LOCUS       sequence-37247-e        1356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: pTorPE-G-GECO1.2
FEATURES             Location/Qualifiers
     source          1..1356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..60
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(64..72,73..87)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             115..171
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     384..405
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             913..1356
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
       61 cgggatctgt acgacgatga tgataaggat ctcgccacaa tggtcgactc atcacgtcgt
      121 aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagctc actcgagaac
      181 gtctatatca aggccgacga gcagaagaac ggcatcaagg cgtacttcaa gatccgccac
      241 aacatcgagg gcggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
      301 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccat gctttcgaaa
      361 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      421 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcgaatc catggtgagc
      481 aagggcgagg agctgttcac cggggtggtg cccatccagg tcgagctgga cggcgacgta
      541 aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta cggcaagctg
      601 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      661 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      721 ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt cttcaagggc
      781 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      841 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      901 tacaacacgc gtgaccaact gactgaagag cagatcgcag aatttaaaga ggctttctcc
      961 ctatttgaca aggacgggga tgggacgata acaaccaagg agctggggac ggtgatgcgg
     1021 tctctggggc agaaccccac agaagcagag ctgcaggaca tgatcaatga agtagatgcc
     1081 gacggtgacg gcacaatcga cttccctgag ttcctgacaa tgatggcaag aaaaatgaaa
     1141 gacacagaca gtgaagaaga aattagagaa gcgttccgtg tgtttgataa ggacggcaat
     1201 ggctacatcg gcgcagcaga gcttcgccac gtgatgacaa accttggaga gaagttaaca
     1261 gatgaagagg ttgatgaaat gatcagggta gcagacatcg atggggatgg tcaggtaaac
     1321 tacgaagagt ttgtacaaat gatgacagcg aagtag
//
