LOCUS       sequence-372638-        3822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DUX4 entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei 
            GH, Varjosalo M
  TITLE     Human transcription factor protein interaction networks.
  JOURNAL   Nat Commun. 2022 Feb 9;13(1):766. doi: 10.1038/s41467-022-28341-5.
  PUBMED    35140242
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-02-9"; volume: "13(1):766. doi"; pages: " 
            10.1038/s41467-022-28341-5"
COMMENT     Sequence Label: DUX4 pDonor221
FEATURES             Location/Qualifiers
     source          1..3822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1037..1136)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1153..1172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1154..1172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1177..1193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1177..1193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1295..1314)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1306..2115
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1373..1392)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2262..2850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2751..2770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3004..3021
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3180..3207)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3299..3385)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3434..3456
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3448..3465
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3449..3465
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3481..3580
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cgggaatctc ggccctggcc cgggagacgc ggcccgccag aaggccggcg aaagcggacc
       61 gccgtcaccg gatcccagac cgccctgctc ctccgagcct ttgagaagga tcgctttcca
      121 ggcatcgccg cccgggagga gctggccaga gagacgggcc tcccggagtc caggattcag
      181 atctggtttc agaatcgaag ggccaggcac ccgggacagg gtggcagggc gcccgcgcag
      241 gcaggcggcc tgtgcagcgc ggcccccggc gggggtcacc ctgctccctc gtgggtcgcc
      301 ttcgcccaca ccggcgcgtg gggaacgggg cttcccgcac cccacgtgcc ctgcgcgcct
      361 ggggctctcc cacagggggc tttcgtgagc caggcagcga gggccgcccc cgcgctgcag
      421 cccagccagg ccgcgccggc agaggggatc tcccaacctg ccccggcgcg cggggatttc
      481 gcctacgccg ccccggctcc tccggacggg gcgctctccc accctcaggc tcctcgctgg
      541 cctccgcacc cgggcaaaag ccgggaggac cgggacccgc agcgcgacgg cctgccgggc
      601 ccctgcgcgg tggcacagcc tgggcccgct caagcggggc cgcagggcca aggggtgctt
      661 gcgccaccca cgtcccaggg gagtccgtgg tggggctggg gccggggtcc ccaggtcgcc
      721 ggggcggcgt gggaacccca agccggggca gctccacctc cccagcccgc gcccccggac
      781 gcctccgcct ccgcgcggca ggggcagatg caaggcatcc cggcgccctc ccaggcgctc
      841 caggagccgg cgccctggtc tgcactcccc tgcggcctgc tgctggatga gctcctggcg
      901 agcccggagt ttctgcagca ggcgcaacct ctcctagaaa cggaggcccc gggggagctg
      961 gaggcctcgg aagaggccgc ctcgctggaa gcacccctca gcgaggaaga ataccgggct
     1021 ctgctggagg agcttgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1081 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1141 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1201 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1261 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1321 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1381 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1441 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1501 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1561 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1621 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1681 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1741 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1801 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1861 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1921 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1981 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2041 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2101 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2161 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3121 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3181 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3241 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3301 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3361 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     3421 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     3481 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     3541 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cctcccgaca
     3601 ccctcggaca gcaccctccc cgcggaagcc cggggacgag gacggcgacg gagactcgtt
     3661 tggaccccga gccaaagcga ggccctgcga gcctgctttg agcggaaccc gtacccgggc
     3721 atcgccacca gagaacggct ggcccaggcc atcggcattc cggagcccag ggtccagatt
     3781 tggtttcaga atgagaggtc acgccagctg aggcagcacc gg
//
