LOCUS       sequence-37261-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV
  TITLE     A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.
  JOURNAL   Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.
  PUBMED    21804536
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-31- 31"; volume: "8"; 
            issue: "9"; pages: "771-7"
COMMENT     Sequence Label: pBAD-PSmOrange
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..722)
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(290..309)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(732..749)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcttcgaatt cttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
       61 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
      121 ccgacgatgt aggcgccggg cagctgcacg gacttcttgg ccttgtaggt ggtcttgacc
      181 tcggaggtgt agtggccgcc gtccttcagc ttcagcctca tcctgatctc gcccttcagg
      241 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
      301 attacggggc cgtcggaggg gaagttggtg ccgcgcatct tcaccttgta gatgaactcg
      361 ccgtcctgca gtgaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gtagttcatc
      421 acgcgctccc acttgaagcc ctcggggaag gacagcttga agtagtcggg gatgtcggcg
      481 gggtgcttca cgtaggcctt ggagccgtag gtgaagagag gggacaggat gtcccaggcg
      541 aagggcaggg ggccaccctt ggtcaccttc agcttagcgg tctgaaagcc ctcgtagggg
      601 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggtgcc ctccatgcgc
      661 accttgaagc gcatgaactc cttgatgatg gccatattat tctcctcgcc cttgctcacc
      721 atagatctgc catggtgatg gtgatggtgg ctccccatgg ttaattcctc ctgttagccc
      781 aaaaaacggg tatggagaaa cagtagagag ttgcgataaa aagcgtcagg taggatccgc
      841 taatcttatg gataaaaatg ctatg
//
