LOCUS       sequence-372497-        8676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human MUSK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 8676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_MUSK
FEATURES             Location/Qualifiers
     source          1..8676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        125..504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        505..708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     659..679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    710..728
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    731..749
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     759..783
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    926..950
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3303..3327)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3374..3397
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3434..3457
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3488..3514
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3488..3514
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3515..3862,3863..3865,3866..4474)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4518..4535)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4524..4748
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4881..4900)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5032..5079
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5087..6109
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5087..6109
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6239..6360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6276..6295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6330..6349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6409..6425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6409..6425)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6422..6444)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6433..6449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6457..6463,6464..6481,6482..6487))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6502..6523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6640..6657)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6811..7399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6891..6910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7570..8361,8362..8430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8193..8212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8431..8535)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8610..8629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
       61 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
      121 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      301 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt
      721 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
      781 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      841 agcctccgga ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca
      901 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagttggca ccatgagaga
      961 gctcgtcaac attccactgg tacatattct tactctggtt gccttcagcg gaactgagaa
     1021 acttccaaaa gctcctgtca tcaccactcc tcttgaaaca gtggatgcct tagttgaaga
     1081 agtggctact ttcatgtgtg cagtggaatc ctacccccag cctgagattt cctggactag
     1141 aaataaaatt ctcattaaac tctttgacac ccggtacagc atccgggaga atgggcagct
     1201 cctcaccatc ctgagtgtgg aagacagtga tgatggcatt tactgctgca cggccaacaa
     1261 tggtgtggga ggagctgtgg agagttgtgg agccctgcaa gtgaagatga aacctaaaat
     1321 aactcgtcct cccataaatg tgaaaataat agaaggatta aaagcagtcc taccatgtac
     1381 tacaatgggt aatcccaaac catcagtgtc ttggataaag ggagacagcc ctctcaggga
     1441 aaattcccga attgcagttc ttgaatctgg gagcttgagg attcataacg tacaaaagga
     1501 agatgcagga cagtatcgat gtgtggcaaa aaacagcctc gggacagcat attccaaagt
     1561 ggtgaagctg gaagttgagg aagaaagtga acccgaacaa gatactaaag tttttgccag
     1621 gatcctgcgg gctcctgaat cccacaatgt cacctttggc tcctttgtga ccctgcactg
     1681 tacagcaaca ggcattcctg tccccaccat cacctggatt gaaaacggaa atgctgtttc
     1741 ttctgggtcc attcaagaga gtgtgaaaga ccgagtgatt gactcaagac tgcagctgtt
     1801 tatcaccaag ccaggactct acacatgcat agctaccaat aagcatgggg agaagttcag
     1861 tactgccaag gctgcagcca ccatcagcat agcagaatgg agagagtact gcttggcagt
     1921 aaaggagctc ttctgcgcaa aagaatggct ggtaatggaa gagaagaccc acagaggact
     1981 ctacagatcc gagatgcatt tgctgtccgt gccagaatgc agcaagcttc ccagcatgca
     2041 ttgggacccc acggcctgtg ccagactgcc acatctagca ttcccaccaa tgacgtcctc
     2101 aaagccaagt gtggacattc caaatctgcc ttcctcctcc tcttcttcct tctctgtctc
     2161 acctacatac tccatgactg taataatctc catcatgtcc agctttgcaa tatttgtgct
     2221 tcttaccata actactctct attgctgccg aagaagaaaa caatggaaaa ataagaaaag
     2281 agaatcagca gcagtaaccc tcaccacact gccttctgag ctcttactag atagacttca
