LOCUS       sequence-372472-        8402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 
            around GFP for subcloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, 
            Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella 
            BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, 
            Ianari A
  TITLE     Systematic profiling of conditional degron tag technologies for 
            target validation studies.
  JOURNAL   Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4.
  PUBMED    36127368
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-20"; volume: "13(1):5495. doi"; pages: " 
            10.1038/s41467-022-33246-4"
COMMENT     Sequence Label: PGK-AID-NTERM-GFP
FEATURES             Location/Qualifiers
     source          1..8402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     239..259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        241..259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        287..513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             514..694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    741..866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1359..1592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1777..1821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2012..2512
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2431..2449
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3194..3250
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3251..3292
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3293..3496
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             join(3554..3556,3557..4267)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    4345..4462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4541..5129
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4594..4614)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5012..5023)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5201..5434
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5512..5633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5549..5568)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5603..5622
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5673..5808
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5828..5847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5829..5847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5857..5874)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5857..5873)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5866..5888)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6015..6470
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6102..6121)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6312..6333
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6496..6600
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6601..6669,6670..7461)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6819..6838)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7632..8220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8121..8140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8374..8391
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1201 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1261 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1321 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1381 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1441 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1501 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1561 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1621 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1681 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1741 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1801 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1861 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1921 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1981 accccgaggg gaccctattc cagcacatat gttgcgcctt ttccaaggca gccctgggtt
     2041 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     2101 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     2161 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     2221 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     2281 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     2341 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     2401 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     2461 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcagactga
     2521 gtcggccggt cgaatcaagc ttatcgatac cgtcgactcc ggaatagcca ccatagccac
     2581 cgccaccatg accgagtaca agccaactgt ccgcttggca acccgcgacg atgtgcccag
     2641 ggccgtccgc acgctggctg ctgccttcgc cgactaccca gccacccgcc acaccgtgga
     2701 cccggaccgc cacatcgagc gcgtcaccga gctgcaggag ctcttcctca cccgagttgg
     2761 ccttgacatt ggcaaggtct gggtggcgga cgacggtgct gccgtggccg tctggaccac
     2821 ccccgagtcg gtcgaggctg gcgccgtgtt cgccgagatc gggccgcgca tggccgagct
     2881 ttcgggctcc cggttggctg cccaacagca gatggagggg ctgttggcac cccaccgccc
     2941 caaggagccg gcctggttcc tggccacagt tggtgtctct cccgaccacc agggcaaggg
     3001 cctgggcagc gccgtggtgc tgcctggggt cgaggctgcc gagcgcgctg gcgtccctgc
     3061 cttcctggag acctctgccc ctcgcaactt gcccttctac gagcgcctgg gcttcaccgt
     3121 caccgcggac gtcgaggtgc ccgaggggcc gcgcacctgg tgcatgaccc gcaagcctgg
     3181 ggcaggctcc ggagccacca acttttccct gctgaagcag gctggcgacg tcgaggagaa
     3241 ccctggccct ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgaaggagaa
     3301 gagtgcttgt cctaaagatc cagccaaacc tccggccaag gcacaagttg tgggatggcc
     3361 accggtgaga tcataccgga agaacgtgat ggtttcctgc caaaaatcaa gcggtggccc
     3421 ggaggcggcg gcgttcgtga aggtatcaat ggacggagca ccgtacttga ggaaaatcga
     3481 tttgaggatg tataaagctg aagctgctgc aaaggaagct gcagctaagg aggctgcagc
     3541 taaggctgga tccgtcagta aaggggaaga attgtttacg ggtgttgttc cgatacttgt
     3601 tgaattggat ggggatgtga atggtcataa attttccgtt agtggtgaag gggaaggtga
     3661 cgctacttat ggtaaattga cattgaaatt tatttgtact acaggaaaac ttccagtccc
     3721 atggccgact ttggtaacga ctcttacata tggagtccaa tgttttagta ggtatccaga
     3781 tcacatgaaa caacatgatt tctttaaatc agcaatgcct gagggatatg ttcaagaacg
     3841 aacaatattc tttaaagatg atgggaacta taagacaagg gcagaagtca aatttgaagg
     3901 tgatacactc gttaatcgga ttgaactgaa agggatagat tttaaggaag atgggaatat
     3961 tcttgggcac aaacttgaat ataattataa ttcccataat gtatacataa tggctgacaa
     4021 gcagaagaat ggaattaaag tcaattttaa aataaggcat aatatagaag atgggagtgt
     4081 ccaattggct gatcattacc aacaaaatac acctattggg gatggtcctg tcctcttgcc
     4141 tgataatcat tatctttcta cacaatctgc tctctcaaag gaccctaatg aaaagcgcga
     4201 ccatatggtg ttgttggaat ttgtcactgc tgcaggcatt accctgggaa tggatgaact
     4261 ctataaatga gaattctagg tacaagtaaa ctagtaagct tggcgtaact agatcttgag
     4321 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     4381 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     4441 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttgggc
     4501 tcgagcttgg ttcattctca agcctacgcg ttaagtcgac aatcaacctc tggattacaa
     4561 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     4621 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     4681 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     4741 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4801 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4861 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4921 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     4981 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5041 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5101 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     5161 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5221 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5281 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5341 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5401 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     5461 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     5521 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5581 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5641 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5701 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5761 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     5821 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     5881 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5941 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6001 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6061 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6121 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6181 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6241 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6301 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6361 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6421 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     6481 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6541 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6601 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6661 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6721 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6781 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6841 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6901 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6961 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7021 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7081 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7141 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7201 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7261 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7321 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7381 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7441 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7501 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7741 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7921 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7981 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8041 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8101 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8161 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8221 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8281 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8341 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8401 ga
//
