LOCUS       sequence-372581-        6410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent oxytocin(OT) control 
            sensor GRAB_OTmut in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Qian T, Wang H, Wang P, Geng L, Mei L, Osakada T, Wang L, Tang Y, 
            Kania A, Grinevich V, Stoop R, Lin D, Luo M, Li Y
  TITLE     A genetically encoded sensor measures temporal oxytocin release from
            different neuronal compartments.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01561-2.
  PUBMED    36593404
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01561-2."
COMMENT     Sequence Label: pAAV-hSyn-GRAB_OTmut
FEATURES             Location/Qualifiers
     source          1..6410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      581..590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     587..646
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    650..674
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1600..1621
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1727..1748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1988..2007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2537..3125
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2590..2610)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3008..3019)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3157..3633
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3356..3375)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3673..3813
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3673..3802
                     /label=AAV2 ITR
     rep_origin      3888..4343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3975..3994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4185..4206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4360..4379)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4479..4501
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4539..4557)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4625..4729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4730..4798,4799..5590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4948..4967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5761..6349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6250..6269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatgagg cggggtgggg gtgcctacct gacgaccgac
      181 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
      241 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
      301 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
      361 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
      421 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
      481 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
      541 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt gccaccatgg agacagacac
      601 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca caagtttgta
      661 caaaaaagtt ggcaccaccg gtgagggtgc gtttgcggct aactggagcg ctgaggcggt
      721 caacgggagc gcggcgccgc cgggaaccga gggcaatcgc actgccgggc cgccacagcg
      781 caacgaggcc ctggcgcggg tggaggtggc cgtgctgtgc ctcatcctgt tcctggcgct
      841 gagcggcaac gcgtgcgtgc tgctagcgct gcgcaccacg cgccacaagc actcgcgcct
      901 cttcttcttc atgaagcacc tgagcatagc cgacctggta gtggcggtgt tccaggtgct
      961 gccgcagctt ctgtgggaca tcacgttccg cttctacggg cccgacctgc tgtgccgcct
     1021 cgtcaagtac ctgcaggttg tgggcatgtt cgcgtccacc tacctgctgc tgctcatgtc
     1081 gctcgaccgc tgcctggcca tctgccagcc gctgcgctcg ctgagccgcc gcaccgaccg
     1141 cctggcggta ctcgtcacat ggctcggctg cctggtggcc agcgcgccgc aggtgcacat
     1201 cttctcgctg cgcgaggtgg ccgacggtgt cttcgactgc tgggccgttt tcattcaacc
     1261 ctgggggccc aaggcctaca tcacgtggat cacgctcgcc gtctacattg tgcccgtcat
     1321 cgtgcttgcc acctgctatg gccttatcag cttcaagatc tggcagaatt tacggctcaa
     1381 gacggcggga ggaaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa
     1441 cttccacatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca
     1501 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca
     1561 gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1621 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
     1681 catggtgaga aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1741 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccga
     1801 gggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1861 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1921 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt
     1981 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2041 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2101 caagctggag tacaacaccg gagcagcagc aaagctcatc tctaaggcca agatccgcac
     2161 ggtcaagatg accttcatcg tcgtgctggc cttcatcgtg tgctggacgc cattcttttt
     2221 cgtgcagatg tggagtgtct gggatgccga tgcgcccaag gaagcctcac ctttcatcat
     2281 cgccatgctc ctggccagcc tcaacagctg ctgcaacccc tggatctaca tgctcttcac
     2341 gggccacctc ttccaagaac ttgtgcagcg cttcctctgc tgctcattcc gccgcctgaa
     2401 aggcagccgg cctggggaga caagcgtcag caaaaagagc aactcgtcta cctttgtcct
     2461 gagccagtac agctccagcc agagaagatg ctcgcagcca tccacgctgt gagaattcga
     2521 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2581 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2641 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2701 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2761 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2821 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2881 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     2941 ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta
     3001 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3061 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3121 cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc
     3181 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3241 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3301 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3361 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3421 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     3481 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     3541 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     3601 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     3661 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3721 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     3781 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     3841 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     3901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3961 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4081 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4201 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     4261 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4321 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4381 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4441 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4501 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4681 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4741 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4801 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4861 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4921 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4981 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5041 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5101 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5161 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5221 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5281 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5341 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5401 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5461 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5521 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5581 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5641 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5701 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