     2341 tcccaacccc atgtaccaga ggatgccgct ccttctgaac cccaaattgc tcagcctgga
     2401 gtatccaagg aataacattg aatatgtgag agacatcgga gagggagcgt ttggaagggt
     2461 gtttcaagca agggcaccag gcttacttcc ctatgaacct ttcactatgg tggcagtaaa
     2521 gatgctcaaa gaagaagcct cggcagatat gcaagcggac tttcagaggg aggcagccct
     2581 catggcagaa tttgacaacc ctaacattgt gaagctatta ggagtgtgtg ctgtcgggaa
     2641 gccaatgtgc ctgctctttg aatacatggc ctatggtgac ctcaatgagt tcctccgcag
     2701 catgtcccct cacaccgtgt gcagcctcag tcacagtgac ttgtctatga gggctcaggt
     2761 ctccagccct gggcccccac ccctctcctg tgctgagcag ctttgcattg ccaggcaggt
     2821 ggcagctggc atggcttacc tctcagaacg taagtttgtt caccgagatt tagccaccag
     2881 gaactgcctg gtgggcgaga acatggtggt gaaaattgcc gactttggcc tctccaggaa
     2941 catctactca gcagactact acaaagctaa tgaaaacgac gctatcccta tccgttggat
     3001 gccaccagag tccatttttt ataaccgcta cactacagag tctgatgtgt gggcctatgg
     3061 cgtggtcctc tgggagatct tctcctatgg cctgcagccc tactatggga tggcccatga
     3121 ggaggtcatt tactacgtgc gagatggcaa catcctctcc tgccctgaga actgccccgt
     3181 ggagctgtac aatctcatgc gtctatgttg gagcaagctg cctgcagaca gacccagttt
     3241 caccagtatt caccgaattc tggaacgcat gtgtgagagg gcagagggaa ctgtgagtgt
     3301 cttgccaact ttcttgtaca aagtggttga tatcaaggcc gccgacatca ccagcctgta
     3361 caagaaagtg gggtggagcc atccccagtt cgagaagggc ggaggatctg gcggcggaag
     3421 cggcggagga agctggtccc accctcagtt tgagaaaggc accgagctgg gcagcaccat
     3481 ggccagctac ccctacgacg tgcccgacta cgccaaggac aacaccgtgc ccctgaagct
     3541 gatcgccctg ctggccaacg gcgagttcca ctctggcgag cagctgggcg agacactggg
     3601 catgagcaga gccgccatca acaagcacat ccagaccctg cgggactggg gcgtggacgt
     3661 gttcacagtg cccggcaagg gctacagcct gcccgagccc atccagctgc tgaacgccaa
     3721 gcagatcctg ggccagctgg atggcggatc tgtggccgtg ctgcccgtga tcgacagcac
     3781 caaccagtat ctgctcgacc ggatcggcga gctgaagtcc ggcgacgcct gtatcgccga
     3841 gtaccagcag gccggcagag gcggcagggg cagaaagtgg ttcagcccct tcggcgccaa
     3901 cctgtacctg agcatgttct ggagactgga acagggccca gccgccgcta ttggcctgag
     3961 cctggtcatc ggcatcgtga tggccgaggt gctgcggaag ctgggcgccg acaaagtgcg
     4021 cgtgaagtgg cccaacgacc tgtatctgca ggaccggaag ctggccggca tcctggtgga
     4081 actgaccggc aaaacaggcg acgccgccca gatcgtgatc ggcgctggca tcaacatggc
     4141 catgcggcgg gtggaagaga gcgtggtcaa ccagggctgg atcaccctgc aggaagccgg
     4201 catcaacctg gaccggaaca ccctggccgc catgctgatc agagagctga gagccgccct
     4261 ggaactgttc gagcaggaag gcctggcccc ctacctgagc agatgggaga agctggacaa
     4321 cttcatcaac cggcccgtga aactgatcat cggcgacaaa gagatcttcg gcatcagccg
     4381 gggcatcgac aagcaggggg ccctgctcct ggaacaggac ggcatcatca agccctggat
     4441 gggcggcgag atcagcctgc ggagcgccga gaagtgatga ctcgagtcta gagggcccgt
     4501 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4561 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4621 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4681 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4741 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4801 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4861 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4921 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4981 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct
     5041 attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact
     5101 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     5161 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     5221 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     5281 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     5341 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     5401 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     5461 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     5521 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     5581 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     5641 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     5701 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     5761 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     5821 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     5881 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     5941 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     6001 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     6061 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg
     6121 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6181 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     6241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6301 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6361 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     6421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     8581 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8641 cagtatctgc tccctgcttg tgtgttggag gtcgct
//
