LOCUS       sequence-372639-        9288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human EPHB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_EPHB2
FEATURES             Location/Qualifiers
     source          1..9288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2005..2024)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2196..2575
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2576..2779
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2730..2750
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2781..2799
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2802..2820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2830..2854
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2997..3021
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5986..6010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6057..6080
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6117..6140
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6171..6197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6171..6197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6198..6545,6546..6548,6549..7157)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7201..7218)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7207..7431
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7564..7583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7715..7762
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7770..8792
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7770..8792
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8922..9043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8959..8978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9013..9032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9092..9108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9092..9108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9105..9127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9116..9132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9140..9146,9147..9164,9165..9170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9185..9206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     1981 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     2041 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     2101 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     2161 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     2221 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     2281 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     2341 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     2401 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     2461 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2521 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     2581 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     2641 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     2701 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     2761 ggtctatata agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga
     2821 tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     2881 tgacctccat agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa
     2941 gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa
     3001 gtttgtacaa aaaagcaggc tccaccatgg ctctgcggag gctgggggcc gcgctgctgc
     3061 tgctgccgct gctcgccgcc gtggaagaaa cgctaatgga ctccactaca gcgactgctg
     3121 agctgggctg gatggtgcat cctccatcag ggtgggaaga ggtgagtggc tacgatgaga
     3181 acatgaacac gatccgcacg taccaggtgt gcaacgtgtt tgagtcaagc cagaacaact
     3241 ggctacggac caagtttatc cggcgccgtg gcgcccaccg catccacgtg gagatgaagt
     3301 tttcggtgcg tgactgcagc agcatcccca gcgtgcctgg ctcctgcaag gagaccttca
     3361 acctctatta ctatgaggct gactttgact cggccaccaa gaccttcccc aactggatgg
     3421 agaatccatg ggtgaaggtg gataccattg cagccgacga gagcttctcc caggtggacc
     3481 tgggtggccg cgtcatgaaa atcaacaccg aggtgcggag cttcggacct gtgtcccgca
     3541 gcggcttcta cctggccttc caggactatg gcggctgcat gtccctcatc gccgtgcgtg
     3601 tcttctaccg caagtgcccc cgcatcatcc agaatggcgc catcttccag gaaaccctgt
     3661 cgggggctga gagcacatcg ctggtggctg cccggggcag ctgcatcgcc aatgcggaag
     3721 aggtggatgt acccatcaag ctctactgta acggggacgg cgagtggctg gtgcccatcg
     3781 ggcgctgcat gtgcaaagca ggcttcgagg ccgttgagaa tggcaccgtc tgccgaggtt
     3841 gtccatctgg gactttcaag gccaaccaag gggatgaggc ctgtacccac tgtcccatca
     3901 acagccggac cacttctgaa ggggccacca actgtgtctg ccgcaatggc tactacagag
     3961 cagacctgga ccccctggac atgccctgca caaccatccc ctccgcgccc caggctgtga
     4021 tttccagtgt caatgagacc tccctcatgc tggagtggac ccctccccgc gactccggag
     4081 gccgagagga cctcgtctac aacatcatct gcaagagctg tggctcgggc cggggtgcct
     4141 gcacccgctg cggggacaat gtacagtacg caccacgcca gctaggcctg accgagccac
     4201 gcatttacat cagtgacctg ctggcccaca cccagtacac cttcgagatc caggctgtga
     4261 acggcgttac tgaccagagc cccttctcgc ctcagttcgc ctctgtgaac atcaccacca
     4321 accaggcagc tccatcggca gtgtccatca tgcatcaggt gagccgcacc gtggacagca
     4381 ttaccctgtc gtggtcccag ccggaccagc ccaatggcgt gatcctggac tatgagctgc
     4441 agtactatga gaaggagctc agtgagtaca acgccacagc cataaaaagc cccaccaaca
     4501 cggtcaccgt gcagggcctc aaagccggcg ccatctatgt cttccaggtg cgggcacgca
