LOCUS       sequence-372433-        7298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs4 from Escherichia coli NCTC11132.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: EcAvs4
FEATURES             Location/Qualifiers
     source          1..7298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5078..5125
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5345..6004)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5919..5938
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6005..6107)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6499..6516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6633..7178)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6713..6732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagcgca gctgacaaag
      121 attgaccgtg agcgctctga tggagaaaga cgatagttgc tgagtacgat atcgagggta
      181 catttctctg tgtaggggta gttatttaca aaaaaatagg agaataatta aatggtcaaa
      241 ccaaactggg ataactttaa agctaaattt agtgagaatc ctcaaggtaa ttttgagtgg
      301 ttttgctact tgttgttctg tcaagaattc aaaatgcccg caggtatatt tagatataag
      361 aatcaatctg gtatcgaaac taatccaata accaaagata atgaaattat cggttggcaa
      421 tctaaattct atgacacaaa attgtcggat aacaaagctg atcttataga aatgattgag
      481 aaaagcaaaa aggcttatcc aggattaagt aaaatcattt tctatactaa tcaagagtgg
      541 gggcagggga gaaagtccca tgaacctgaa ggcgataaga acgctgataa ttatttggaa
      601 actgtcggaa atagtaacga tcccaaaata aaaattgaag ttgatcagaa agcatatgag
      661 tcgggtatcg aaatagtatg gagagttgct agtttttttg aatcaccgtt tgtaatagtt
      721 gagaatgaaa agattgctaa acatttcttc tcccttaatg aaagcatctt tgatttatta
      781 gaagaaaagc gcaagcacac agaaaatgtt ttatatgaaa ttcaaaccaa tatagagttc
      841 aaagacagaa gtattgaaat tgacagacga cattgcatag aacttctaca tgagaatcta
      901 gttcagaaaa aaattgtcat cgtcagcgga gaaggtgggg ttggaaaaac agcagttatc
      961 aaaaaaattt atgaagcaga aaaacaatac actcctttct atgtctttaa ggctagcgag
     1021 tttaaaaagg acagcattaa tgagttattc ggtgcgcatg gcttagacga tttctctaat
     1081 gctcatcaag acgaattacg taaagtcata gtcgtagatt ctgctgaaaa gcttttagaa
     1141 ctgaccaata tcgatccttt taaagaattc ctgactgttt taataaagga taaatggcag
     1201 gttgttttca caacccgtaa caattacttg gcagatctga actatgcttt catagatatt
     1261 tataagataa ctcctggaaa cttagtaata aagaaccttg aacgcggcga gctaatagag
     1321 ttatctgata acaatggatt tagccttcct caagatgttc gattattaga actaatcaaa
     1381 aatccatttt atctaagtga atatttgagg ttctataccg gtgaaagcat cgattatgtg
     1441 agcttcaaag aaaagctatg gaataagatt atcgtcaaaa ataaaccttc tcgggagcag
     1501 tgtttcttag cgactgcttt tcagcgggct agtgagggcc aattttttgt ctccccggca
     1561 tgtgatactg gaattttaga tgagttagtt aaagacggaa ttgtcggcta tgaagctgct
     1621 ggttacttca ttacacatga tatatacgag gaatgggcat tagaaaagaa aatttctgtc
     1681 gattatatcc gtaaagcgaa caataacgag ttcttcgaaa aaataggaga atcacttcct
     1741 gttcgccgta gttttcggaa ttggatatct gaacgattgc ttttagatga ccagtccata
     1801 aagcctttta tcgcagaaat agtctgtgga gaaggaatat caaatttttg gaaagacgag
     1861 ttatgggtag ctgtccttct ttccgacaat tcaagcatat tttttaatta ctttaaaaga
     1921 tatttactta gtagtgacca gaatctatta aaaagactta ctttcttatt gaggcttgct
     1981 tgcaaggacg ttgattacga tctgcttaaa cagttaggtg taagtaattc agatctgctt
     2041 tccattaaat atgttcttac taagcctaag ggaactggtt ggcagagtgt gatccaattt
     2101 atctatgaaa atttagatga aatagggatc agaaatatta attttatact tcctgtgatt
     2161 caggagtgga atcaaagaaa caaagtgggt gaaacgactc gattatctag tttgatagct
     2221 ctaaaatatt atcaatggac tatagatgag gatgtctatt tatccggaag ggataatgag
     2281 aaaaatattc tgcatacgat tcttcatggg gcggccatga ttaaacctga aatggaagag
     2341 gttttagtta aggttcttaa aaataggtgg aaagagcatg gtaccccata tttcgacctt
     2401 atgaccttaa tccttactga cttagattca tatccggttt gggcatctct cccggaatat
     2461 gttctacaat tggcagatct gttctggtat cggccactta aagaaacagg cgaacgttat
     2521 cacagtatgg atattgaaga tgagttcggt ctatttaggt ctcatcacga ctattatcca
     2581 gaaagtccat atcagactcc tatatattgg ttactacaat cacagttcaa aaaaacaata
     2641 gactttattc ttgattttac gaacaagaca acgatatgtt ttgcccactc ccattttgct
     2701 aaaaacgaaa ttgaagaagt agatgtcttt attgaagaag gaaagtttat aaagcaatat
     2761 atatgcaatc gtctgtggtg ctcataccga ggaacacagg tctctaccta cttactttca
     2821 tcaattcata tggcattgga aaagtttttt cttgagaatt ttaaaaatgc agactcgaaa
     2881 gtgttggaaa gttggcttct tttcttgtta agaaatacca agtcagcttc tatttctgca
     2941 gtagttacga gtattgtact tgcattccct gagaagacat tcaatgtagc taaagtacta
     3001 ttccaaacaa aggacttctt ccgttttgat atgaatcgaa tggttctaga cagaacacat
     3061 aaaagttcat taatctccct cagggatggc tttggcggta cagattacag aaactctttg
     3121 cacgaagaag atagaattaa agcttgcgat gatgtgcata gaaatactta tcttgaaaat
     3181 cttgccttgc attatcaaat tttcaggagt gaaaatgtaa cggagaaaga tgccattgaa
