LOCUS       sequence-37256-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter construct for human ACE2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Senkel S, Lucas B, Klein-Hitpass L, Ryffel GU
  TITLE     Identification of target genes of the transcription factor HNF1beta 
            and HNF1alpha in a human embryonic kidney cell line.
  JOURNAL   Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.
  PUBMED    16297991
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2005 Dec 20. 1731(3):179-90."
COMMENT     Sequence Label: ACE2(-252)-luc
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    491..582
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(523..542)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    596..644
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctcgggaga atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta
       61 ccaacagtac cggaatgcca agcttactta gatcgcagat ctcgtcccct gtgagccaag
      121 atcacatcca ctgaatgact ttccctagac taaaacctcc tcatgagatt ttctctctta
      181 tcagcctttg aacttgggtt gggcgctgag caggaaagac caaaaaaaga aaaagaagga
      241 gaacacagta aacaatctgc tgagccaata taaagttcat cctggagagg acagatatgt
      301 aacagatttt agaataattt tttaaagtga atcaaataag aatacgttat tctttaatcc
      361 tagagaacct tacctctcct gtcactttat ggccaaatca aaaccggtac ttttggttaa
      421 tattttccta gaaagctgtt aaagatacac ggaaaattct agcacgcgta agagctcggt
      481 acctatcgat agagaaatgt tctggcacct gcacttgcac tggggacagc ctattttgct
      541 agtttgtttt gtttcgtttt gttttgatgg agagcgtatg ttagtactat cgattcacac
      601 aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc gctccaagta
      661 cctcccgtac cttaatatta cttacttatc atggtagctt gggctggcgt aatagcgaag
      721 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg
      781 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
      841 accaataggc cgaaatcggc aaaatcccct tataaatcaa aagaatagac cgagataggg
//
