LOCUS       sequence-372628-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human IGF1R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_IGF1R
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(155..174)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    306..353
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             361..1383
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             361..1383
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1513..1634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1550..1569)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1604..1623
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1683..1699)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1683..1699)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1696..1718)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1707..1723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1731..1737,1738..1755,1756..1761))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1776..1797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1914..1931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2085..2673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2165..2184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2844..3635,3636..3704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3467..3486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3705..3809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3884..3903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4075..4454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4455..4658
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4609..4629
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4660..4678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4681..4699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4709..4733
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4876..4900
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9008..9032)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9079..9102
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9139..9162
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9193..9219
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             9193..9219
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(9220..9567,9568..9570,9571..10179)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(10223..10240)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(10229..10431,1..22)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
       61 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      121 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      181 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      241 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      301 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc
      361 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
      421 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
      481 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
      541 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
      601 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
      661 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
      721 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
      781 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
      841 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
      901 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
      961 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     1021 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     1081 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     1141 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     1201 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     1261 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     1321 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     1381 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     1441 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     1501 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1561 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1621 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     1681 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1741 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1801 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1861 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1921 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1981 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2041 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2101 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2161 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2221 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2281 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2341 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2401 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2461 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2521 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2581 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2641 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2701 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2761 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2821 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2881 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2941 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3001 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3061 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3121 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3181 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3241 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3301 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3361 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3421 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3481 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3541 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3601 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3661 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3721 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3781 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3841 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     3901 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     3961 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     4021 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     4081 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4141 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     4201 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4261 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     4321 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4381 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4441 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4501 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4561 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4621 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
