LOCUS       sequence-37245-e        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-G-GECO1.1
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..60
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(64..72,73..87)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             115..171
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     384..405
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(772..791)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             907..1350
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
       61 cgggatctgt acgacgatga tgataaggat ctcgccacaa tggtcgactc atcacgtcgt
      121 aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagctc actcgagaac
      181 gtctatatca aggccgacga gcagaagaac ggcatcaagg cgtacttcaa gatccgccac
      241 aacatcgagg gcggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
      301 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccat actttcgaaa
      361 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      421 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc
      481 gaggagctgt tcaccggggt ggtgcccatc caggtcgagc tggacggcga cgtaaacggc
      541 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg
      601 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      661 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      721 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
      781 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      841 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      901 acgcgtgacc aactgactga agagcagatc gcagaattta aagaggcttt ctccctattt
      961 gacaaggacg gggatgggac gataacaacc aaggagctgg ggacggtgat gcggtctctg
     1021 gggcagaacc ccacagaagc agagctgcag gacatgatca atgaagtaga tgccgacggt
     1081 gacggcacaa tcgacttccc tgagttcctg acgatgatgg caagaaaaat gaatgacaca
     1141 gacagtgaag aggaaattag agaagcgttc cgtgtgtttg ataaggacgg caatggctac
     1201 atcggcgcag cagagcttcg ccacgtgatg acaaaccttg gagagaagtt aacagatgaa
     1261 gaggttgatg aaatgatcag ggtagcagac atcgatgggg atggtcaggt aaactacgaa
     1321 gagtttgtac aaatgatgac agcgaagtag
//
