgattg cttttagatg accagtccat aaagcctttt
     1741 atcgcagaaa tagtctgtgg agaaggaata tcaaattttt ggaaagacga gttatgggta
     1801 gctgtccttc tttccgacaa ttcaagcata ttttttaatt actttaaaag atatttactt
     1861 agtagtgacc agaatctatt aaaaagactt actttcttat tgaggcttgc ttgcaaggac
     1921 gttgattacg atctgcttaa acagttaggt gtaagtaatt cagatctgct ttccattaaa
     1981 tatgttctta ctaagcctaa gggaactggt tggcagagtg tgatccaatt tatctatgaa
     2041 aatttagatg aaatagggat cagaaatatt aattttatac ttcctgtgat tcaggagtgg
     2101 aatcaaagaa acaaagtggg tgaaacgact cgattatcta gtttgatagc tctaaaatat
     2161 tatcaatgga ctatagatga ggatgtctat ttatccggaa gggataatga gaaaaatatt
     2221 ctgcatacga ttcttcatgg ggcggccatg attaaacctg aaatggaaga ggttttagtt
     2281 aaggttctta aaaataggtg gaaagagcat ggtaccccat atttcgacct tatgacctta
     2341 atccttactg acttagattc atatccggtt tgggcatctc tcccggaata tgttctacaa
     2401 ttggcagatc tgttctggta tcggccactt aaagaaacag gcgaacgtta tcacagtatg
     2461 gatattgaag atgagttcgg tctatttagg tctcatcacg actattatcc agaaagtcca
     2521 tatcagactc ctatatattg gttactacaa tcacagttca aaaaaacaat agactttatt
     2581 cttgatttta cgaacaagac aacgatatgt tttgcccact cccattttgc taaaaacgaa
     2641 attgaagaag tagatgtctt tattgaagaa ggaaagttta taaagcaata tatatgcaat
     2701 cgtctgtggt gctcataccg aggaacacag gtctctacct acttactttc atcaattcat
     2761 atggcattgg aaaagttttt tcttgagaat tttaaaaatg cagactcgaa agtgttggaa
     2821 agttggcttc ttttcttgtt aagaaatacc aagtcagctt ctatttctgc agtagttacg
     2881 agtattgtac ttgcattccc tgagaagaca ttcaatgtag ctaaagtact attccaaaca
     2941 aaggacttct tccgttttga tatgaatcga atggttctag acagaacaca taaaagttca
     3001 ttaatctccc tcagggatgg ctttggcggt acagattaca gaaactcttt gcacgaagaa
     3061 gatagaatta aagcttgcga tgatgtgcat agaaatactt atcttgaaaa tcttgccttg
     3121 cattatcaaa ttttcaggag tgaaaatgta acggagaaag atgccattga aaggcaacaa
     3181 gtgctctggg atattttcga caaatactat aatcagcttc cagatgaagc tcaagaaact
     3241 gaagccgata agacgtggag gctctgcttg gcaagaatgg atcggcgaaa gatgaaaata
     3301 actaccaagg agaaagatga agggattgag atatcattca atcctgagat tgaccctaaa
     3361 ctaaagcaat atagtgagga agcaataaag aaaaactccg agcatatgaa gtatgtaacg
     3421 ctgaaactat gggcaagcta taaaagagaa aaggatgaac gttataagaa ttatggaatg
     3481 tatgaggaca atccgcaaat tgctttacaa gagaccaaag aaataataaa aaagcttaat
     3541 gaggaagggg gtgaagattt cagactatta aatggtaata taccagcaga cgtttgttct
     3601 gtattactgt tagattattt taatcagttg aataatgaag agagagaata ctgtaaagat
     3661 attgttctag cgtattctaa acttccgttg aaggaaggct ataattatca ggtacaagat
     3721 ggaacaacct cggcaatttc agccttaccc gtgatttatc ataattatcc aatggaaagg
     3781 gagactataa aaacaatatt acttttgaca ctgtttaatg accactctat tggaatggca
     3841 ggtgggcgct actcagtatt tcctagtatg gtgattcata aattatggct agactatttt
     3901 gatgatatgc agtccctatt gtttggtttt ttgattttaa agccaaaata tgtaatcctt
     3961 tcaagaaaaa tcattcatga aagttatcgt caagtagact atgacattaa aaaaataaat
     4021 attaataagg tgtttttaaa taactataag cattgcatat caaatgtcat cgataataaa
     4081 atatctatag atgatttggg aagtatggat aaagttgatc tacatatttt gaacacagct
     4141 ttccaattaa ttccagttga tactgttaat attgaacata agaaattggt ttccttaatt
     4201 gttaaaagat tttctacaag cctattgtca agtgttcgag aagatagagt tgattacgct
     4261 cttcggcagt ctttcttgga aagatttgcc tactttacgc ttcatgcgcc cgtgagcgat
     4321 attcccgatt atataaaacc ttttcttgat ggtttcaacg gttcagagcc tatttcagag
     4381 ttatttaaaa aatttattct cgtcgaagat agattaaata cttacgccaa attttggaag
     4441 gtttgggatt tgttttttga taaagtggtt actttgtgca aggatggaga taggtattgg
     4501 tatgtagata aaattataaa aagttacctt tttgctgaat ctccatggaa agaaaactct
     4561 aatggttggc acacatttaa agatagcaat agtcaattct tttgcgatgt atctaggact
     4621 atgggccatt gcccttcaac tttatattct cttgccaaat ctttgaataa cattgccagt
     4681 tgctatctta atcaaggtat aacttggctt tcagaaatat tgtcggttaa taaaaagcta
     4741 tgggaaaaga aattggaaaa tgatactgtt tattatttgg aatgtttggt taggcggtat
     4801 attaacaatg agcgtgagcg aattagacga accaaacagt tgaaacaaga ggtcttagta
     4861 atattggatt ttttggtaga gaaaggatcg gttgttggtt atatgtcacg ggaaaatatt
     4921 ctgtgatgta gttgaaaata ataattttaa tgagagcttt tccaatttag gctccaggga
     4981 ttggagcctt tttattatcg cgcaataact agcataaccc cttggggcct ctaaacgggt
     5041 cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca cactgcttcc
     5101 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc
     5161 tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca
     5221 cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
     5281 ccccgccctg ccactcatcg caatactgtt gtaattcatt taacattctg ccgacatgga
     5341 agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     5401 gcgtataata tttgcccata gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     5461 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     5521 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     5581 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     5641 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     5701 ctttcatcgc catacggaac tctggatgag cattcatcag gcgggcaaga atgtgaataa
     5761 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     5821 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     5881 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     5941 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     6001 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
     6061 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     6121 cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga
     6181 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
     6241 tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc
     6301 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
     6361 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
     6421 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
     6481 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
     6541 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     6601 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6661 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     6721 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     6781 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
     6841 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
     6901 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
     6961 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
     7021 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
     7081 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
     7141 tctag
//