     3241 aggcaacaag tgctctggga tattttcgac aaatactata atcagcttcc agatgaagct
     3301 caagaaactg aagccgataa gacgtggagg ctctgcttgg caagaatgga tcggcgaaag
     3361 atgaaaataa ctaccaagga gaaagatgaa gggattgaga tatcattcaa tcctgagatt
     3421 gaccctaaac taaagcaata tagtgaggaa gcaataaaga aaaactccga gcatatgaag
     3481 tatgtaacgc tgaaactatg ggcaagctat aaaagagaaa aggatgaacg ttataagaat
     3541 tatggaatgt atgaggacaa tccgcaaatt gctttacaag agaccaaaga aataataaaa
     3601 aagcttaatg aggaaggggg tgaagatttc agactattaa atggtaatat accagcagac
     3661 gtttgttctg tattactgtt agattatttt aatcagttga ataatgaaga gagagaatac
     3721 tgtaaagata ttgttctagc gtattctaaa cttccgttga aggaaggcta taattatcag
     3781 gtacaagatg gaacaacctc ggcaatttca gccttacccg tgatttatca taattatcca
     3841 atggaaaggg agactataaa aacaatatta cttttgacac tgtttaatga ccactctatt
     3901 ggaatggcag gtgggcgcta ctcagtattt cctagtatgg tgattcataa attatggcta
     3961 gactattttg atgatatgca gtccctattg tttggttttt tgattttaaa gccaaaatat
     4021 gtaatccttt caagaaaaat cattcatgaa agttatcgtc aagtagacta tgacattaaa
     4081 aaaataaata ttaataaggt gtttttaaat aactataagc attgcatatc aaatgtcatc
     4141 gataataaaa tatctataga tgatttggga agtatggata aagttgatct acatattttg
     4201 aacacagctt tccaattaat tccagttgat actgttaata ttgaacataa gaaattggtt
     4261 tccttaattg ttaaaagatt ttctacaagc ctattgtcaa gtgttcgaga agatagagtt
     4321 gattacgctc ttcggcagtc tttcttggaa agatttgcct actttacgct tcatgcgccc
     4381 gtgagcgata ttcccgatta tataaaacct tttcttgatg gtttcaacgg ttcagagcct
     4441 atttcagagt tatttaaaaa atttattctc gtcgaagata gattaaatac ttacgccaaa
     4501 ttttggaagg tttgggattt gttttttgat aaagtggtta ctttgtgcaa ggatggagat
     4561 aggtattggt atgtagataa aattataaaa agttaccttt ttgctgaatc tccatggaaa
     4621 gaaaactcta atggttggca cacatttaaa gatagcaata gtcaattctt ttgcgatgta
     4681 tctaggacta tgggccattg cccttcaact ttatattctc ttgccaaatc tttgaataac
     4741 attgccagtt gctatcttaa tcaaggtata acttggcttt cagaaatatt gtcggttaat
     4801 aaaaagctat gggaaaagaa attggaaaat gatactgttt attatttgga atgtttggtt
     4861 aggcggtata ttaacaatga gcgtgagcga attagacgaa ccaaacagtt gaaacaagag
     4921 gtcttagtaa tattggattt tttggtagag aaaggatcgg ttgttggtta tatgtcacgg
     4981 gaaaatattc tgtgatgtag ttgaaaataa taattttaat gagagctttt ccaatttagg
     5041 ctccagggat tggagccttt ttattatcgc gcaataacta gcataacccc ttggggcctc
     5101 taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac
     5161 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
     5221 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
     5281 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa
     5341 aaaattacgc cccgccctgc cactcatcgc aatactgttg taattcattt aacattctgc
     5401 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
     5461 tgtcgccttg cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat
     5521 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     5581 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     5641 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     5701 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     5761 gctcaccgtc tttcatcgcc atacggaact ctggatgagc attcatcagg cgggcaagaa
     5821 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     5881 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     5941 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     6001 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     6061 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     6121 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg
     6181 cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca
     6241 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
     6301 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
     6361 catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
     6421 aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
     6481 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     6541 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
     6601 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
     6661 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     6721 caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     6781 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     6841 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     6901 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
     6961 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
     7021 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
     7081 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
     7141 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga
     7201 taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc
     7261 ccatacgata taagttgtaa ttctcatgtt agtcatgc
//
