LOCUS       sequence-372577-        9066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human EphA7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_EphA7
FEATURES             Location/Qualifiers
     source          1..9066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1995..2014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2186..2565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2566..2769
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2771..2789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2792..2810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2820..2844
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2987..3011
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5754..5778)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5825..5848
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5885..5908
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5939..5965
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5939..5965
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5966..6313,6314..6316,6317..6925)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6969..6986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6975..7199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7332..7351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7483..7530
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7538..8560
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7538..8560
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8690..8811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8727..8746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8781..8800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8860..8876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8860..8876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8873..8895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8884..8900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8908..8914,8915..8932,8933..8938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8953..8974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     1981 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     2041 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     2101 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     2161 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     2221 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2281 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2341 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2401 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2461 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2521 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2581 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2641 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2701 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2761 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
     2821 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     2881 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
     2941 gagctcggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
     3001 aaaagcaggc tccaccatgg tttttcaaac tcggtaccct tcatggatta ttttatgcta
     3061 catctggctg ctccgctttg cacacacagg ggaggcgcag gctgcgaagg aagtactact
     3121 gctggattct aaagcacaac aaacagagtt ggagtggatt tcctctccac ccaatgggtg
     3181 ggaagaaatt agtggtttgg atgagaacta taccccgata cgaacatacc aggtgtgcca
     3241 agtcatggag cccaaccaaa acaactggct gcggactaac tggatttcca aaggcaatgc
     3301 acaaaggatt tttgtagaat tgaaattcac cctgagggat tgtaacagtc ttcctggagt
     3361 actgggaact tgcaaggaaa catttaattt gtactattat gaaacagact atgacactgg
     3421 caggaatata agagaaaacc tctatgtaaa aatagacacc attgctgcag atgaaagttt
     3481 tacccaaggt gaccttggtg aaagaaagat gaagcttaac actgaggtga gagagattgg
     3541 acctttgtcc aaaaagggat tctatcttgc ctttcaggat gtaggggctt gcatagcttt
     3601 ggtttctgtc aaagtgtact acaagaagtg ctggtccatt attgagaact tagctatctt
     3661 tccagataca gtgactggtt cagaattttc ctctttagtc gaggttcgag ggacatgtgt
     3721 cagcagtgca gaggaagaag cggaaaacgc ccccaggatg cactgcagtg cagaaggaga
     3781 atggttagtg cccattggaa aatgtatctg caaagcaggc taccagcaaa aaggagacac
     3841 ttgtgaaccc tgtggccgtg ggttctacaa gtcttcctct caagatcttc agtgctctcg
     3901 ttgtccaact cacagttttt ctgataaaga aggctcctcc agatgtgaat gtgaagatgg
     3961 gtattacagg gctccatctg acccaccata cgttgcatgc acaaggcctc catctgcacc
     4021 acagaacctc attttcaaca tcaaccaaac cacagtaagt ttggaatgga gtcctcctgc
     4081 agacaatggg ggaagaaacg atgtgaccta cagaatattg tgtaagcggt gcagttggga
     4141 gcagggcgaa tgtgttccct gtgggagtaa cattggatac atgccccagc agactggatt
     4201 agaggataac tatgtcactg tcatggacct gctagcccac gctaattata cttttgaagt
     4261 tgaagctgta aatggagttt ctgacttaag ccgatcccag aggctctttg ctgctgtcag
     4321 tatcaccact ggtcaagcag ctccctcgca agtgagcgga gtaatgaagg agagagtact
     4381 gcagcggagt gtcgagcttt cctggcagga accagagcat cccaatggag tcatcacaga
     4441 atatgaaatc aagtattacg agaaagatca aagggaacgg acctactcaa cagtaaaaac
     4501 caagtctact tcagcctcca ttaataatct gaaaccagga acagtgtatg ttttccagat
     4561 tcgggctttt actgctgctg gttatggaaa ttacagtccc agacttgatg ttgctacact
