LOCUS       sequence-372504-        8425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 
            around GFP for subcloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, 
            Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella 
            BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, 
            Ianari A
  TITLE     Systematic profiling of conditional degron tag technologies for 
            target validation studies.
  JOURNAL   Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4.
  PUBMED    36127368
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-20"; volume: "13(1):5495. doi"; pages: " 
            10.1038/s41467-022-33246-4"
COMMENT     Sequence Label: SFFV-DTAG-NTERM-GFP
FEATURES             Location/Qualifiers
     source          1..8425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     239..259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        241..259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        287..513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             514..694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    741..866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1359..1592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1777..1821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2023..2429
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3094..3150
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3151..3192
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3193..3513
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(3577..3579,3580..4290)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    4368..4485
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4564..5152
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4617..4637)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5035..5046)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5224..5457
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5535..5656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5572..5591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5626..5645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5696..5831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5851..5870)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5852..5870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5880..5897)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5880..5896)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5889..5911)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6038..6493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6125..6144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6335..6356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6519..6623
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6624..6692,6693..7484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6842..6861)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7655..8243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8144..8163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8397..8414
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1201 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1261 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1321 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1381 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1441 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1501 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1561 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1621 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1681 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1741 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1801 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1861 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1921 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1981 accccgaggg gaccctattc cagcacatat gagctagctg cagtaacgcc attttgcaag
     2041 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
     2101 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
     2161 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
     2221 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
     2281 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
     2341 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
     2401 ccagtcctcc gacagactga gtcggccggt cgaatcaagc ttatcgatac cgtcgactcc
     2461 ggaatagcca ccatagccac cgccaccatg accgagtaca agccaactgt ccgcttggca
     2521 acccgcgacg atgtgcccag ggccgtccgc acgctggctg ctgccttcgc cgactaccca
     2581 gccacccgcc acaccgtgga cccggaccgc cacatcgagc gcgtcaccga gctgcaggag
     2641 ctcttcctca cccgagttgg ccttgacatt ggcaaggtct gggtggcgga cgacggtgct
     2701 gccgtggccg tctggaccac ccccgagtcg gtcgaggctg gcgccgtgtt cgccgagatc
     2761 gggccgcgca tggccgagct ttcgggctcc cggttggctg cccaacagca gatggagggg
     2821 ctgttggcac cccaccgccc caaggagccg gcctggttcc tggccacagt tggtgtctct
     2881 cccgaccacc agggcaaggg cctgggcagc gccgtggtgc tgcctggggt cgaggctgcc
     2941 gagcgcgctg gcgtccctgc cttcctggag acctctgccc ctcgcaactt gcccttctac
     3001 gagcgcctgg gcttcaccgt caccgcggac gtcgaggtgc ccgaggggcc gcgcacctgg
     3061 tgcatgaccc gcaagcctgg ggcaggctcc ggagccacca acttttccct gctgaagcag
     3121 gctggcgacg tcgaggagaa ccctggccct ggtaagccta tccctaaccc tctcctcggt
     3181 ctcgattcta cgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc
     3241 aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt
     3301 gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc
     3361 cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata
     3421 tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact
     3481 ctcgtcttcg atgtggagct tctaaaactg gaaacgcgtg ctgaagctgc tgcaaaggaa
     3541 gctgcagcta aggaggctgc agctaaggct ggatccgtca gtaaagggga agaattgttt
     3601 acgggtgttg ttccgatact tgttgaattg gatggggatg tgaatggtca taaattttcc
     3661 gttagtggtg aaggggaagg tgacgctact tatggtaaat tgacattgaa atttatttgt
     3721 actacaggaa aacttccagt cccatggccg actttggtaa cgactcttac atatggagtc
     3781 caatgtttta gtaggtatcc agatcacatg aaacaacatg atttctttaa atcagcaatg
     3841 cctgagggat atgttcaaga acgaacaata ttctttaaag atgatgggaa ctataagaca
     3901 agggcagaag tcaaatttga aggtgataca ctcgttaatc ggattgaact gaaagggata
     3961 gattttaagg aagatgggaa tattcttggg cacaaacttg aatataatta taattcccat
     4021 aatgtataca taatggctga caagcagaag aatggaatta aagtcaattt taaaataagg
     4081 cataatatag aagatgggag tgtccaattg gctgatcatt accaacaaaa tacacctatt
     4141 ggggatggtc ctgtcctctt gcctgataat cattatcttt ctacacaatc tgctctctca
     4201 aaggacccta atgaaaagcg cgaccatatg gtgttgttgg aatttgtcac tgctgcaggc
     4261 attaccctgg gaatggatga actctataaa tgagaattct aggtacaagt aaactagtaa
     4321 gcttggcgta actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     4381 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4441 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     4501 gacagcagag atccactttg ggctcgagct tggttcattc tcaagcctac gcgttaagtc
     4561 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4621 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4681 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4741 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4801 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4861 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4921 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4981 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5041 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5101 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     5161 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5221 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5281 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5341 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5401 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5461 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5521 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5581 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5641 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     5701 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5761 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5821 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     5881 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5941 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6001 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6061 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6121 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6181 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6241 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6301 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6361 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6421 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6481 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6541 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6601 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6661 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6721 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6781 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6841 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6901 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6961 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7021 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7081 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7141 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7201 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7261 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7321 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7381 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7441 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7501 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7561 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7801 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8281 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8341 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8401 agtcagtgag cgaggaagcg gaaga
//