     4681 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
     4741 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
     4801 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     4861 attctgcaga tatcaacaag tttgtacaaa aaagcaggct ccaccatgaa gtctggctcc
     4921 ggaggagggt ccccgacctc gctgtggggg ctcctgtttc tctccgccgc gctctcgctc
     4981 tggccgacga gtggagaaat ctgcgggcca ggcatcgaca tccgcaacga ctatcagcag
     5041 ctgaagcgcc tggagaactg cacggtgatc gagggctacc tccacatcct gctcatctcc
     5101 aaggccgagg actaccgcag ctaccgcttc cccaagctca cggtcattac cgagtacttg
     5161 ctgctgttcc gagtggctgg cctcgagagc ctcggagacc tcttccccaa cctcacggtc
     5221 atccgcggct ggaaactctt ctacaactac gccctggtca tcttcgagat gaccaatctc
     5281 aaggatattg ggctttacaa cctgaggaac attactcggg gggccatcag gattgagaaa
     5341 aatgctgacc tctgttacct ctccactgtg gactggtccc tgatcctgga tgcggtgtcc
     5401 aataactaca ttgtggggaa taagccccca aaggaatgtg gggacctgtg tccagggacc
     5461 atggaggaga agccgatgtg tgagaagacc accatcaaca atgagtacaa ctaccgctgc
     5521 tggaccacaa accgctgcca gaaaatgtgc ccaagcacgt gtgggaagcg ggcgtgcacc
     5581 gagaacaatg agtgctgcca ccccgagtgc ctgggcagct gcagcgcgcc tgacaacgac
     5641 acggcctgtg tagcttgccg ccactactac tatgccggtg tctgtgtgcc tgcctgcccg
     5701 cccaacacct acaggtttga gggctggcgc tgtgtggacc gtgacttctg cgccaacatc
     5761 ctcagcgccg agagcagcga ctccgagggg tttgtgatcc acgacggcga gtgcatgcag
     5821 gagtgcccct cgggcttcat ccgcaacggc agccagagca tgtactgcat cccttgtgaa
     5881 ggtccttgcc cgaaggtctg tgaggaagaa aagaaaacaa agaccattga ttctgttact
     5941 tctgctcaga tgctccaagg atgcaccatc ttcaagggca atttgctcat taacatccga
     6001 cgggggaata acattgcttc agagctggag aacttcatgg ggctcatcga ggtggtgacg
     6061 ggctacgtga agatccgcca ttctcatgcc ttggtctcct tgtccttcct aaaaaacctt
     6121 cgcctcatcc taggagagga gcagctagaa gggaattact ccttctacgt cctcgacaac
     6181 cagaacttgc agcaactgtg ggactgggac caccgcaacc tgaccatcaa agcagggaaa
     6241 atgtactttg ctttcaatcc caaattatgt gtttccgaaa tttaccgcat ggaggaagtg
     6301 acggggacta aagggcgcca aagcaaaggg gacataaaca ccaggaacaa cggggagaga
     6361 gcctcctgtg aaagtgacgt cctgcatttc acctccacca ccacgtcgaa gaatcgcatc
     6421 atcataacct ggcaccggta ccggccccct gactacaggg atctcatcag cttcaccgtt
     6481 tactacaagg aagcaccctt taagaatgtc acagagtatg atgggcagga tgcctgcggc
     6541 tccaacagct ggaacatggt ggacgtggac ctcccgccca acaaggacgt ggagcccggc
     6601 atcttactac atgggctgaa gccctggact cagtacgccg tttacgtcaa ggctgtgacc
     6661 ctcaccatgg tggagaacga ccatatccgt ggggccaaga gtgagatctt gtacattcgc
     6721 accaatgctt cagttccttc cattcccttg gacgttcttt cagcatcgaa ctcctcttct
     6781 cagttaatcg tgaagtggaa ccctccctct ctgcccaacg gcaacctgag ttactacatt
     6841 gtgcgctggc agcggcagcc tcaggacggc tacctttacc ggcacaatta ctgctccaaa
     6901 gacaaaatcc ccatcaggaa gtatgccgac ggcaccatcg acattgagga ggtcacagag
     6961 aaccccaaga ctgaggtgtg tggtggggag aaagggcctt gctgcgcctg ccccaaaact
     7021 gaagccgaga agcaggccga gaaggaggag gctgaatacc gcaaagtctt tgagaatttc
     7081 ctgcacaact ccatcttcgt gcccagacct gaaaggaagc ggagagatgt catgcaagtg
     7141 gccaacacca ccatgtccag ccgaagcagg aacaccacgg ccgcagacac ctacaacatc
     7201 actgacccgg aagagctgga gacagagtac cctttctttg agagcagagt ggataacaag
     7261 gagagaactg tcatttctaa ccttcggcct ttcacattgt accgcatcga tatccacagc
     7321 tgcaaccacg aggctgagaa gctgggctgc agcgcctcca acttcgtctt tgcaaggact
     7381 atgcccgcag aaggagcaga tgacattcct gggccagtga cctgggagcc aaggcctgaa
     7441 aactccatct ttttaaagtg gccggaacct gagaatccca atggattgat tctaatgtat
     7501 gaaataaaat acggatcaca agttgaggat cagcgagaat gtgtgtccag acaggaatac
     7561 aggaagtatg gaggggccaa gctaaaccgg ctaaacccgg ggaactacac agcccggatt
     7621 caggccacat ctctctctgg gaatgggtcg tggacagatc ctgtgttctt ctatgtccag
     7681 gccaaaacag gatatgaaaa cttcatccat ctgatcatcg ctctgcccgt cgctgtcctg
     7741 ttgatcgtgg gagggttggt gattatgctg tacgtcttcc atagaaagag aaataacagc
     7801 aggctgggga atggagtgct gtatgcctct gtgaacccgg agtacttcag cgctgctgat
     7861 gtgtacgttc ctgatgagtg ggaggtggct cgggagaaga tcaccatgag ccgggaactt
     7921 gggcaggggt cgtttgggat ggtctatgaa ggagttgcca agggtgtggt gaaagatgaa
     7981 cctgaaacca gagtggccat taaaacagtg aacgaggccg caagcatgcg tgaaaggatt
     8041 gagtttctca acgaagcttc tgtgatgaag gagttcaatt gtcaccatgt ggtgcgattg
     8101 ctgggtgtgg tgtcccaagg ccagccaaca ctggtcatca tggaactgat gacacggggc
     8161 gatctcaaaa gttatctccg gtctctgagg ccagaaatgg agaataatcc agtcctagca
     8221 cctccaagcc tgagcaagat gattcagatg gccggagaga ttgcagacgg catggcatac
     8281 ctcaacgcca ataagttcgt ccacagagac cttgctgccc ggaattgcat ggtagccgaa
     8341 gatttcacag tcaaaatcgg agattttggt atgacgcgag atatctatga gacagactat
     8401 taccggaaag gagggaaagg gctgctgccc gtgcgctgga tgtctcctga gtccctcaag
     8461 gatggagtct tcaccactta ctcggacgtc tggtccttcg gggtcgtcct ctgggagatc
     8521 gccacactgg ccgagcagcc ctaccagggc ttgtccaacg agcaagtcct tcgcttcgtc
     8581 atggagggcg gccttctgga caagccagac aactgtcctg acatgctgtt tgaactgatg
     8641 cgcatgtgct ggcagtataa ccccaagatg aggccttcct tcctggagat catcagcagc
     8701 atcaaagagg agatggagcc tggcttccgg gaggtctcct tctactacag cgaggagaac
     8761 aagctgcccg agccggagga gctggacctg gagccagaga acatggagag cgtccccctg
     8821 gacccctcgg cctcctcgtc ctccctgcca ctgcccgaca gacactcagg acacaaggcc
     8881 gagaacggcc ccggccctgg ggtgctggtc ctccgcgcca gcttcgacga gagacagcct
     8941 tacgcccaca tgaacggggg ccgcaagaac gagcgggcct tgccgctgcc ccagtcttcg
     9001 acctgcgacc cagctttctt gtacaaagtg gttgatatca aggccgccga catcaccagc
     9061 ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg atctggcggc
     9121 ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga gctgggcagc
     9181 accatggcca gctaccccta cgacgtgccc gactacgcca aggacaacac cgtgcccctg
     9241 aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct gggcgagaca
     9301 ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga ctggggcgtg
     9361 gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca gctgctgaac
     9421 gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc cgtgatcgac
     9481 agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga cgcctgtatc
     9541 gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag ccccttcggc
     9601 gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc cgctattggc
     9661 ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg cgccgacaaa
     9721 gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc cggcatcctg
     9781 gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc tggcatcaac
     9841 atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac cctgcaggaa
     9901 gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc
     9961 gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg ggagaagctg
    10021 gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat cttcggcatc
    10081 agccggggca tcgacaagca gggggccctg ctcctggaac aggacggcat catcaagccc
    10141 tggatgggcg gcgagatcag cctgcggagc gccgagaagt gatgactcga gtctagaggg
    10201 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    10261 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    10321 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    10381 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc t
//
