LOCUS       sequence-37257-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter construct for human ACE2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Senkel S, Lucas B, Klein-Hitpass L, Ryffel GU
  TITLE     Identification of target genes of the transcription factor HNF1beta 
            and HNF1alpha in a human embryonic kidney cell line.
  JOURNAL   Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.
  PUBMED    16297991
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2005 Dec 20. 1731(3):179-90."
COMMENT     Sequence Label: ACE2(-202)-luc
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    436..527
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(468..487)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    541..589
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggnngatggc gccgggcctt tctttatgtt tttggcgtct tccatggtgg ctttaccaac
       61 agtaccggaa tgccaagctt acttagatcg cagatctcgt cccctgtgag ccaagatcac
      121 atccactgaa tgactttccc tagactaaaa cctcctcatg agattttctc tcttatcagc
      181 ctttgaactt gggttgggcg ctgagcagga aagaccaaaa aaagaaaaag aaggagaaca
      241 cagtaaacaa tctgctgagc caatataaag ttcatcctgg agaggacaga tatgtaacag
      301 attttagaat aattttttaa agtgaatcaa ataagaatac gttattcttt aatcctagag
      361 aaccttacct ctcctgtcac tttatggcca aatcaaaacc gggctagcac gcgtaagagc
      421 tcggtaccta tcgatagaga aatgttctgg cacctgcact tgcactgggg acagcctatt
      481 ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt actatcgatt
      541 cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg cggccgctcc
      601 aagtacctcc cgtaccttaa tattacttac ttatcatggt agcttgggct ggcgtaatag
      661 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca
      721 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
      781 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
      841 agggttgagt gttgt
//
