LOCUS       sequence-372637-        3804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DUX4 with KBM (p. E414-L424 deletion) entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei 
            GH, Varjosalo M
  TITLE     Human transcription factor protein interaction networks.
  JOURNAL   Nat Commun. 2022 Feb 9;13(1):766. doi: 10.1038/s41467-022-28341-5.
  PUBMED    35140242
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-02-9"; volume: "13(1):766. doi"; pages: " 
            10.1038/s41467-022-28341-5"
COMMENT     Sequence Label: DUX4_KBM pDonor221
FEATURES             Location/Qualifiers
     source          1..3804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(57..84)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(176..262)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     311..333
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     325..342
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     326..342
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1718..1817)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1834..1853)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1835..1853)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1858..1874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1858..1874)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1976..1995)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1987..2796
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2054..2073)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2943..3531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3432..3451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3685..3702
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
       61 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
      121 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
      181 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
      241 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
      301 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatggccct cccgacaccc
      481 tcggacagca ccctccccgc ggaagcccgg ggacgaggac ggcgacggag actcgtttgg
      541 accccgagcc aaagcgaggc cctgcgagcc tgctttgagc ggaacccgta cccgggcatc
      601 gccaccagag aacgactggc ccaggccatc ggcattccgg agcccagggt ccagatttgg
      661 tttcagaatg agaggtcacg ccagctgagg cagcaccggc gggaatctcg gccctggccc
      721 gggagacgcg gcccgccaga aggccggcga aagcggaccg ccgtcaccgg atcccagacc
      781 gccctgctcc tccgagcctt tgagaaggat cgctttccag gcatcgccgc ccgggaggag
      841 ctggccagag agacgggcct cccggagtcc aggattcaga tctggtttca gaatcgaagg
      901 gccaggcacc cgggacaggg tggcagggcg cccgcgcagg caggcggcct gtgcagcgcg
      961 gcccccggcg ggggtcaccc tgctccctcg tgggtcgcct tcgcccacac cggcgcgtgg
     1021 ggaacggggc ttcccgcacc ccacgtgccc tgcgcgcctg gggctctccc acagggtgct
     1081 ttcgtgagcc aggcagcgag ggccgccccc gcgctgcagc ccagccaggc cgcgccggca
     1141 gaggggatct cccaacctgc cccggcgcgc ggggatttcg cctacgccgc cccggctcct
     1201 ccggacgggg cgctctccca ccctcaggct cctcgctggc ctccgcaccc gggcaaaagc
     1261 cgggaggacc gggacccgca gcgcgacggc ctgccgggcc cctgcgcggt ggcacagcct
     1321 gggcccgctc aagcggggcc gcagggccaa ggggtgcttg cgccacccac gtcccagggg
     1381 agtccgtggt ggggctgggg ccggggtccc caggtcgccg gggcggcgtg ggaaccccaa
     1441 gccggggcag ctccacctcc ccagcccgcg cccccggacg cctccgcctc cgcgcggcag
     1501 gggcagatgc aaggcatccc ggcgccctcc caggcgctcc aggagccggc gccctggtct
     1561 gcactcccct gcggcctgct gctggatgag ctcctggcga gcccggagtt tctgcagcag
     1621 gcgcaacctc tcctagaaac ggaggccccg ggggagctgg aggcctcgga agaggccgcc
     1681 tcgctggaag cacccctcag cgaggaagag gagcttgacc cagctttctt gtacaaagtt
     1741 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1801 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1861 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1921 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1981 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2041 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2101 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2161 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2221 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2281 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2341 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2401 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2461 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2521 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2581 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2641 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2701 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2761 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2821 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2881 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2941 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3001 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3061 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3781 tttcccgact ggaaagcggg cagt
//
