LOCUS       sequence-37262-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV
  TITLE     A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.
  JOURNAL   Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.
  PUBMED    21804536
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-31- 31"; volume: "8"; 
            issue: "9"; pages: "771-7"
COMMENT     Sequence Label: pBAD-PSmOrange
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..93
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             103..813
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     516..535
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
       61 ttaaccatgg ggagccacca tcaccatcac catggcagat ctatggtgag caagggcgag
      121 gagaataata tggccatcat caaggagttc atgcgcttca aggtgcgcat ggagggcacc
      181 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcttt
      241 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      301 tcccctctct tcacctacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      361 tacttcaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa ctacgaggac
      421 ggcggcgtgg tgaccgtgac ccaggactcc tcactgcagg acggcgagtt catctacaag
      481 gtgaagatgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      541 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
      601 aggatgaggc tgaagctgaa ggacggcggc cactacacct ccgaggtcaa gaccacctac
      661 aaggccaaga agtccgtgca gctgcccggc gcctacatcg tcggcatcaa gttggacatc
      721 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
      781 tccaccggcg gcatggacga gctgtacaag taagaattcg aagcttggct gttttgg
//