     4621 agaggaagct acaggtaaaa tgtttgaagc tacagctgtc tccagtgaac agaatcctgt
     4681 tattatcatt gctgtggttg ctgtagctgg gaccatcatt ttggtgttca tggtctttgg
     4741 cttcatcatt gggagaaggc actgtggtta tagcaaagct gaccaagaag gcgatgaaga
     4801 gctttacttt cattgcacca aaacctacat tgaccctgaa acctatgagg acccaaatag
     4861 agctgtccat caattcgcca aggagctaga tgcctcctgt attaaaattg agcgtgtgat
     4921 tggtgcagga gaattcggtg aagtctgcag tggccgtttg aaacttccag ggaaaagaga
     4981 tgttgcagta gccataaaaa ccctgaaagt tggttacaca gaaaaacaaa ggagagactt
     5041 tttgtgtgaa gcaagcatca tggggcagtt tgaccaccca aatgttgtcc atttggaagg
     5101 ggttgttaca agagggaaac cagtcatgat agtaatagag ttcatggaaa atggagccct
     5161 agatgcattt ctcaggaaac atgatgggca atttacagtc attcagttag taggaatgct
     5221 gagaggaatt gctgctggaa tgagatattt ggctgatatg ggatatgttc acagggacct
     5281 tgcagctcgc aatattcttg tcaacagcaa tctcgtttgt aaagtgtcag attttggcct
     5341 gtcccgagtt atagaggatg atccagaagc tgtctataca actactggtg gaaaaattcc
     5401 agtaaggtgg acagcacccg aagccatcca gtaccggaaa ttcacatcag ccagtgatgt
     5461 atggagctat ggaatagtca tgtgggaagt tatgtcttat ggagaaagac cttattggga
     5521 catgtcaaat caagatgtta taaaagcaat agaagaaggt tatcgtttac cagcacccat
     5581 ggactgccca gctggccttc accagctaat gttggattgt tggcaaaagg agcgtgctga
     5641 aaggccaaaa tttgaacaga tagttggaat tctagacaaa atgattcgaa acccaaatag
     5701 tctgaaaact cccctgggaa cttgtagtag gccaataagc cctcttctgg atgacccagc
     5761 tttcttgtac aaagtggttg atatcaaggc cgccgacatc accagcctgt acaagaaagt
     5821 ggggtggagc catccccagt tcgagaaggg cggaggatct ggcggcggaa gcggcggagg
     5881 aagctggtcc caccctcagt ttgagaaagg caccgagctg ggcagcacca tggccagcta
     5941 cccctacgac gtgcccgact acgccaagga caacaccgtg cccctgaagc tgatcgccct
     6001 gctggccaac ggcgagttcc actctggcga gcagctgggc gagacactgg gcatgagcag
     6061 agccgccatc aacaagcaca tccagaccct gcgggactgg ggcgtggacg tgttcacagt
     6121 gcccggcaag ggctacagcc tgcccgagcc catccagctg ctgaacgcca agcagatcct
     6181 gggccagctg gatggcggat ctgtggccgt gctgcccgtg atcgacagca ccaaccagta
     6241 tctgctcgac cggatcggcg agctgaagtc cggcgacgcc tgtatcgccg agtaccagca
     6301 ggccggcaga ggcggcaggg gcagaaagtg gttcagcccc ttcggcgcca acctgtacct
     6361 gagcatgttc tggagactgg aacagggccc agccgccgct attggcctga gcctggtcat
     6421 cggcatcgtg atggccgagg tgctgcggaa gctgggcgcc gacaaagtgc gcgtgaagtg
     6481 gcccaacgac ctgtatctgc aggaccggaa gctggccggc atcctggtgg aactgaccgg
     6541 caaaacaggc gacgccgccc agatcgtgat cggcgctggc atcaacatgg ccatgcggcg
     6601 ggtggaagag agcgtggtca accagggctg gatcaccctg caggaagccg gcatcaacct
     6661 ggaccggaac accctggccg ccatgctgat cagagagctg agagccgccc tggaactgtt
     6721 cgagcaggaa ggcctggccc cctacctgag cagatgggag aagctggaca acttcatcaa
     6781 ccggcccgtg aaactgatca tcggcgacaa agagatcttc ggcatcagcc ggggcatcga
     6841 caagcagggg gccctgctcc tggaacagga cggcatcatc aagccctgga tgggcggcga
     6901 gatcagcctg cggagcgccg agaagtgatg actcgagtct agagggcccg tttaaacccg
     6961 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7021 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7081 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7141 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     7201 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     7261 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7321 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7381 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7441 cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag
     7501 ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac
     7561 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     7621 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     7681 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     7741 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     7801 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     7861 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     7921 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     7981 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     8041 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     8101 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     8161 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     8221 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     8281 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     8341 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     8401 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     8461 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     8521 aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga
     8581 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     8641 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     8701 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8761 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8821 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8881 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8941 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9001 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9061 cggttt
//