     4561 ccgtggcagg ctacgggcgc tacagcggca agatgtactt ccagaccatg acagaagccg
     4621 agtaccagac aagcatccag gagaagttgc cactcatcat cggctcctcg gccgctggcc
     4681 tggtcttcct cattgctgtg gttgtcatcg ccatcgtgtg taacagacgg gggtttgagc
     4741 gtgctgactc ggagtacacg gacaagctgc aacactacac cagtggccac atgaccccag
     4801 gcatgaagat ctacatcgat cctttcacct acgaggaccc caacgaggca gtgcgggagt
     4861 ttgccaagga aattgacatc tcctgtgtca aaattgagca ggtgatcgga gcaggggagt
     4921 ttggcgaggt ctgcagtggc cacctgaagc tgccaggcaa gagagagatc tttgtggcca
     4981 tcaagacgct caagtcgggc tacacggaga agcagcgccg ggacttcctg agcgaagcct
     5041 ccatcatggg ccagttcgac catcccaacg tcatccacct ggagggtgtc gtgaccaaga
     5101 gcacacctgt gatgatcatc accgagttca tggagaatgg ctccctggac tcctttctcc
     5161 ggcaaaacga tgggcagttc acagtcatcc agctggtggg catgcttcgg ggcatcgcag
     5221 ctggcatgaa gtacctggca gacatgaact atgttcaccg tgacctggct gcccgcaaca
     5281 tcctcgtcaa cagcaacctg gtctgcaagg tgtcggactt tgggctctca cgctttctag
     5341 aggacgatac ctcagacccc acctacacca gtgccctggg cggaaagatc cccatccgct
     5401 ggacagcccc ggaagccatc cagtaccgga agttcacctc ggccagtgat gtgtggagct
     5461 acggcattgt catgtgggag gtgatgtcct atggggagcg gccctactgg gacatgacca
     5521 accaggatgt aatcaatgcc attgagcagg actatcggct gccaccgccc atggactgcc
     5581 cgagcgccct gcaccaactc atgctggact gttggcagaa ggaccgcaac caccggccca
     5641 agttcggcca aattgtcaac acgctagaca agatgatccg caatcccaac agcctcaaag
     5701 ccatggcgcc cctctcctct ggcatcaacc tgccgctgct ggaccgcacg atccccgact
     5761 acaccagctt taacacggtg gacgagtggc tggaggccat caagatgggg cagtacaagg
     5821 agagcttcgc caatgccggc ttcacctcct ttgacgtcgt gtctcagatg atgatggagg
     5881 acattctccg ggttggggtc actttggctg gccaccagaa aaaaatcctg aacagtatcc
     5941 aggtgatgcg ggcgcagatg aaccagattc agtctgtgga ggttgaccca gctttcttgt
     6001 acaaagtggt tgatatcaag gccgccgaca tcaccagcct gtacaagaaa gtggggtgga
     6061 gccatcccca gttcgagaag ggcggaggat ctggcggcgg aagcggcgga ggaagctggt
     6121 cccaccctca gtttgagaaa ggcaccgagc tgggcagcac catggccagc tacccctacg
     6181 acgtgcccga ctacgccaag gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca
     6241 acggcgagtt ccactctggc gagcagctgg gcgagacact gggcatgagc agagccgcca
     6301 tcaacaagca catccagacc ctgcgggact ggggcgtgga cgtgttcaca gtgcccggca
     6361 agggctacag cctgcccgag cccatccagc tgctgaacgc caagcagatc ctgggccagc
     6421 tggatggcgg atctgtggcc gtgctgcccg tgatcgacag caccaaccag tatctgctcg
     6481 accggatcgg cgagctgaag tccggcgacg cctgtatcgc cgagtaccag caggccggca
     6541 gaggcggcag gggcagaaag tggttcagcc ccttcggcgc caacctgtac ctgagcatgt
     6601 tctggagact ggaacagggc ccagccgccg ctattggcct gagcctggtc atcggcatcg
     6661 tgatggccga ggtgctgcgg aagctgggcg ccgacaaagt gcgcgtgaag tggcccaacg
     6721 acctgtatct gcaggaccgg aagctggccg gcatcctggt ggaactgacc ggcaaaacag
     6781 gcgacgccgc ccagatcgtg atcggcgctg gcatcaacat ggccatgcgg cgggtggaag
     6841 agagcgtggt caaccagggc tggatcaccc tgcaggaagc cggcatcaac ctggaccgga
     6901 acaccctggc cgccatgctg atcagagagc tgagagccgc cctggaactg ttcgagcagg
     6961 aaggcctggc cccctacctg agcagatggg agaagctgga caacttcatc aaccggcccg
     7021 tgaaactgat catcggcgac aaagagatct tcggcatcag ccggggcatc gacaagcagg
     7081 gggccctgct cctggaacag gacggcatca tcaagccctg gatgggcggc gagatcagcc
     7141 tgcggagcgc cgagaagtga tgactcgagt ctagagggcc cgtttaaacc cgctgatcag
     7201 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     7261 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     7321 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     7381 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     7441 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     7501 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7561 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7621 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7681 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
     7741 ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg
     7801 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     7861 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     7921 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     7981 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     8041 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     8101 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     8161 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     8221 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     8281 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     8341 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     8401 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     8461 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     8521 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     8581 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     8641 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     8701 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     8761 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
     8821 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8881 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     9061 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcgggga
//
